PH20 polypeptide variants, formulations and uses thereof

• US 9,447,401 B2
• Filed: 12/28/2012
• Issued: 09/20/2016
• Est. Priority Date: 12/30/2011
• Status: Active Grant

• Current Assignee: Halozyme, Inc. (Halozyme Therapeutics, Inc.)
• Original Assignee: Halozyme, Inc. (Halozyme Therapeutics, Inc.)
• Inventors: Wei, Ge, Shepard, H. Michael, Zhao, Qiping, Connor, Robert James
• Primary Examiner(s): Rao, Manjunath
• Assistant Examiner(s): LIU, SAMUEL W
• Application Number:
  US13/694,731
• Publication Number:
  US 20130302275A1
• Time in Patent Office: 1,362 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 38/28 Insulins
• CPC Class Codes: A61K 38/47 acting on glycosyl compounds (3.2), e.g. cellulases, lactases
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 47/10 Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 27/02 Ophthalmic agents
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 3/04 Anorexiants; Antiobesity agents
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
• CPC Class Codes: C12N 9/2474 Hyaluronoglucosaminidase (3.2.1.35), i.e. hyaluronidase
• CPC Class Codes: C12Q 1/34 involving hydrolase
• CPC Class Codes: C12Y 302/01035 Hyaluronoglucosaminidase (3.2.1.35), i.e. hyaluronidase
• CPC Class Codes: G01N 2333/926 acting on alpha -1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
• CPC Class Codes: G01N 2333/928 : acting on alpha -1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
• CPC Class Codes: Y02A 50/30 : Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Modified PH20 hyaluronidase polypeptides, including modified polypeptides that exhibit increased stability and/or increased activity, are provided. Also provided are compositions and formulations and uses thereof.

Claims

1. A modified PH20 polypeptide that exhibits increased stability in the presence of a phenolic preservative, comprising an amino acid replacement in an unmodified PH20 polypeptide, wherein:

the amino acid replacement confers the increased stability;

the amino acid replacement is replacement with proline at a position corresponding to position 204 in a PH20 polypeptide with reference to amino acid residue positions set forth in SEQ ID NO;

3;

corresponding amino acid positions are identified by alignment of the modified PH20 polypeptide with the polypeptide whose sequence is set forth in SEQ ID NO;

3;

the modified PH20 polypeptide has at least 85% sequence identity to the sequence of amino acids set forth in SEQ ID NO;

3 or 7; and

stability is increased compared to the unmodified polypeptide without the amino acid replacement, wherein stability is measured by resistance to denaturation in the presence of a phenolic preservative.

2. The modified PH20 polypeptide of claim 1, wherein the unmodified PH20 polypeptide is a soluble PH20 polypeptide that consists of the sequence of amino acids set forth in any of SEQ ID NOS:

3, 7 and 32-66.

3. The modified PH20 polypeptide of claim 1, wherein:

the unmodified PH20 polypeptide consists of the sequence of amino acids set forth in SEQ ID NO;

3 or 7; and

the modified PH20 polypeptide has at least 95% sequence identity to SEQ ID NO;

3.

4. The modified PH20 polypeptide of claim 1, that has only up to 10 amino acid replacements compared to the polypeptide of SEQ ID NO:

7 or 3.

5. The modified PH20 polypeptide of claim 1, wherein the modified PH20 polypeptide exhibits at least 15% of the hyaluronidase activity in the presence of the phenolic preservative(s) for at least 4 hours compared to the hyaluronidase activity in the absence of the phenolic preservative(s), wherein the activity is compared under the same conditions except for the presence of the phenolic preservative(s).

6. The modified PH20 polypeptide of claim 1, wherein the modified PH20 polypeptide is stable in the presence of an anti-microbially effective amount of one or more phenolic preservatives.

7. The modified PH20 polypeptide of claim 6, wherein the anti-microbially effective amount is a total amount of one or more phenolic preservative agents as a percentage (%) of mass concentration (w/v) that is from about 0.05% to 0.6%.

8. The modified PH20 polypeptide of claim 1, wherein the phenolic preservative is selected from among one or more of phenol, metacresol (m-cresol), benzyl alcohol and a paraben.

9. The modified PH20 polypeptide of claim 1, wherein the preservative is a phenolic preservative that is m-cresol, phenol, or a combination of m-cresol and phenol.

10. The modified PH20 polypeptide of claim 1, further comprising at least one amino acid replacement at the amino acid position selected from the group consisting of 58, 10, 12, 20, 22, 26, 34, 36, 46, 50, 52, 68, 70, 74, 82, 83, 84, 86, 97, 127, 131, 138, 142, 143, 144, 166, 169, 174, 193, 195, 196, 205, 206, 213, 219, 234, 237, 238, 240, 249, 261, 267, 277, 279, 291, 309, 310, 314, 315, 317, 318, 347, 367, 375, 376, 399, 401, 407, 416, 419, 421, 431, 433, 439, 440, 443 and 445 with reference to amino acid positions set forth in SEQ ID NO:

3, wherein amino acid position is identified by alignment of the modified PH20 polypeptide with SEQ ID NO;

3.

11. The modified PH20 polypeptide of claim 10, comprising at least one amino acid replacement selected from the group consisting of:

G at a position corresponding to position 10;

K at a position corresponding to position 12;

S at a position corresponding to position 20;

T at a position corresponding to position 22;

M at a position corresponding to position 26;

W at a position corresponding to position 34;

N at a position corresponding to position 36;

L at a position corresponding to position 46;

M at a position corresponding to position 50;

T at a position corresponding to position 52;

S at a position corresponding to position 52;

C at a position corresponding to position 58;

K at a position corresponding to position 58;

R at a position corresponding to position 58;

N at a position corresponding to position 58;

Y at a position corresponding to position 58;

P at a position corresponding to position 58;

H at a position corresponding to position 58;

P at a position corresponding to position 68;

V at a position corresponding to position 70;

E at a position corresponding to position 74;

L at a position corresponding to position 82;

N at a position corresponding to position 82;

V at a position corresponding to position 83;

Q at a position corresponding to position 83;

S at a position corresponding to position 83;

G at a position corresponding to position 83;

N at a position corresponding to position 84;

A at a position corresponding to position 86;

K at a position corresponding to position 86;

E at a position corresponding to position 97;

L at a position corresponding to position 97;

R at a position corresponding to position 127;

R at a position corresponding to position 131;

L at a position corresponding to position 138;

K at a position corresponding to position 142;

N at a position corresponding to position 142;

P at a position corresponding to position 142;

S at a position corresponding to position 142;

T at a position corresponding to position 142;

G at a position corresponding to position 143;

K at a position corresponding to position 143;

T at a position corresponding to position 144;

Q at a position corresponding to position 166;

T at a position corresponding to position 166;

L at a position corresponding to position 169;

G at a position corresponding to position 174;

N at a position corresponding to position 174;

Q at a position corresponding to position 193;

T at a position corresponding to position 195;

N at a position corresponding to position 195;

E at a position corresponding to position 196;

R at a position corresponding to position 196;

A at a position corresponding to position 205;

E at a position corresponding to position 205;

I at a position corresponding to position 206;

A at a position corresponding to position 213;

I at a position corresponding to position 219;

M at a position corresponding to position 234;

T at a position corresponding to position 237;

H at a position corresponding to position 238;

Q at a position corresponding to position 240;

V at a position corresponding to position 249;

A at a position corresponding to position 26t;

K at a position corresponding to position 261;

T at a position corresponding to position 267;

K at a position corresponding to position 277;

H at a position corresponding to position 279;

V at a position corresponding to position 279;

V at a position corresponding to position 291;

E at a position corresponding to position 309;

Q at a position corresponding to position 310;

Y at a position corresponding to position 314;

Y at a position corresponding to position 315;

N at a position corresponding to position 317;

W at a position corresponding to position 317;

D at a position corresponding to position 318;

G at a position corresponding to position 347;

A at a position corresponding to position 367;

R at a position corresponding to position 375;

R at a position corresponding to position 376;

V at a position corresponding to position 399;

E at a position corresponding to position 401;

A at a position corresponding to position 407;

L at a position corresponding to position 416;

K at a position corresponding to position 419;

H at a position corresponding to position 421;

E at a position corresponding to position 431;

T at a position corresponding to position 433;

V at a position corresponding to position 433;

C at a position corresponding to position 439;

P at a position corresponding to position 440;

G at a position corresponding to position 443; and

N at a position corresponding to position 445, with reference to amino acid sequence of SEQ ID NO;

3.

12. The modified PH20 polypeptide of claim 11, comprising at least one amino acid replacement selected from the group consisting of:

T at a position corresponding to position 52, K at a position corresponding to position 58, R at a position corresponding to position 58, P at a position corresponding to position 68, V at a position corresponding to position 83, P at a position corresponding to position 204, A at a position corresponding to position 261, T at a position corresponding to position 267, K at a position corresponding to position 277 and H at a position corresponding to position 421, with reference to amino acid residue positions set forth in SEQ ID NO;

3.

