Methods of preparing and using novel stem cell compositions and kits comprising the same
DC CAFCFirst Claim
1. A method of treating inflammation at a site of a musculoskeletal injury or disease in a mammal, comprising:
- (a) preparing a cell population comprising adipose tissue-derived stem cells for introducing into a mammal by a method comprising;
(i) processing adipose tissue obtained from the mammal to release cells therein, wherein the adipose tissue comprises stem cells, wherein said processing comprises one or more procedures selected from the group consisting of;
physical cutting or mincing of the adipose tissue;
treating the adipose tissue with an enzyme that facilitates the release of cells;
exposing the adipose tissue to ultrasonic energy; and
treating the adipose tissue with perfluorocarbons,
and wherein said processing releases cells and a fat layer; and
(ii) separating the cells released in (i) from the fat layer, wherein said separating comprises one or more procedures selected from the group consisting of;
use of a density gradient;
centrifugation; and
filtration;
thereby preparing the cell population comprising adipose tissue-derived stem cells; and
(b) providing the cell population of (a)(ii) directly to the site of the musculoskeletal injury or disease in the mammal to treat the inflammation at the site of the musculoskeletal injury or disease,wherein said method does not include isolating stem cells separated in (a)(ii) from other cells separated in (a)(ii).
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Abstract
The present invention provides novel stem cell compositions having significant therapeutic and practical advantages, as well as methods of preparing and using such compositions for the treatment and prevention of injury and disease in patients. The invention may be applied to stem cell populations isolated from a wide variety of animals, including humans, and tissues. In particular applications, the invention is used to prepare a stem cell composition from a collagen-based tissue, such as adipose tissue, isolated from a patient, and the stem cell composition is subsequently provided to a site of actual or potential injury in the patient. The invention further includes related kits comprising the stem cell compositions, which are remarkably stable and retain viability and efficacy during storage and shipment.
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Citations
19 Claims
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1. A method of treating inflammation at a site of a musculoskeletal injury or disease in a mammal, comprising:
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(a) preparing a cell population comprising adipose tissue-derived stem cells for introducing into a mammal by a method comprising; (i) processing adipose tissue obtained from the mammal to release cells therein, wherein the adipose tissue comprises stem cells, wherein said processing comprises one or more procedures selected from the group consisting of; physical cutting or mincing of the adipose tissue; treating the adipose tissue with an enzyme that facilitates the release of cells; exposing the adipose tissue to ultrasonic energy; and treating the adipose tissue with perfluorocarbons,
and wherein said processing releases cells and a fat layer; and(ii) separating the cells released in (i) from the fat layer, wherein said separating comprises one or more procedures selected from the group consisting of; use of a density gradient; centrifugation; and filtration; thereby preparing the cell population comprising adipose tissue-derived stem cells; and (b) providing the cell population of (a)(ii) directly to the site of the musculoskeletal injury or disease in the mammal to treat the inflammation at the site of the musculoskeletal injury or disease, wherein said method does not include isolating stem cells separated in (a)(ii) from other cells separated in (a)(ii). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification