Compositions and methods for nucleic acid delivery
First Claim
1. A structure for localized and controlled release of nucleic acid agents comprising:
- a bandage substrate; and
a multilayer film coated on the substrate, wherein adjacent layers of the multilayer film are associated with one another via one or more non-covalent interactions,wherein the multilayer film includes;
a first bilayer portion; and
a second bilayer portion adjacent to the first bilayer portion, wherein the second bilayer portion is disposed on the bandage substrate, andwherein;
the first bilayer portion includes at least fifty bilayers, each bilayer of the first bilayer portion comprising a first layer comprising an siRNA agent, and a second layer comprising chitosan,the second bilayer portion includes at least ten bilayers, each bilayer of the second bilayer portion comprising a first layer comprising dextran sulfate and a second layer comprising a poly(β
-amino ester) having the following structural formula;
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides, among other things, multilayer film coating compositions, coated substrates and methods thereof. In some embodiments, a structure includes a substrate; and a multilayer film coated on the substrate, wherein adjacent layers of the multilayer film are associated with one another via one or more non-covalent interactions, wherein the multilayer film comprises a first nucleic acid agent present at a loading density, and further wherein the multilayer film is characterized in that, when the structure is placed on a subject so that the multilayer film contacts cells, the first nucleic acid agent is released with a profile characterized by a feature selected from the group consisting of 1) being a burst-free release; 2) being a sustained release; and 3) exhibiting in vitro and/or in vivo biological effectiveness.
142 Citations
11 Claims
-
1. A structure for localized and controlled release of nucleic acid agents comprising:
-
a bandage substrate; and a multilayer film coated on the substrate, wherein adjacent layers of the multilayer film are associated with one another via one or more non-covalent interactions, wherein the multilayer film includes; a first bilayer portion; and a second bilayer portion adjacent to the first bilayer portion, wherein the second bilayer portion is disposed on the bandage substrate, and wherein; the first bilayer portion includes at least fifty bilayers, each bilayer of the first bilayer portion comprising a first layer comprising an siRNA agent, and a second layer comprising chitosan, the second bilayer portion includes at least ten bilayers, each bilayer of the second bilayer portion comprising a first layer comprising dextran sulfate and a second layer comprising a poly(β
-amino ester) having the following structural formula; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
Specification