Droplet actuator apparatus and system

• US 9,446,404 B2
• Filed: 07/25/2012
• Issued: 09/20/2016
• Est. Priority Date: 07/25/2011
• Status: Expired due to Fees

• Current Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Original Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Inventors: Bauer, William Craig, Sista, Ramakrishna, Bort, Donovan E.
• Primary Examiner(s): DIETERLE, JENNIFER M
• Application Number:
  US14/234,855
• Publication Number:
  US 20140231257A1
• Time in Patent Office: 1,518 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension
• CPC Class Codes: B23P 11/02 by first expanding and then shrinking or vice versa, e.g. by using pressure fluids; by making force fits
• CPC Class Codes: Y10T 29/49945 by driven force fit

Abstract

Droplet actuator apparatus and system are provided. An apparatus comprises: a microfluidics system having an insertion slot for insertion of a droplet actuator; a bottom plate against which the droplet actuator would slide when inserting the droplet actuator into the insertion slot; and means for forcing a substrate in the droplet actuator parallel to the bottom plate, wherein warpage in the substrate is corrected.

Claims

1. An apparatus, comprising:

a droplet actuator for insertion into an insertion slot of a microfluidics system;

a top substrate and a bottom substrate defining a gap in the droplet actuator;

droplet operations electrodes in the bottom substrate for transporting a droplet along the gap;

a ramped edge in the droplet actuator to reduce friction during the insertion into the insertion slot of the microfluidics system; and

means for seating a force applied to the top substrate that corrects warpage in the bottom substrate.

2. The apparatus according to claim 1, wherein the means for seating the force comprises at least one detent in a top surface of the top substrate.

3. The apparatus according to claim 2, further comprising a gap setting feature aligned with the at least one detent, the gap setting feature establishing a minimum height of the gap between the top substrate and the bottom substrate.

4. The apparatus according to claim 1, further comprising a gap setting feature that establishes a minimum height of the gap between the top substrate and the bottom substrate.

5. The apparatus according to claim 1, further comprising a gap setting feature protruding from the top substrate that establishes a minimum height of the gap between the top substrate and the bottom substrate.

6. An apparatus comprising:

a droplet actuator for insertion into an insertion slot of a microfluidics system;

a top substrate and a bottom substrate defining a gap in the droplet actuator;

droplet operations electrodes in the bottom substrate for transporting a droplet along the gap;

a ramp proximate a leading edge of the top substrate to reduce friction during the insertion into the insertion slot of the microfluidics system; and

means for seating a force applied to the top substrate that corrects warpage in the bottom substrate.

16. The apparatus according to claim 6, wherein the means for seating the force comprises at least one detent in a top surface of the top substrate.

17. The apparatus according to claim 16, further comprising a gap setting feature aligned with the at least one detent, the gap setting feature establishing a minimum height of the gap between the top substrate and the bottom substrate.

18. The apparatus according to claim 6, further comprising a gap setting feature that establishes a minimum height of the gap between the top substrate and the bottom substrate.

19. The apparatus according to claim 6, further comprising a gap setting feature protruding from the top substrate that establishes a minimum height of the gap between the top substrate and the bottom substrate.

7. An apparatus comprising:

a droplet actuator for insertion into an insertion slot of a microfluidics system;

a top substrate and a bottom substrate defining a gap in the droplet actuator;

droplet operations electrodes in the bottom substrate for transporting a droplet along the gap;

multiple detents in a top surface of the top substrate for seating a force applied to the top substrate that corrects warpage in the bottom substrate; and

multiple ramped surfaces in the top substrate of the droplet actuator, each ramped surface aligned to lead a corresponding one of the multiple detents, the multiple ramped surfaces reducing friction during the insertion of the droplet actuator into the insertion slot of the microfluidics system.

20. The apparatus according to claim 7, further comprising a gap setting feature aligned with the multiple detents, the gap setting feature establishing a minimum height of the gap between the top substrate and the bottom substrate.

21. The apparatus according to claim 7, further comprising a gap setting feature that establishes a minimum height of the gap between the top substrate and the bottom substrate.

22. The apparatus according to claim 7, further comprising a gap setting feature protruding from the top substrate that establishes a minimum height of the gap between the top substrate and the bottom substrate.

8. An apparatus, comprising:

a microfluidics system having a droplet actuator inserted into an insertion slot;

a bottom plate against which the droplet actuator slides as the droplet actuator inserts into the insertion slot;

a spring plunger applying a force to a substrate of the droplet actuator; and

a detent in the substrate of the droplet actuator into which a tip of the spring plunger seats,

wherein the spring plunger forces the substrate parallel to the bottom plate to correct warpage.

9. The apparatus according to claim 8, further comprising a gap setting feature establishing a minimum height of a droplet operations gap in the droplet actuator.

10. The apparatus according to claim 8, further comprising a gap setting feature protruding from the substrate that establishes a minimum height of a droplet operations gap in the droplet actuator.

11. The apparatus according to claim 8, further comprising a ramped edge along the droplet actuator to reduce friction during the insertion into the insertion slot of the microfluidics system.

12. The apparatus according to claim 8, further comprising a ramp proximate a leading edge of the substrate to reduce friction during the insertion into the insertion slot of the microfluidics system.

13. The apparatus according to claim 8, further comprising multiple detents in a top surface of the substrate.

14. The apparatus according to claim 8, further comprising:

multiple spring plungers applying multiple forces to the substrate of the droplet actuator; and

multiple detents in a top surface of the substrate of the droplet actuator,

wherein each tip of a corresponding one of the multiple spring plungers seats into a corresponding one of the multiple detents.

15. The apparatus according to claim 14, further comprising multiple ramped surfaces in the substrate of the droplet actuator, each one of the multiple ramped surfaces aligned to lead the corresponding one of the multiple detents, each of the multiple ramped surfaces reducing friction during the insertion of the droplet actuator into the insertion slot of the microfluidics system.