13. The modified PH20 polypeptide of claim 12, comprising replacement with R at a position corresponding to position 58 in a PH20 polypeptide with reference to amino acid positions set forth in SEQ ID NO:

3.

14. The modified PH20 polypeptide of claim 1 that consists of a sequence of amino acid residues having at least 98% sequence identity to SEQ ID NO:

49 and comprises replacement with P at a position corresponding to position 204 in a PH20 polypeptide with reference to amino acid residue positions set forth in SEQ ID NO;

49.

15. The modified PH20 polypeptide of claim 1 that is purified or isolated.

16. The modified PH20 polypeptide of claim 1 that is further modified by a modification selected from among glycosylation, sialation, albumination, farnysylation, carboxylation, hydroxylation and phosphorylation.

17. The modified PH20 polypeptide of claim 1, wherein the modified PH20 polypeptide is glycosylated, whereby the polypeptide comprises at least an N-acetylglucosamine moiety linked to each of at least three asparagine (N) residues.

18. The modified PH20 polypeptide of claim 17, wherein the three asparagine residues correspond to amino acid residues 200, 333 and 358 of SEQ ID NO:

3.

19. The modified PH20 polypeptide of claim 1 that is modified by conjugation to a polymer.

20. The modified PH20 polypeptide of claim 19, wherein the polymer is dextran or PEG.

21. The modified PH20 polypeptide of claim 1, wherein the modified PH20 polypeptide is conjugated to a moiety selected from among a multimerization domain, toxin, detectable label or drug.

22. A pharmaceutical composition, comprising the modified PH20 polypeptide of claim 1.

23. The pharmaceutical composition of claim 22, wherein the pharmaceutical composition comprises a pharmaceutically acceptable excipient.

24. The pharmaceutical composition of claim 22, comprising an anti-microbially effective amount of a preservative or mixture of preservatives.

25. The pharmaceutical composition of claim 24, comprising at least one phenolic preservative.

26. The pharmaceutical composition of claim 25, wherein the preservative(s) is(are) selected from among phenol, metacresol (m-cresol), benzyl alcohol, and a paraben.

27. The pharmaceutical composition of claim 24, wherein the anti-microbially effective amount is a total amount of one or more preservative agents as a percentage (%) of mass concentration (w/v) that is or is between 0.05% to 0.6%.

28. The pharmaceutical composition of claim 22, comprising a therapeutically active agent.

29. The pharmaceutical composition of claim 28, wherein the therapeutic agent is a polypeptide, a protein, a nucleic acid, a drug, a small molecule or an organic molecule.

30. The pharmaceutical composition of claim 28, wherein the therapeutically active agent is selected from the group consisting of a chemotherapeutic agent, an analgesic agent, an anti-inflammatory agent, an antimicrobial agent, an amoebicidal agent, a trichomonacidal agent, an anti-parkinson agent, an anti-malarial agent, an anticonvulsant agent, an anti-depressant agent, and antiarthritics agent, an anti-fungal agent, an antihypertensive agent, an antipyretic agent, an anti-parasite agent, an antihistamine agent, an alpha-adrenergic agonist agent, an alpha blocker agent, an anesthetic agent, a bronchial dilator agent, a biocide agent, a bactericide agent, a bacteriostat agent, a beta adrenergic blocker agent, a calcium channel blocker agent, a cardiovascular drug agent, a contraceptive agent, a decongestant agent, a diuretic agent, a depressant agent, a diagnostic agent, a electrolyte agent, a hypnotic agent, a hormone agent, a hyperglycemic agent, a muscle relaxant agent, a muscle contractant agent, an ophthalmic agent, a parasympathomimetic agent, a psychic energizer agent, a sedative agent, a sympathomimetic agent, a tranquilizer agent, an urinary agent, a vaginal agent, a viricide agent, a vitamin agent, a non-steroidal anti-inflammatory agent, an angiotensin converting enzyme inhibitor agent, and a sleep inducer.

31. The pharmaceutical composition of claim 28, wherein the therapeutic agent is selected from the group consisting of Adalimumabs, Agalsidase Betas, Alefacepts, Ampicillins, Anakinras, Antipoliomyelitic Vaccines, Anti-Thymocytes, Azithromycins, Becaplermins, Caspofungins, Cefazolins, Cefepimes, Cefotetans, Ceftazidimes, Ceftriaxones, Cetuximabs, Cilastatins, Clavulanic Acids, Clindamycins, Darbepoetin Alfas, Daclizumabs, Diphtheria, Diphtheria antitoxins, Diphtheria Toxoids, Efalizumabs, Epinephrines, Erythropoietin Alphas, Etanercepts, Filgrastims, Fluconazoles, Follicle-Stimulating Hormones, Follitropin Alphas, Follitropin Betas, Fosphenytoins, Gadodiamides, Gadopentetates, Gatifloxacins, Glatiramers, GM-CSFs, Goserelin acetates, Granisetrons, Haemophilus Influenza B'"'"'s, Haloperidols, Hepatitis vaccines, Hepatitis A Vaccines, Hepatiti B Vaccines, Ibritumomab Tiuxetans, Ibritumomabs, Tiuxetans, Immunoglobulins, Hemophilus influenza vaccines, Influenza Virus Vaccines, Infliximabs, Insulin lispro, 75% neutral protamine lispro (NPL)/25% insulin lispro, 50% neutral protamine Hagedorn (NPH)/50% regular insulin, 70% NPH/30% regular insulin;

