Apparatus for selective excitation of microparticles
First Claim
1. An apparatus for optical excitation of a target, the apparatus comprising:
- a laser to generate a first laser beam;
a first interference pattern generation module receiving the first laser beam, the first interference pattern generation module comprising;
a beam splitter for splitting the first laser beam into a second laser beam and a third laser beam;
a window, the third laser beam passing through the window, the window modulating an optical phase of the third laser beam with respect to the second laser beam; and
a galvanometer coupled to the window to rotate the window,wherein the first interference pattern generation module is configured to generate an excitation pattern for selectively exciting the target by interference between the second laser beam and the third laser beam.
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Abstract
Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more reagents, selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an interference pattern generation module that splits a first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams.
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Citations
11 Claims
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1. An apparatus for optical excitation of a target, the apparatus comprising:
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a laser to generate a first laser beam; a first interference pattern generation module receiving the first laser beam, the first interference pattern generation module comprising; a beam splitter for splitting the first laser beam into a second laser beam and a third laser beam; a window, the third laser beam passing through the window, the window modulating an optical phase of the third laser beam with respect to the second laser beam; and a galvanometer coupled to the window to rotate the window, wherein the first interference pattern generation module is configured to generate an excitation pattern for selectively exciting the target by interference between the second laser beam and the third laser beam. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A system for sequencing nucleic acid microparticles, the system comprising:
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a sequence reaction module configured to apply one or more sequencing reagents to the nucleic acid microparticles; a selective microparticle excitation module configured to selectively excite the nucleic acid microparticles in an excitation pattern generated by an interference of a plurality of illumination beams onto the nucleic acid microparticles; an optical imaging module configured to generate one or more optical images of the nucleic acid microparticles at a resolution insufficient to resolve individual microparticles; and an image processing module configured to process the optical images of the nucleic acid microparticles using information on the excitation pattern and to determine presence or absence of an optical signature, the presence or absence of the optical signature indicating the sequence information of the nucleic acid, wherein the selective microparticle excitation module comprises; a laser to generate a first laser beam; a first interference pattern generation module receiving the first laser beam, the first interference pattern generation module comprising; a beam splitter for splitting the first laser beam into a second laser beam and a third laser beam; a window, the third laser beam passing through the window, the window modulating an optical phase of the third laser beam with respect to the second laser beam; and a galvanometer coupled to the window to rotate the window, wherein the first interference pattern generation module is configured to generate an excitation pattern for selectively exciting the target by interference between the second laser beam and the third laser beam. - View Dependent Claims (8, 9, 10, 11)
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Specification