Analyte sensors and methods of use

• US 9,465,005 B2
• Filed: 07/22/2015
• Issued: 10/11/2016
• Est. Priority Date: 12/22/2006
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Wang, Yi
• Primary Examiner(s): LARKIN, DANIEL SEAN
• Application Number:
  US14/806,423
• Publication Number:
  US 20150323488A1
• Time in Patent Office: 447 Days
• Field of Search: 73/614.1, 738/647.3, 204/403.1, 204/403.11, 204/403.12, 422/68.1
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/416 Systems G01N27/27 takes precedence
• CPC Class Codes: Y10T 156/1082 Partial cutting bonded sandwich [e.g., grooving or incising]

Abstract

Analyte sensors for determining the concentration of an analyte in a sample. The sensors have a sample chamber having an inlet with a projection extending from an edge of the sensor for facilitating flow of sample into the sample chamber.

Claims

1. A method of determining concentration of an analyte, comprising:

depositing a sample of biological fluid on a biosensor positioned in an analyte meter, the biosensor comprising;

a first substrate, a second substrate, and a spacer layer therebetween forming the biosensor, wherein the biosensor comprises a proximal end and a distal end;

a sample chamber defined between the first substrate and the second substrate bounded by the spacer layer at the proximal end of the biosensor, the sample chamber having a first inlet and a second inlet; and

a first protrusion extending from the first substrate at the first inlet and a second protrusion extending from the first substrate at the second inlet;

electrolyzing the sample; and

determining the concentration of analyte in the biological fluid sample.

2. The method of claim 1, wherein the proximal end of the biosensor has a width and the distal end has a width, and the width of the proximal end is less than the width of the distal end.

3. The method of claim 1, further comprising a third protrusion extending from the second substrate at the first inlet.

4. The method of claim 3, further comprising a fourth protrusion extending from the second substrate at the second inlet.

5. The method of claim 1, wherein a width of each of the protrusion is less than the width of the inlets.

6. The method of claim 5, wherein the first or second protrusion has a height of at least about 0.2 mm.

7. The method of claim 5, wherein the first or second protrusion is a triangle extending from the substrates.

8. The method of claim 1, wherein the sample is deposited at one of the first inlet or the second inlet.

9. The method of claim 1, wherein prior to depositing a surface from a skin of a subject is lanced to present a drop of biological fluid at the surface thereof.

10. The method of claim 9, wherein depositing comprises bringing one of the first protrusion of the second protrusion in contact with the drop of biological fluid.

11. The method of claim 9, wherein the biological fluid is blood.

12. The method of claim 1, wherein determining the concentration of analyte in the sample comprises employing one of coulometry, amperometry and potentiometry.

13. The method of claim 1, wherein determining the concentration of analyte in the sample comprises employing coulometry.

14. The method of claim 1, wherein depositing comprises transferring no more than 1 μ

L of sample into the sample chamber of the biosensor.

15. The method of claim 1, wherein depositing comprises transferring no more than 0.5 μ

L of sample into the sample chamber of the biosensor.

16. The method of claim 1, wherein a width of each of the first and second protrusions is less than a width of the first or second inlet.

17. The method of claim 1, wherein a width of each of the first and second protrusions is no more than about 80% of a width of the first or second inlets.

18. The method of claim 1, wherein the sample chamber has a volume of one microliter or less.