Methods of hydrolyzing pretreated densified biomass particulates and systems related thereto

• US 9,458,482 B2
• Filed: 12/22/2014
• Issued: 10/04/2016
• Est. Priority Date: 08/24/2009
• Status: Active Grant

• Current Assignee: The Board of Trustees of Michigan State University (Michigan State University), The Michigan Biotechnology Institute
• Original Assignee: The Board of Trustees of Michigan State University (Michigan State University), The Michigan Biotechnology Institute
• Inventors: Bals, Bryan, Teymouri, Farzaneh, Campbell, Timothy J., Dale, Bruce E.
• Primary Examiner(s): Toomer, Cephia D
• Application Number:
  US14/579,377
• Publication Number:
  US 20150112101A1
• Time in Patent Office: 652 Days
• US Class Current: 1/1
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 2201/00 Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12P 7/14 Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y02E 50/30 Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

A method is provided in which pretreated and densified cellulosic biomass particulates can be hydrolyzed at a high solids loading rate as compared with the solids loading rate of loose hydrolysable cellulosic biomass fibers. The resulting high concentration sugar-containing stream can be easily converted to biofuels or to an entire suite of other useful bioproducts.

Claims

1. A method comprising:

enzymatically hydrolyzing one or more hydrolysable densified biomass particulates to produce a convertible sugar-containing stream, wherein said densified biomass particulates are produced by;

subjecting a quantity of plant biomass fibers containing lignin and/or hemicellulose to a pretreatment to cause at least a portion of the lignin and/or hemicellulose in said biomass fibers to move to an outer surface to produce a quantity of pretreated tacky plant biomass fibers; and

densifying said pretreated tacky plant biomass fibers to produce the one or more hydrolysable densified biomass particulates, wherein said densified plant biomass fibers are densified without using added binder.

2. The method of claim 1 wherein the solids loading is about 12% to about 35%, or from about 12% to about 20%, or from about 18% to about 24%.

3. The method of claim 1 wherein said biomass fibers are corn stover fibers, switchgrass fibers, wood fibers, prairie cord grass fibers, or combinations thereof.

4. The method of claim 1 wherein the pretreatment and the densifying steps are performed as an integrated process at a single location.

5. The method of claim 1 wherein the pretreatment is an ammonia pretreatment or a sodium hydroxide pretreatment.

6. The method of claim 5 wherein the ammonia pretreatment is liquid ammonia fiber expansion (AFEX) pretreatment or a gaseous AFEX pretreatment.

7. The method of claim 5 wherein the method further comprises adding water and/or steam to said biomass fibers prior to and/or during the pretreatment.

8. The method of claim 1 wherein the convertible sugar-containing stream is fermented to produce a bioproduct.

9. The method of claim 8 wherein the bioproduct is a biofuel.

10. A product produced according to the method of claim 1.

18. The method of claim 1 wherein sugar in the convertible sugar-containing stream is produced from said densified biomass particulates at a faster rate as compared to a sugar-containing stream produced using non-densified biomass.

19. The product of claim 10 wherein the fermentable sugar-containing stream contains glucose, xylose, mannose, arabinose, or a combination thereof.

22. The method of claim 1 wherein the hydrolyzing is performed in a tank having an impeller located therein, wherein an impeller to tank diameter ratio is from 1:

4 to 3;

4.

23. The method of claim 6 wherein the ammonia pretreatment is performed in a pretreatment vessel and the method further comprises removing residual ammonia remaining after the subjecting step by introducing steam into the pretreatment vessel.

27. The method of claim 1 wherein the subjecting step takes place in a stirred reactor.

28. The method of claim 27 wherein the stirred reactor is a vertical stirred reactor.

11. A system comprising:

a hydrolyzing facility for enzymatically hydrolyzing one or more hydrolysable densified biomass particulates to produce a convertible sugar-containing stream, wherein said densified biomass particulates are produced by;

subjecting a quantity of plant biomass fibers containing lignin and/or hemicellulose to a pretreatment to cause at least a portion of the lignin and/or hemicellulose in said biomass fibers to move to an outer surface to produce a quantity of pretreated tacky plant biomass fibers; and

densifying said pretreated tacky plant biomass fibers to produce the one or more hydrolysable densified biomass particulates wherein said densified plant biomass fibers are densified without using added binder.

12. The system of claim 11 wherein the hydrolyzing facility is part of a bioproduct production facility, and the convertible sugar-containing stream is fermented in the bioproduct production facility to produce a bioproduct.

13. The system of claim 12 wherein the bioproduct production facility is an ethanol production facility and/or said biomass fibers are corn stover fibers.

14. The system of claim 11 further comprising:

a pretreatment facility for the subjecting step; and

a densifying facility for the densifying step.

15. The system of claim 11 wherein the pretreatment is an ammonia pretreatment or a sodium hydroxide pretreatment.

16. The system of claim 15 wherein the ammonia pretreatment is liquid ammonia fiber expansion (AFEX) pretreatment or a gaseous AFEX pretreatment.

17. The system of claim 14 wherein the pretreatment facility and densifying facility are co-located.

24. The system of claim 11 wherein the hydrolyzing is performed in a tank having an impeller located therein, wherein an impeller to tank diameter ratio is from 1:

4 to 3;

4.

25. The system of claim 14 wherein the pretreatment facility includes an apparatus for adding steam and/or water to the plant biomass fibers prior to and/or during the pretreatment.

26. The system of claim 16 wherein the ammonia pretreatment is performed in a pretreatment vessel and residual ammonia is removed after the subjecting step by introducing steam into the pretreatment vessel.

29. The system of claim 11 wherein the subjecting step takes place in a stirred reactor.

30. The system of claim 29 wherein the stirred reactor is a vertical stirred reactor.

20. A method comprising:

subjecting a quantity of plant biomass fibers containing lignin and/or hemicellulose to a pretreatment to cause at least a portion of the lignin and/or hemicellulose in said biomass to move to an outer surface to produce a quantity of pretreated tacky plant biomass fibers;

densifying said pretreated tacky plant biomass fibers to produce the one or more hydrolysable densified biomass particulates wherein said biomass fibers is densified without using added binder; and

enzymatically hydrolyzing the one or more hydrolysable densified biomass particulates to produce a convertible sugar-containing stream, wherein, the subjecting, densifying and enzymatically hydrolyzing steps are performed as an integrated process at a single location.

21. A method comprising:

providing a hydrolysable densified biomass particulate or a plurality thereof, the hydrolysable densified biomass particulate or particulates each comprising a plurality of plant biomass fibers containing lignin and/or hemicellulose at an outer surface of the fibers, wherein the hydrolysable densified biomass particulate or particulates are in a compressed particulate form and are free from added binder; and

hydrolyzing the hydrolysable densified biomass particulate or plurality thereof to produce a convertible sugar-containing stream.