Differentiated human embryoid cells and a method for producing them

• US 9,458,425 B1
• Filed: 05/12/2000
• Issued: 10/04/2016
• Est. Priority Date: 05/14/1999
• Status: Expired due to Fees

• Current Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem), TECHNION RESEARCH AND DEVELOPMENT FOUNDATION, LTD.
• Original Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem), TECHNION RESEARCH AND DEVELOPMENT FOUNDATION, LTD.
• Inventors: Itskovitz-Eldor, Joseph, Benvenisty, Nissim
• Primary Examiner(s): Ton, Thaian N
• Application Number:
  US09/979,001
• Time in Patent Office: 5,989 Days
• Field of Search: 435/325, 435/363, 435/366, 435/374, 435/377, 435/404, 435/405, 435/395, 435/401
• US Class Current: 1/1
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 5/0603 Embryonic cells production of embryos, nuclear transfer A01K67/027; Embryoid bodies
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12N 5/0657 Cardiomyocytes; Heart cells

Abstract

A process for obtaining human derived embryoid bodies (hEB). Human embryonic stem cells are incubated in vitro in a liquid growth medium under conditions in which the cells undergo differentiation, but do not adhere to the walls of the container. The invention also provides hEBs obtained by the process.

Claims

1. A process for obtaining at least one human-derived embryoid body (hEB), comprising:

(a) providing human embryonic stem (hES) cells;

(b) growing the hES cells in vitro suspended in a liquid growth medium in a vessel under conditions under which said cells undergo differentiation and said cells or aggregates thereof do not adhere to the vessel wall, wherein said conditions comprise culturing cells in a vessel having walls of a kind to which cells do not adhere; and

(c) incubating for a time sufficient to develop hEBs from said cells.