Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
First Claim
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1. A device, comprising:
- a first component and a second component;
a first population of areas disposed in said first component and said second component;
a second population of areas disposed in said first component and said second component; and
an inlet,wherein the first component and the second component are engageable with one another such that a plurality of the first population of areas disposed in said first component and a plurality of the first population of areas disposed in said second component are in fluidic communication with the inlet via a continuous fluidic path within said first and second components, wherein relative motion between the first component and the second component isolates the plurality of the first population of areas disposed in said first component from the plurality of the first population of areas disposed in said second component and exposes at least some of the first population of areas to at least some of the second population of areas so as to form a plurality of analysis regions, at least some of the analysis regions differing in volume from others of the analysis regions, and wherein the first component and the second component are engaged with each other before and after the relative motion.
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Abstract
Provided are devices comprising multivolume analysis regions, the devices being capable of supporting amplification, detection, and other processes. Also provided are related methods of detecting or estimating the presence nucleic acids, viral levels, and other biological markers of interest.
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Citations
24 Claims
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1. A device, comprising:
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a first component and a second component; a first population of areas disposed in said first component and said second component; a second population of areas disposed in said first component and said second component; and an inlet, wherein the first component and the second component are engageable with one another such that a plurality of the first population of areas disposed in said first component and a plurality of the first population of areas disposed in said second component are in fluidic communication with the inlet via a continuous fluidic path within said first and second components, wherein relative motion between the first component and the second component isolates the plurality of the first population of areas disposed in said first component from the plurality of the first population of areas disposed in said second component and exposes at least some of the first population of areas to at least some of the second population of areas so as to form a plurality of analysis regions, at least some of the analysis regions differing in volume from others of the analysis regions, and wherein the first component and the second component are engaged with each other before and after the relative motion. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A device, comprising:
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a first component and a second component a first population of wells disposed in a surface of the first component and in a surface of the second component, the first population of wells arranged in each surface in a radial pattern; a second population of wells disposed in said surface of the first component and in the surface of the second component, the second population of wells arranged in each surface in a radial pattern; and an inlet, the first component and the second components being engageable with one another such that a plurality of the first population of wells disposed in said first component and a plurality of the first population of wells disposed in said second component are in fluidic communication with the inlet via a continuous fluidic path within said first and second components, wherein relative rotational motion between the first component and the second component isolates the plurality of the first population of wells disposed in said first component from the plurality of the first population of wells disposed in said second component and exposes at least some of the first population of wells to at least some of the second population of wells so as to form a plurality of analysis regions, an analysis region comprising a first well and a second well being in pairwise exposure with one another, and wherein the first component and the second component are engaged with each other before and after the relative rotational motion. - View Dependent Claims (20, 21, 22, 23, 24)
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Specification