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Method of determining the nucleotide sequence of oligonucleotides and DNA molecules

  • US 9,458,500 B2
  • Filed: 06/08/2013
  • Issued: 10/04/2016
  • Est. Priority Date: 05/01/1998
  • Status: Expired due to Fees
First Claim
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1. An apparatus for DNA sequencing comprising:

  • a) at least one reaction chamber including a DNA primer/template system which produces a detectable reaction event when a DNA polymerase enzyme incorporates an unlabeled and unblocked deoxyribonucleotide monophosphate onto the 3′

    end of the primer strand;

    b) a component for introducing into, and evacuating from, said reaction chamber at least one reagent selected from the group consisting of;

    buffers, electrolytes, DNA template, DNA primer, unlabeled and unblocked deoxyribonucleotides, and polymerase enzymes;

    c) a system for converting said detectable reaction event into an electrical signal based on an electrical potential generated from said detectable reaction event in the at least one reaction chamber; and

    d) a voltmeter configured to measure said electrical signal in the at least one reaction chamber.

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