Regular insulin, NPH insulin, Ultra insulin, Ultralente insulin, and Insulin Glargines, Interferons, Interferon alpha, Interferon Betas, Interferon Gammas, Interferon alpha-2a, Interferon alpha 2-b, Interferon Alphacon, Interferon alpha-n, Interferon Betas, Interferon Beta-1 a'"'"'s, Interferon Gammas, Interferon alpha-con, Iodixanols, Iohexols, Iopamidols, Ioversols, Ketorolacs, Laronidases, Levofloxacins, Lidocaines, Linezolids, Lorazepams, Measles Vaccines, Measles virus, Mumps viruses, Measles-Mumps-Rubella Virus Vaccines, Rubella vaccines, Medroxyprogesterones, Meropenems, Methylprednisolones, Midazolams, Morphines, Octreotides, Omalizumabs, Ondansetrons, Palivizumabs, Pantoprazoles, Pegaspargases, Pegfilgrastims, Peg-Interferon Alpha-2a'"'"'s, Peg-Interferon Alpha-2b'"'"'s, Pegvisomants, Pertussis vaccines, Piperacillins, Pneumococcal Vaccines and Pneumococcal Conjugate Vaccines, Promethazines, Reteplases, Somatropins, Sulbactams, Sumatriptans, Tazobactams, Tenecteplases, Tetanus Purified Toxoids, Ticarcillins, Tositumomabs, Triamcinolones, Triamcinolone Acetonides, Triamcinolone hexacetonides, Vancomycins, Varicella Zoster immunoglobulins, Varicella vaccines, other vaccines, Alemtuzumabs, Alitretinoins, Allopurinols, Altretamines, Amifostines, Anastrozoles, Arsenics, Arsenic Trioxides, Asparaginases, Bacillus Calmette-Guerin (BCG) vaccines, BCG Live, Bexarotenes, Bleomycins, Busulfans, Busulfan intravenous, Busulfan orals, Calusterones, Capecitabines, Carboplatins, Carmustines, Carmustines with Polifeprosans, Celecoxibs, Chlorambucils, Cisplatins, Cladribines, Cyclophosphamides, Cytarabines, Cytarabine liposomals, Dacarbazines, Dactinomycins, Daunorubicin liposomals, Daunorubicins, Denileukin Diftitoxes, Dexrazoxanes, Docetaxels, Doxorubicins, Doxorubicin liposomals, Dromostanolone propionates, Elliotts B Solutions, Epirubicins, Epoetin alfas, Estramustines, Etoposide phosphates, Exemestanes, Floxuridines, Fludarabines, Fluorouracils, Fulvestrants, Gemcitabines, Gemtuzumabs, Ozogamicins, Gemtuzumab ozogamicins, Hydroxyureas, Idarubicins, Ifosfamides, Imatinib mesylates, Irinotecans, Letrozoles, Leucovorins, Levamisoles, Lomustines, Mechlorethamines, Nitrogen mustards, Megestrols, Megestrol acetates, Melphalans, Mercaptopurines, Mesnas, Methotrexates, Methoxsalens, Mitomycins, Mitomycin C'"'"'s, Mitotanes, Mitoxantrones, Nandrolones, Nandrolone Phenpropionates, Nofetumomabs, Oprelvekins, Oxaliplatins, Paclitaxels, Pamidronates, Pegademases, Pentostatins, Pipobromans, Plicamycins, Porfimer sodiums, Procarbazines, Quinacrines, Rasburicases, Rituximabs, Sargramostims, Streptozocins, Talcs, Tamoxifens, Temozolomides, Teniposides, Testolactones, Thioguanines, Triethylenethiophosphoramides (Thiotepas), Topotecans, Toremifenes, Trastuzumabs, Tretinoins, Uracil Mustards, Valrubicins, Vinblastines, Vincristines, Vinorelbines, Zoledronates, Acivicins, Aclarubicins, Acodazoles, Acronines, Adozelesins, Retinoic Acids, 9-Cis-Retinoic Acids, Alvocidibs, Ambazones, Ambomycins, Ametantrones, Aminoglutethimides, Amsacrines, Anaxirones, Ancitabines, Anthramycins, Apaziquones, Argimesnas, Asperlins, Atrimustines, Azacitidines, Azetepas, Azotomycins, Banoxantrones, Batabulins, Batimastats, Benaxibines, Bendamustines, Benzodepas, Bicalutamides, Bietaserpines, Biricodars, Bisantrenes, Bisnafide Dimesylates, Bizelesins, Bortezomibs, Brequinars, Bropirimines, Budotitanes, Cactinomycins, Canertinibs, Caracemides, Carbetimers, Carboquones, Carmofurs, Carubicins, Carzelesins, Cedefingols, Cemadotins, Cioteronels, Cirolemycins, Clanfenurs, Clofarabines, Crisnatols, Decitabines, Dexniguldipines, Dexormaplatins, Dezaguanines, Diaziquones, Dibrospidiums, Dienogests, Dinalins, Disermolides, Dofequidars, Doxifluridines, Droloxifenes, Duazomycins, Ecomustines, Edatrexates, Edotecarins, Eflomithines, Elacridars, Elinafides, Elsamitrucins, Emitefurs, Enloplatins, Enpromates, Enzastaurins, Epipropidines, Eptaloprosts, Erbulozoles, Esorubicins, Etanidazoles, Etoglucids, Etoprines, Exisulinds, Fadrozotes, Fazarabines, Fenretinides, Fluoxymesterones, Flurocitabines, Fosquidones, Fostriecins, Fotretamines, Galarubicins, Galocitabines, Geroquinols, Gimatecans, Gimeracils, Gloxazones, Glufosfamides, Ilmofosines, Ilomastats, Imexons, Improsulfans, Indisulams, Inproquones, Interleukins, Interleukin-2s, recombinant