Biomimetic coating of magnesium alloy for enhanced corrosion resistance and calcium phosphate deposition

• US 9,469,677 B2
• Filed: 11/13/2014
• Issued: 10/18/2016
• Est. Priority Date: 11/13/2013
• Status: Active Grant

• Current Assignee: University of Pittsburgh of The Commonwealth System of Higher Education
• Original Assignee: University of Pittsburgh of The Commonwealth System of Higher Education
• Inventors: Beniash, Elia, Sfeir, Charles S.
• Primary Examiner(s): KEMMERER, ELIZABETH
• Application Number:
  US14/540,575
• Publication Number:
  US 20150132356A1
• Time in Patent Office: 705 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A61L 2300/25 Peptides having up to 20 amino acids in a defined sequence
• CPC Class Codes: A61L 2300/252 Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2300/606 Coatings
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 31/022 Metals or alloys
• CPC Class Codes: A61L 31/047 Other specific proteins or polypeptides not covered by A61L31/044 - A61L31/046
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: A61L 31/16 Biologically active materials, e.g. therapeutic substances A61L31/047 takes precedence
• CPC Class Codes: C07K 14/47 from mammals

Abstract

The invention relates to biomimetic peptide-containing compositions for use, in particular, for application and or deposition on a magnesium alloy, e.g., substrate, to at least partially coat a surface of the magnesium alloy. The invention also relates to the coated magnesium alloy which is particularly useful for tissue and bone repair and regeneration applications, such as, for constructing medical implant devices.

Claims

1. A composition for coating magnesium alloy, comprising:

a peptide, which comprises;

RRRDEDE(SSD)_nDEG, wherein n is an integer selected from 3 and 5, indicated by SEQ NO. 2.

2. The composition of claim 1, wherein the peptide comprises:

RRRDEDESSDSSDSSDDEG, indicated by SEQ NO. 2.

3. A coated magnesium alloy substrate, comprising:

a coating composition, which comprises;

a peptide comprising;

RRRDEDE(SSD)_nDEG, wherein n is an integer selected from 3 and 5, indicated by SEQ NO. 2.

4. The coated magnesium alloy substrate of claim 3, wherein the peptide comprises:

RRRDEDESSDSSDSSDDEG, indicated by SEQ NO. 2.

5. A method of preparing a biomimetic magnesium alloy medical device, the method comprising:

making a coating composition comprising a peptide, which comprises;

RRRDEDE(SSD)_nDEG, wherein n is an integer selected from 3 and 5, indicated by SEQ NO. 2;

depositing the coating composition on a least a portion of an outer surface of an uncoated magnesium alloy medical device to form a coating thereon; and

producing a coated magnesium alloy medical device.

6. The method of claim 5, further comprising implanting the coated magnesium alloy medical device into the body of a patient.

7. The method of claim 5, wherein the forming and the depositing of the coating composition, comprises:

obtaining a peptide in the form of a powder, which comprises;

RRRDEDE(SSD)_nDEG, wherein n is an integer selected from 3 and 5, indicated by SEQ NO. 2;

dissolving the peptide in an organic solvent to form a peptide solution;

combining the peptide solution with calcium phosphate solution to form a peptide/calcium phosphate bath solution;

immersing the uncoated magnesium alloy medical device into the peptide/calcium phosphate bath solution to form the coating on the surface of the magnesium medical device.

8. The method of claim 7, wherein the organic solvent is tetrahydrofuran.