Pasteurisation process for microbial cells and microbial oil

• US 9,457,108 B2
• Filed: 06/23/2014
• Issued: 10/04/2016
• Est. Priority Date: 06/19/2002
• Status: Expired due to Term

• Current Assignee: DSM IP Assets BV (DSM-Firmenich AG), DSM Services USA, Inc
• Original Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Inventors: Schaap, Albert, Verkoeijen, Daniel
• Primary Examiner(s): NAVARRO, ALBERT MARK
• Application Number:
  US14/311,461
• Publication Number:
  US 20140316151A1
• Time in Patent Office: 834 Days
• US Class Current: 1/1
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23D 9/02 characterised by the production or working-up
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23L 33/115 Fatty acids or derivatives thereof; Fats or oils
• CPC Class Codes: A23L 33/12 Fatty acids or derivatives thereof
• CPC Class Codes: A61K 2800/85 Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
• CPC Class Codes: A61K 8/67 Vitamins
• CPC Class Codes: A61K 8/925 of animal origin
• CPC Class Codes: A61K 8/9706 Algae
• CPC Class Codes: A61K 8/9728 Fungi, e.g. yeasts
• CPC Class Codes: A61L 2/04 Heat A61L2/08 takes precedence
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: C07C 57/03 Monocarboxylic acids
• CPC Class Codes: C11B 1/10 by extracting
• CPC Class Codes: C12N 1/005 after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
• CPC Class Codes: C12P 7/6427 Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
• CPC Class Codes: C12P 7/6432 Eicosapentaenoic acids [EPA]
• CPC Class Codes: C12P 7/6434 Docosahexenoic acids [DHA]
• CPC Class Codes: C12P 7/6463 : obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/6472 : containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

A pasteurization protocol for pasteurizing microbial cells is disclosed. The protocol has a heating stage, a plateau stage at which the cells are held at a (maximum and) constant temperature, and a cooling stage. The heating and cooling stages are rapid, the temperature of the cells passing through 40 to 80° C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5° C./minute and during cooling is at least −0.5° C./minute. The plateau maximum temperature is from 70 to 85° C. By plotting the pasteurization protocol on a time (t, minutes) versus temperature (T, ° C.), one obtains a trapezium having an area less than 13,000° C. minute. This results in a smaller energy input (so a reduction in costs) and a better quality oil having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.

Claims

1. A microbial oil comprising docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and mixtures thereof having a triglyceride content of at least 90%;

a peroxide value (POV) of less than 1.5, or optionally less than 1.0; and

/or an anisidine value (AnV) of less than 15, or optionally less than 12.

2. The microbial oil of claim 1 wherein the oil comprises at least 40% DHA, EPA, and mixtures thereof and/or the oil is a crude or unrefined oil.

3. The microbial oil of claim 1, wherein the DHA, EPA, and mixtures thereof are produced from microbial cells, wherein the microbial cells are selected from Cryptochodinium, Thraustochytrium, and mixtures thereof.

4. The microbial oil of claim 3, wherein the microbial cells are Cryptochodinium.

5. The microbial oil of claim 4, wherein the microbial cells are Cryptochodinium Cohnii.

6. The microbial oil of claim 3, wherein the DHA, EPA and mixtures thereof are produced from microbial cells, wherein the microbial cells are Thraustochytrium.

7. A foodstuff comprising the microbial oil of claim 2.

8. The foodstuff of claim 7, which is an animal feed or a marine feed.

9. The microbial oil of claim 2, wherein the microbial oil is subjected to one or more refining steps to provide a refined oil, wherein said one or more refining steps are selected from the group consisting of acid treatment or degumming, alkali treatment or free fatty acid removal, bleaching or pigment removal, filtration, winterization, deodorizing and polishing.

10. A foodstuff comprising the refined oil of claim 9.

11. The foodstuff of claim 10, which is an infant formula.

23. A foodstuff comprising the microbial oil of claim 1.

24. The foodstuff of claim 23, which is an animal feed or a marine feed.

12. A microbial oil comprising DHA, EPA, and mixtures thereof having a triglyceride content of at least 50%;

a peroxide value (POV) of less than 1.5, or optionally less than 1.0; and

/or an anisidine value (AnV) of less than 15, or optionally less than 12.

13. The microbial oil of claim 12 wherein the oil comprises at least 40% DHA, EPA, and mixtures thereof and/or the oil is a crude or unrefined oil.

14. A foodstuff comprising the microbial oil of claim 13.

15. The foodstuff of claim 14, which is an animal feed or a marine feed.

16. The microbial oil of claim 13, wherein the microbial oil is subjected to one or more refining steps to provide a refined oil, wherein said one or more refining steps are selected from the group consisting of acid treatment or degumming, alkali treatment or free fatty acid removal, bleaching or pigment removal, filtration, winterization, deodorizing and polishing.

17. A foodstuff comprising the refined oil of claim 16.

18. The foodstuff of claim 17, which is an infant formula.

19. The microbial oil of claim 12, wherein the DHA, EPA and mixtures thereof are produced from microbial cells, wherein the microbial cells are selected from Cryptochodinium, Thraustochytrium, and mixtures thereof.

20. The microbial oil of claim 19, wherein the microbial cells are Cryptochodinium.

21. The microbial oil of claim 20, wherein the microbial cells are Cryptochodinium Cohnii.

22. The microbial oil of claim 12, wherein the DHA, EPA and mixtures thereof are produced from microbial cells, wherein the microbial cells are Thraustochytrium.

25. The microbial oil of claim 12, wherein the microbial oil is subjected to one or more refining steps to provide a refined oil, wherein said one or more refining steps are selected from the group consisting of acid treatment or degumming, alkali treatment or free fatty acid removal, bleaching or pigment removal, filtration, winterization, deodorizing and polishing.

26. A foodstuff comprising the refined oil of claim 25.

27. The foodstuff of claim 26, which is an infant formula.