Interleukins, Intoplicines, Iobenguanes, Iproplatins, Irsogladines, Ixabepilones, Ketotrexates, L-Alanosines, Lanreotides, Lapatinibs, Ledoxantrones, Leuprolides, Lexacalcitols, Liarozoles, Lobaplatins, Lometrexols, Lonafamibs, Losoxantrones, Lurtotecans, Mafosfamides, Mannosulfans, Marimastats, Masoprocols, Maytansines, Melengestrols, Menogarils, Mepitiostanes, Metesinds, Metomidates, Metoprines, Meturedepas, Miboplatins, Miproxifenes, Misonidazoles, Mitindomides, Mitocarcins, Mitocromins, Mitoflaxones, Mitogillins, Mitoguazones, Mitomalcins, Mitonafides, Mitoquidones, Mitospers, Mitozolomides, Mivobulins, Mizoribines, Mofarotenes, Mopidamols, Mubritinibs, Mycophenolic Acids, Nedaplatins, Nelarabines, Nemorubicins, Nitracrines, Nocodazoles, Nogalamycins, Nolatrexeds, Nortopixantrones, Ormaplatins, Ortataxels, Oteracils, Oxisurans, Oxophenarsines, Patupilones, Peldesines, Peliomycins, Pelitrexols, Pemetrexeds, Pentamustines, Peptomycins, Perfosfamides, Perifosines, Picoplatins, Pinafides, Piposulfans, Pirfenidones, Piroxantrones, Pixantrones, Plevitrexeds, Plomestanes, Porfiromycins, Prednimustines, Propamidines, Prospidiums, Pumitepas, Puromycins, Pyrazofurins, Ranimustines, Riboprines, Ritrosulfans, Rogletimides, Roquinimexs, Sabarubicins, Safingols, Satraplatins, Sebriplatins, Semustines, Simtrazenes, Sizofirans, Sobuzoxanes, Sorafenibs, Sparfosates, Sparfosic Acids, Sparsomycins, Spirogermaniums, Spiromustines, Spiroplatins, Squalamines, Streptonigrins, Streptovarycins, Sufosfamides, Sulofenurs, Tacedinalines, Talisomycins, Tallimustines, Tariquidars, Tauromustines, Tecogalans, Tegafurs, Teloxantrones, Temoporfins, Teroxirones, Thiamiprines, Tiamiprines, Tiazofurins, Tilomisoles, Tilorones, Timcodars, Timonacics, Tirapazamines, Topixantrones, Trabectedins, Trestolones, Triciribines, Trilostanes, Trimetrexates, Triplatin Tetranitrates, Triptorelins, Trofosfamides, Tubulozotes, Ubenimexs, Uredepas, Valspodars, Vapreotides, Verteporfins, Vindesines, Vinepidines, Vinflunines, Vinformides, Vinglycinates, Vinleucinols, Vinleurosines, Vinrosidines, Vintriptols, Vinzolidines, Vorozoles, Xanthomycin A'"'"'s, Guamecyclines, Zeniplatins, Zilascorbs [2-H], Zinostatins, Zorubicins, Zosuquidars, Acetazolamides, Acyclovirs, Adipiodones, Alatrofloxacins, Alfentanils, Allergenic extracts, Alpha 1-proteinase inhibitors, Alprostadils, Amikacins, Amino acids, Aminocaproic acids, Aminophyllines, Amitriptylines, Amobarbitals, Amrinones, Analgesics, Anti-poliomyelitic vaccines, Anti-rabic serums, Anti-tetanus immunoglobulins, tetanus vaccines, Antithrombin Ills, Antivenom serums, Argatrobans, Arginines, Ascorbic acids, Atenolols, Atracuriums, Atropines, Aurothioglucoses, Azathioprines, Aztreonams, Bacitracins, Baclofens, Basiliximabs, Benzoic acids, Benztropines, Betamethasones, Biotins, Bivalirudins, Botulism antitoxins, Bretyliums, Bumetanides, Bupivacaines, Buprenorphines, Butorphanols, Calcitonins, Calcitriols, Calciums, Capreomycins, Carboprosts, Carnitines, Cefamandoles, Cefoperazones, Cefotaximes, Cefoxitins, Ceftizoximes, Cefuroximes, Chloramphenicols, Chloroprocaines, Chloroquines, Chlorothiazides, Chlorpromazines, Chondroitinsulfuric acids, Choriogonadotropin alfas, Chromiums, Cidofovirs, Cimetidines, Ciprofloxacins, Cisatracuriums, Clonidines, Codeines, Colchicines, Colistins, Collagens, Corticorelin ovine triflutates, Corticotrophins, Cosyntropins, Cyanocobalamins, Cyclosporines, Cysteines, Dacliximabs, Dalfopristins, Dalteparins, Danaparoids, Dantrolenes, Deferoxamines, Desmopressins, Dexamethasones, Dexmedetomidines, Dexpanthenols, Dextrans, Iron dextrans, Diatrizoic acids, Diazepams, Diazoxides, Dicyclomines, Digibinds, Digoxins, Dihydroergotamines, Diltiazems, Diphenhydramines, Dipyridamoles, Dobutamines, Dopamines, Doxacuriums, Doxaprams, Doxercalciferols, Doxycyclines, Droperidols, Dyphyllines, Edetic acids, Edrophoniums, Enalaprilats, Ephedrines, Epoprostenols, Ergocalciferols, Ergonovines, Ertapenems, Ery˜

