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Methods of preparing and using novel stem cell compositions and kits comprising the same

DC CAFC
  • US 9,453,202 B2
  • Filed: 10/07/2004
  • Issued: 09/27/2016
  • Est. Priority Date: 10/08/2003
  • Status: Active Grant
First Claim
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1. A method of treating inflammation at a site of a musculoskeletal injury or disease in a mammal, comprising:

  • (a) preparing a cell population comprising adipose tissue-derived stem cells for introducing into a mammal by a method comprising;

    (i) processing adipose tissue obtained from the mammal to release cells therein, wherein the adipose tissue comprises stem cells, wherein said processing comprises one or more procedures selected from the group consisting of;

    physical cutting or mincing of the adipose tissue;

    treating the adipose tissue with an enzyme that facilitates the release of cells;

    exposing the adipose tissue to ultrasonic energy; and

    treating the adipose tissue with perfluorocarbons, 

    and wherein said processing releases cells and a fat layer; and

    (ii) separating the cells released in (i) from the fat layer, wherein said separating comprises one or more procedures selected from the group consisting of;

    use of a density gradient;

    centrifugation; and

    filtration;

    thereby preparing the cell population comprising adipose tissue-derived stem cells; and

    (b) providing the cell population of (a)(ii) directly to the site of the musculoskeletal injury or disease in the mammal to treat the inflammation at the site of the musculoskeletal injury or disease,wherein said method does not include isolating stem cells separated in (a)(ii) from other cells separated in (a)(ii).

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