Modified polymerases for replication of threose nucleic acids
First Claim
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1. A purified threose nucleic acid (TNA) polymerase comprising an amino acid sequence at least 95% identical to SEQ ID NO:
- 1, wherein, the residue corresponding to 141 of SEQ ID NO;
1 is an alanine, residue 143 is an alanine, residue 485 is an arginine, and residue 664 is an isoleucine; and
wherein the encoded DNA polymerase synthesizes a TNA in the presence of a DNA template and threose nucleotides.
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Abstract
Methods and compositions for replication of threose nucleic acids (TNAs) are described. The described methods include a method for transcribing a DNA template into a TNA, and a method for reverse transcribing a threose nucleic acid into a cDNA.
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Citations
6 Claims
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1. A purified threose nucleic acid (TNA) polymerase comprising an amino acid sequence at least 95% identical to SEQ ID NO:
- 1, wherein, the residue corresponding to 141 of SEQ ID NO;
1 is an alanine, residue 143 is an alanine, residue 485 is an arginine, and residue 664 is an isoleucine; and
wherein the encoded DNA polymerase synthesizes a TNA in the presence of a DNA template and threose nucleotides. - View Dependent Claims (2, 3, 4)
- 1, wherein, the residue corresponding to 141 of SEQ ID NO;
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5. A method for synthesizing a TNA, comprising contacting a DNA template with:
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(i) a purified TNA polymerase comprising an amino acid sequence at least 95% identical to SEQ ID NO;
1, wherein, the residue corresponding to 141 of SEQ ID NO;
1 is an alanine, residue 143 is an alanine, residue 485 is an arginine, and residue 664 is an isoleucine; and
wherein the encoded DNA polymerase synthesizes a TNA in the presence of a DNA template and threose nucleotides; and(ii) threose nucleotides under conditions that permit TNA polymerization, whereby a TNA is synthesized by the TNA polymerase. - View Dependent Claims (6)
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Specification