×

Compositions and methods for oxygenation of nucleic acids containing 5-methylpyrimidine

  • US 9,464,277 B2
  • Filed: 04/20/2015
  • Issued: 10/11/2016
  • Est. Priority Date: 03/15/2012
  • Status: Active Grant
First Claim
Patent Images

1. A method for differentiating a 5-methylcytosine (5-mC) from 5-hydroxymethylcytosine (5-hmC) in a genome or genome fragment, comprising:

  • (a) reacting the isolated genome or genome fragment containing 5-mC and 5-hmC with;

    i. a UDP-associated sugar and a glucosyltransferase that transfers the sugar to the 5hmC, andii. a polypeptide that has 5-methylpyrimidine oxygenase activity and comprises an amino acid sequence that is at least 90% identical to amino acids 154-304 of SEQ ID NO;

    2;

    (b) cleaving the product of (a) with a modification-dependent endonuclease that recognizes at least one of the modified nucleotides; and

    (c) differentiating the 5-mC from the 5-hmC by an altered cleavage pattern.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×