Point of care sensor systems
First Claim
1. A cartridge for sensing one or more analytes in a sample, the cartridge comprising:
- a sample inlet chamber for receiving a sample;
a reagent chamber configured to store a solid phase reagent;
a mixing circuit connected to the sample inlet chamber and comprising a mixing chamber and the reagent chamber;
one or more sensors; and
a detection channel providing a flow path over the one or more sensors, wherein the cartridge includes at least two sensors and is configured to perform non-capture electrochemical enzymatic sensing and electrochemical enzyme-linked immunosorbent assay (ELISA) sensing on a single sample.
2 Assignments
0 Petitions
Accused Products
Abstract
Provided are point of care sensor systems that include portable readers and disposable cartridges for receiving and analyzing samples. A cartridge may be equipped with one or more sensor channels, each containing one or more sensors. After providing a sample to a cartridge, the cartridge can be inserted into a reader, which can interact with the cartridge to perform on-cartridge sensing and receive signals indicating the presence and/or quantity of one or more targets in the sample. Examples of cartridges can include cardiac panels, sepsis panels and the like. In some embodiments, the same sensor hardware may be configured for multiple measurements of different targets conducted at different time frames. Also provided herein are novel on-cartridge solid and liquid reagent storage and delivery mechanisms.
18 Citations
65 Claims
-
1. A cartridge for sensing one or more analytes in a sample, the cartridge comprising:
-
a sample inlet chamber for receiving a sample; a reagent chamber configured to store a solid phase reagent; a mixing circuit connected to the sample inlet chamber and comprising a mixing chamber and the reagent chamber; one or more sensors; and a detection channel providing a flow path over the one or more sensors, wherein the cartridge includes at least two sensors and is configured to perform non-capture electrochemical enzymatic sensing and electrochemical enzyme-linked immunosorbent assay (ELISA) sensing on a single sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 63, 64, 65)
-
-
21. A cartridge for sensing first and second target analytes in a biological sample, comprising:
a first working electrode coated with one or more enzymes configured to react with a first target analyte to directly or indirectly produce a first electrochemical signal; and a second working electrode having capture species attached thereto, the capture species configured to capture the second target analyte to directly or indirectly produce a second electrochemical signal, wherein the cartridge is a single sample, single use, disposable cartridge. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
-
38. A reader configured to detect bubbles on a sensor of a cartridge comprising:
-
an interface for receiving the cartridge; and computer readable media including instructions to; measure the impedance of a sensor well on the cartridge; determine the series resistance Rs and the series capacitance Cs of an equivalent circuit; determine if a relationship between series resistance Rs and the series capacitance Cs meets a specified criteria; and based on the determination of the relationship, determine whether a bubble is detected. - View Dependent Claims (39)
-
-
40. A cartridge for sensing one or more analytes in a sample, the cartridge comprising:
-
a sample inlet chamber for receiving a sample; a reagent chamber configured to store a solid phase reagent; a mixing circuit connected to the sample inlet chamber and comprising a mixing chamber and the reagent chamber; one or more sensors; and a detection channel providing a flow path over the one or more sensors, wherein the cartridge is configured to receive only pneumatic and electric inputs from a reader. - View Dependent Claims (41, 42, 43, 44, 45, 46, 47)
-
-
48. A cartridge for sensing one or more analytes in a sample, the cartridge comprising:
-
a sample inlet chamber for receiving a sample; a reagent chamber configured to store a solid phase reagent; one or more sensors; a detection channel providing a flow path over the one or more sensors; a de-bubbling channel disposed between the reagent chamber and the detection channel and configured to vent out bubbles in the sample; and a mixing circuit connected to the sample inlet chamber and comprising a mixing chamber, the de-bubbling channel, and the reagent chamber.
-
-
49. A cartridge for sensing one or more analytes in a sample, the cartridge comprising:
-
a sample inlet chamber for receiving a sample; a plasma filtration membrane; a vacuum line connected to the plasma filtration membrane; a reagent chamber configured to store a solid phase reagent; a mixing circuit connected to the sample inlet chamber and comprising a mixing chamber and the reagent chamber; one or more sensors; and a detection channel providing a flow path over the one or more sensors. - View Dependent Claims (50, 51, 52, 53, 54, 55, 56, 57, 58, 59)
-
-
60. A cartridge for sensing one or more analytes in a sample, the cartridge comprising:
-
a sample inlet chamber for receiving a sample; a reagent chamber configured to store a solid phase reagent; a mixing circuit connected to the sample inlet chamber and comprising a mixing chamber and the reagent chamber; one or more sensors; a detection channel providing a flow path over the one or more sensors; and a de-bubbling channel disposed between the reagent chamber and the detection channel and configured to vent out bubbles in the sample, wherein the de-bubbling channel is covered by a hydrophobic membrane. - View Dependent Claims (61)
-
-
62. A cartridge for sensing one or more analytes in a sample, the cartridge comprising:
-
a sample inlet chamber for receiving a sample; a reagent chamber configured to store a solid phase reagent; a mixing circuit connected to the sample inlet chamber and comprising a mixing chamber and the reagent chamber; one or more sensors; a detection channel providing a flow path over the one or more sensors; and a plurality of fluid stops connected to a hydrophobic membrane.
-
Specification