hromycins, Esmolols, Estradiols, Estrogenics, Ethacrynic acids, Ethanolamines, Ethanols, Ethiodized oils, Etidronic acids, Etomidates, Famotidines, Fenoldopams, Fentanyls, Flumazenils, Fluoresceins, Fluphenazines, Folic acids, Fomepizoles, Fomivirsens, Fondaparinuxs, Foscarnets, Fosphenytoins, Furosemides, Gadoteridols, Gadoversetamides, Ganciclovirs, Gentamicins, Glucagons, Glucoses, Glycines, Glycopyrrolates, Gonadorelins, Gonadotropin chorionics, Haemophilus B polysaccharides, Hemins, Herbals, Histamines, Hydralazines, Hydrocortisones, Hydromorphones, Hydroxocobalamins, Hydroxyzines, Hyoscyamines, Ibutilides, Imiglucerases, Indigo carmines, Indomethacins, Iodides, Iopromides, Iothalamic acids, Ioxaglic acids, Ioxilans, Isoniazids, Isoproterenols, Japanese encephalitis vaccines, Kanamycins, Ketamines, Labetalols, Lepirudins, Levobupivacaines, Levothyroxines, Lincomycins, Liothyronines, Luteinizing hormones, Lyme disease vaccines, Mangafodipirs, Manthtols, Meningococcal polysaccharide vaccines, Meperidines, Mepivacaines, Mesoridazines, Metaraminols, Methadones, Methocarbamols, Methohexitals, Methyldopates, Methylergonovines, Metoclopramides, Metoprolols, Metronidazoles, Minocyclines, Mivacuriums, Morrhuic acids, Moxifloxacins, Muromonab-CD3s, Mycophenolate mofetils, Nafcillins, Nalbuphines, Nalmefenes, Naloxones, Neostigmines, Niacinamides, Nicardipines, Nitroglycerins, Nitroprussides, Norepinephrines, Orphenadrines, Oxacillins, Oxymorphones, Oxytetracyclines, Oxytocins, Pancuroniums, Panthenols, Pantothenic acids, Papaverines, Peginterferon alpha 2As, Penicillin Gs, Pentamidines, Pentazocines, Pentobarbitals, Perflutrens, Perphenazines, Phenobarbitals, Phentolamines, Phenylephrines, Phenytoins, Physostigmines, Phytonadiones, Polymyxin, Pralidoximes, Prilocaines, Procainamides, Procaines, Prochlorperazines, Progesterones, Propranolols, Pyridostigmine hydroxides, Pyridoxines, Quinidines, Quinupristins, Rabies immunoglobulins, Rabies vaccines, Ranitidines, Remifentanils, Riboflavins, Rifampins, Ropivacaines, Samariums, Scopolamines, Seleniums, Sermorelins, Sincalides, Somatrems, Spectinomycins, Streptokinases, Streptomycins, Succinylcholines, Sufentanils, Sulfamethoxazoles, Tacrolimuses, Terbutalines, Teriparatides, Testosterones, Tetanus antitoxins, Tetracaines, Tetradecyl sulfates, Theophyllines, Thiamines, Thiethylperazines, Thiopentals, Thyroid stimulating hormones, Tinzaparins, Tirofibans, Tobramycins, Tolazolines, Tolbutamides, Torsemides, Tranexamic acids, Treprostinils, Trifluoperazines, Trimethobenzamides, Trimethoprims, Tromethamines, Tuberculins, Typhoid vaccines, Urofollitropins, Urokinases, Valproic acids, Vasopressins, Vecuroniums, Verapamils, Voriconazoles, Warfarins, Yellow fever vaccines, Zidovudines, Zincs, Ziprasidone hydrochlorides, Aclacinomycins, Actinomycins, Adriamycins, Azaserines, 6-Azauridines, Carzinophilins, Chromomycins, Denopterins, 6 Diazo 50xo-L-Norleucines, Enocitabines, Floxuridines, Olivomycins, Pirarubicins, Piritrexims, Pteropterins, Tegafurs, Tubercidins, Alteplases, Arcitumomabs, bevacizumabs, Botulinum Toxin Type A'"'"'s, Botulinum Toxin Type B'"'"'s, Capromab Pendetides, Daclizumabs, Dornase alphas, Drotrecogin alphas, Imciromab Pentetates, Iodine-131'"'"'s, an antibiotic agent;

