Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes

• US 9,464,319 B2
• Filed: 05/09/2012
• Issued: 10/11/2016
• Est. Priority Date: 03/24/2009
• Status: Active Grant

• Current Assignee: University of Chicago
• Original Assignee: California Institute of Technology, University of Chicago
• Inventors: Ismagilov, Rustem F., Shen, Feng, Kreutz, Jason E., Du, Wenbin, Sun, Bing
• Primary Examiner(s): HURST, JONATHAN M
• Application Number:
  US13/467,482
• Publication Number:
  US 20120329038A1
• Time in Patent Office: 1,616 Days
• US Class Current: 1/1
• CPC Class Codes: B01F 25/14 Mixing drops, droplets or bodies of liquid which flow together or contact each other micromixers using where the materials to be mixed are in the form of droplets B01F33/302
• CPC Class Codes: B01F 33/304 the mixing being performed in a mixing chamber where the products are brought into contact
• CPC Class Codes: B01F 35/71411 rotating or oscillating about an axis
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2300/045 whereby the whole cover is slidable
• CPC Class Codes: B01L 2300/049 Valves integrated in closure
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0644 rotary valves
• CPC Class Codes: B01L 2400/065 sliding valves
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: C12Q 1/6851 Quantitative amplification
• CPC Class Codes: C12Q 2531/119 Strand displacement amplification [SDA]
• CPC Class Codes: C12Q 2537/149 Sequential reactions
• CPC Class Codes: C12Q 2563/159 : Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
• CPC Class Codes: C12Q 2565/629 : being a microfluidic device

Abstract

Provided are devices comprising multivolume analysis regions, the devices being capable of supporting amplification, detection, and other processes. Also provided are related methods of detecting or estimating the presence nucleic acids, viral levels, and other biological markers of interest.

Claims

1. A device, comprising:

a first component and a second component;

a first population of areas disposed in said first component and said second component;

a second population of areas disposed in said first component and said second component; and

an inlet,

wherein the first component and the second component are engageable with one another such that a plurality of the first population of areas disposed in said first component and a plurality of the first population of areas disposed in said second component are in fluidic communication with the inlet via a continuous fluidic path within said first and second components, wherein relative motion between the first component and the second component isolates the plurality of the first population of areas disposed in said first component from the plurality of the first population of areas disposed in said second component and exposes at least some of the first population of areas to at least some of the second population of areas so as to form a plurality of analysis regions, at least some of the analysis regions differing in volume from others of the analysis regions, and wherein the first component and the second component are engaged with each other before and after the relative motion.

2. The device of claim 1, wherein the first and second components are engaged so as to permit rotational motion of one component relative to the other component.

3. The device of claim 1, wherein the first and second components are engaged so as to permit linear movement of one component relative to the other component.

4. The device of claim 1, wherein at least one of the first or second components has a thickness in the range of from about 10 micrometers to about 5000 micrometers.

5. The device of claim 1, wherein at least one first area defines a well having a volume in the range of from about 0.1 picoliter to about 10 microliters.

6. The device of claim 1, wherein at least one second area defines a well having a volume in the range of from about 0.1 picoliter to about 10 microliters.

7. The device of claim 1, wherein at least one analysis region defines a volume in the range of from about 0.1 picoliter to about 20 microliters.

8. The device of claim 1, wherein the ratio of the volumes defined by two analysis regions is in the range of from about 1:

1 to about 1;

1,000,000.

9. The device of claim 1, further comprising an imager configured to capture at least one image of an analysis region.

10. The device of claim 1, wherein the device is configured to display an image of an analysis region for capture of at least one image by an imager.

11. The device of claim 10, wherein the imager is a mobile phone camera.

12. The device of claim 9, further comprising a processor configured to estimate a concentration of an analyte residing in one or more analysis regions.

13. The device of claim 1, wherein at least one first area defines a volume that differs from the volume defined by at least one second area.

14. The device of claim 1, wherein the device is adapted so as to be capable of placing at least about 10 first areas into pairwise exposure with at least 10 second areas.

15. The device of claim 14, wherein the device is adapted so as to be capable of placing at least about 100 first areas into pairwise exposure with at least 100 second areas.

16. The device of claim 15, wherein the device is adapted so as to be capable of placing at least about 200 first areas into pairwise exposure with at least 200 second areas.

17. The device of claim 1, further comprising a quantity of a reagent disposed within the device.

18. The device of claim 17, wherein the reagent is useful in an amplification reaction.

19. A device, comprising:

a first component and a second component

a first population of wells disposed in a surface of the first component and in a surface of the second component, the first population of wells arranged in each surface in a radial pattern;

a second population of wells disposed in said surface of the first component and in the surface of the second component, the second population of wells arranged in each surface in a radial pattern; and

an inlet,

the first component and the second components being engageable with one another such that a plurality of the first population of wells disposed in said first component and a plurality of the first population of wells disposed in said second component are in fluidic communication with the inlet via a continuous fluidic path within said first and second components, wherein relative rotational motion between the first component and the second component isolates the plurality of the first population of wells disposed in said first component from the plurality of the first population of wells disposed in said second component and exposes at least some of the first population of wells to at least some of the second population of wells so as to form a plurality of analysis regions, an analysis region comprising a first well and a second well being in pairwise exposure with one another, and wherein the first component and the second component are engaged with each other before and after the relative rotational motion.

20. The device of claim 19, wherein at least two analysis regions have volumes that differ from one another.

21. The device of claim 19, wherein the first component comprises a channel having the inlet, the channel configured so as to place at least some of the first wells into fluidic communication with the environment exterior to the channel.

22. The device of claim 21, wherein the inlet resides in a surface of the first component other than the surface of the first component in which the first wells are formed.

23. The device of claim 19, wherein the device comprises from about 10 to about 10,000 first wells.

24. The device of claim 19, wherein the device comprises from about 10 to about 10,000 second wells.