Mass spectrometry with selective ion filtration by digital thresholding

• US 9,449,802 B2
• Filed: 03/23/2015
• Issued: 09/20/2016
• Est. Priority Date: 05/24/2004
• Status: Active Grant

• Current Assignee: Ibis Biosciences Incorporated
• Original Assignee: Ibis Biosciences Incorporated
• Inventors: Hofstadler, Steven A., Drader, Jared J., Schink, Amy L.
• Primary Examiner(s): Vanore, David A
• Application Number:
  US14/665,921
• Publication Number:
  US 20160071710A1
• Time in Patent Office: 547 Days
• Field of Search: 250/282
• US Class Current: 1/1
• CPC Class Codes: G01N 33/6848 Methods of protein analysis involving mass spectrometry
• CPC Class Codes: H01J 49/0009 Calibration of the apparatus
• CPC Class Codes: H01J 49/0027 Methods for using particle spectrometers
• CPC Class Codes: H01J 49/0036 Step by step routines describing the handling of the data generated during a measurement
• CPC Class Codes: H01J 49/02 Details
• CPC Class Codes: H01J 49/165 Electrospray ionisation
• CPC Class Codes: H01J 49/26 Mass spectrometers or separator tubes
• CPC Class Codes: H01J 49/40 Time-of-flight spectrometers H01J49/36 takes precedence

Abstract

The methods described herein generally relate to characterization of large analytes, such as biomolecules, by molecular mass analysis. Specifically, the methods are directed to molecular mass analysis of singly- or multiply-charged ions by selective ion filtering carried out by a digital thresholding process.

Claims

1. A method of identifying a multiply-charged ion comprising:

a) specifying a digital signal threshold on a ion identification apparatus, wherein the ion identification apparatus comprises;

i) an ion detector;

ii) a digitizer for converting an analog signal to a digital signal;

iii) an analog signal transfer means for transferring an analog signal from the detector to the digitizer; and

iv) a digital threshold filter, for setting a digital signal threshold, in digital data communication with the digitizer, in response to a digital signal input from the digitizer, independently outputting a digital signal to a data file only if the digital signal input is greater than the specified digital signal threshold;

wherein upon a ion identification apparatus measurement of the multiply-charged ion, the filtered digital signal output to the data file originates from the detection of the multiply-charged ion and excludes digital signal output from analog signals arising from singly-charged ions.

2. The method of claim 1, wherein said digitizer converts a plurality of analog signals to a plurality of digital signals, and wherein said plurality of digital signals from said digitizer are split into multiple parallel data streams each of which is subjected to a different digital signal threshold.

3. The method of claim 2, wherein a plurality of unique digital signal thresholds are specified to obtain parallel differentially filtered data streams stored in corresponding data files.

4. The method of claim 2, wherein one or more of said parallel data streams subjected to different signal thresholds are subtracted from one or more different parallel data streams subjected to different signal thresholds.

5. The method of claim 2, wherein one or more of said plurality of analog signals are obtained from chemical, protease, or restriction digestion of proteins or nucleic acids.

6. The method of claim 2, wherein one or more of said plurality of analog signals are obtained from a biomolecule having a non-covalently-bound small molecule.

7. The method of claim 2, wherein one or more of said plurality of analog signals are obtained from one or more internal calibrants.

8. The method of claim 2, wherein data acquisition is controlled by data acquisition software and post-processing of data is performed by post-processing software.

9. The method of claim 1, wherein said singly charged ions comprise ampholytes used in capillary isoelectric focusing, phosphate components of buffers used in capillary zone electrophoresis, solution matrix modifiers used to promote micelle formation in micellar electrokinetic chromatography, buffer impurities, plastic ware, and enzyme stabilizers.

10. The method of claim 1, wherein said ion identification apparatus is a mass spectrometer.