an angiogenesis inhibitor;

anti-cataract and anti-diabetic retinopathy substances;

carbonic anhydrase inhibitors;

mydriatics;

photodynamic therapy agents;

prostaglandin analogs;

growth factor;

anti-neoplastics;

anti-metabolites;

anti-viral;

amebicides and anti-protozoals;

anti-tuberculosis and anti-leprotic;

antitoxins and antivenins;

antihemophilic factor, anti-inhibitor coagulant complex, antithrombin III, coagulations Factor V, coagulation Factor IX, plasma protein fraction, von Willebrand factor;

antiplatelet agent a colony stimulating factor (CSF);

an erythropoiesis stimulator;

hemostatics and albumins;

Immune Globulins;

thrombin inhibitors;

anticoagulants;

a steroidal anti-inflammatory drug selected from among alclometasones, algestones, beclomethasones, betamethasones, budesonides, clobetasols, clobetasones, clocortolones, cloprednols, corticosterones, col˜

isones, cortivazols, deflazacorts, desonides, desoximetasones, dexamethasones, diflorasones, diflucortolones, difluprednates, enoxolones, fluazacorts, flucloronides, flumethasones, flunisolides, fluocinolones, fluocinonides, fluocortins, fluocortolones, fluorometholones, fluperolones, fluprednidenes, fluprednisolones, flurandrenolides, fluticasones, formocortals, halcinonides, halobetasols, halometasones, halopredones, hydrocortamates, hydrocortisones, loteprednol etabonate, mazipredones, medrysones, meprednisones, methylprednisolones, mometasone furoate, paramethasones, prednicarbates, prednisolones, prednisones, prednivals, prednylidenes, rimexolones, tixocortols and triamcinolones;

docosenoid, prostaglandins, prostaglandin analogs, antiprostaglandins and prostaglandin precursors;

miotics, cholinergics and anti-cholinesterase; and

anti-allergenics.

32. The pharmaceutical composition of claim 28, wherein the therapeutic agent is selected from the group consisting of an antibody, an Immune Globulin, a bisphosphonate, a cytokine, a chemotherapeutic agent, a coagulation factor and an insulin.

33. The pharmaceutical composition of claim 32, wherein the therapeutic agent is an insulin that is a fast-acting insulin.

34. The pharmaceutical composition of claim 33, wherein the fast-acting insulin is regular insulin or is an insulin analog.

35. The pharmaceutical composition of claim 34, wherein the fast-acting insulin is a regular insulin that is a human insulin or a pig insulin.

36. The pharmaceutical composition of claim 35, wherein:

the fast-acting insulin comprises an A chain having the sequence of amino acids set forth in SEQ ID NO;

862 and a B chain having the sequence of amino acids set forth in SEQ ID NO;

863;

or

the fast-acting insulin comprises an A chain with the sequence of amino acids set forth as amino acid residue positions 88-108 of SEQ ID NO;

864 and a B chain with the sequence of amino acids set forth as amino acid residue positions 25-54 of SEQ ID NO;

864.

37. The pharmaceutical composition of claim 34, wherein the fast-acting insulin is an insulin analog selected from among insulin lispro, insulin aspart and insulin glulisine.

38. The pharmaceutical composition of claim 37, wherein the insulin analog is an insulin having an A chain with the sequence of amino acids set forth in SEQ ID NO:

862 and a B chain having the sequence of amino acids set forth in any of SEQ NOS;

865-867.

39. The pharmaceutical composition of claim 33, wherein:

the amount of the modified PH20 polypeptide is from about 100 U/mL to 1000 U/mL; and

the amount of the fast-acting insulin is from about 10 U/mL to 1000 U/mL.

40. The pharmaceutical composition of claim 39, further comprising:

a pH from about 7.0 to 7.6;

NaCl at a concentration from about 0.1 mM to 200 mM; and

an anti-microbially effective amount of one or more preservatives, wherein at least one preservative is a phenolic preservative.

41. The pharmaceutical composition of claim 40, wherein the anti-microbially effective amount is a total amount of one or more preservative agents as a percentage (%) of mass concentration (w/v) that is between 0.05% to 0.6%, inclusive.

42. The pharmaceutical composition of claim 40, wherein the preservative(s) is(are) selected from the group consisting of phenol, metacresol (m-cresol), benzyl alcohol, and a paraben.

43. The pharmaceutical composition of claim 40, further comprising:

a surfactant in an amount as a percentage (%) of mass concentration (w/v) in the formulation composition that is at least or at least about 0.001%;

a buffering agent that is a non-metal binding agent or is a metal binding agent, wherein the concentration of the buffering agent is between about 1 mM to 100 mM;

glycerin in a concentration less than 60 mM;

an antioxidant at a concentration between about 2 mM to 50 mM; and

/or zinc at a concentration of between about 0.001 to 0.1 mg per 100 units of insulin (mg/100 U).

44. The pharmaceutical composition of claim 43, wherein the surfactant is selected from the group consisting of a polypropylene glycol, polyethylene glycol, glycerin, sorbitol, poloxamer and polysorbate.

45. The pharmaceutical composition of claim 43, wherein the buffering agent is selected from the group consisting of Tris, histidine, phosphate and citrate.

46. The pharmaceutical composition of claim 43, wherein the antioxidant is selected from the group consisting of cysteine, tryptophan and methionine.

47. A closed loop system, comprising the pharmaceutical composition of claim 33.

48. An insulin pump, comprising the pharmaceutical composition of claim 33.

49. An insulin pen, comprising the pharmaceutical composition of claim 33.