Microfluidic cartridge for processing and detecting nucleic acids
First Claim
1. A cartridge for processing a sample, the cartridge comprising:
- a first layer comprising a sample port;
an intermediate substrate that is coupled to the first layer, partially separated from the first layer by a film layer, and configured to form a sealed waste chamber and a waste port into the sealed waste chamber, wherein the sealed waste chamber directly opposes the first layer across the film layer and defines an external void; and
a fluidic pathway, superior to the intermediate substrate and at least partially separated from the sealed waste chamber by a deformable layer, wherein the fluidic pathway is fluidly coupled to the sample port, and wherein, upon deformation of the deformable layer through the external void defined by the waste chamber, the fluidic pathway is occluded and configured to transfer waste fluid of the sample through the waste port into an interior portion of the sealed waste chamber.
4 Assignments
0 Petitions
Accused Products
Abstract
A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.
138 Citations
20 Claims
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1. A cartridge for processing a sample, the cartridge comprising:
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a first layer comprising a sample port; an intermediate substrate that is coupled to the first layer, partially separated from the first layer by a film layer, and configured to form a sealed waste chamber and a waste port into the sealed waste chamber, wherein the sealed waste chamber directly opposes the first layer across the film layer and defines an external void; and a fluidic pathway, superior to the intermediate substrate and at least partially separated from the sealed waste chamber by a deformable layer, wherein the fluidic pathway is fluidly coupled to the sample port, and wherein, upon deformation of the deformable layer through the external void defined by the waste chamber, the fluidic pathway is occluded and configured to transfer waste fluid of the sample through the waste port into an interior portion of the sealed waste chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A cartridge for processing of a set of samples, the cartridge comprising:
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a first layer and an intermediate substrate coupled to the first layer and partially separated from the first layer by a film layer, wherein the intermediate substrate forms a sealed waste chamber and a set of waste ports into the sealed waste chamber, wherein the sealed waste chamber directly opposes the first layer across the film layer and defines an external void; a first fluidic pathway, formed by at least a portion of the first layer; and a second fluidic pathway in parallel with the first fluidic pathway and formed by at least a portion of the first layer, wherein the first fluidic pathway and the second fluidic pathway are each at least partially separated from the sealed waste chamber by a deformable layer that is deformable through the external void, and each of the first and the second fluidic pathways is configured to transfer waste fluid of the set of samples into the sealed waste chamber through the set of waste ports. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification