Method of tissue repair using a piezoelectric scaffold

• US 9,476,026 B2
• Filed: 10/12/2012
• Issued: 10/25/2016
• Est. Priority Date: 03/12/2009
• Status: Active Grant

• Current Assignee: New Jersey Institute of Technology
• Original Assignee: New Jersey Institute of Technology
• Inventors: Lee, Yee-Shuan, Arinzeh, Treena, Collins, George
• Primary Examiner(s): Lankford, Blaine
• Application Number:
  US13/651,296
• Publication Number:
  US 20130052254A1
• Time in Patent Office: 1,474 Days
• US Class Current: 1/1
• CPC Class Codes: A61L 2300/414 Growth factors
• CPC Class Codes: A61L 2400/12 Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 27/16 obtained by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61P 19/04 for non-specific disorders of the connective tissue
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: C08L 27/12 containing fluorine atoms
• CPC Class Codes: C08L 27/16 Homopolymers or copolymers or vinylidene fluoride
• CPC Class Codes: C12N 2529/00 Culture process characterised by the use of electromagnetic stimulation
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0618 Cells of the nervous system
• CPC Class Codes: C12N 5/0668 Mesenchymal stem cells from other natural sources

Abstract

Provided is an electroactive structure and method for growing isolated differentiable cells comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material, wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure.

Claims

1. A method for repairing an injury to bone or cartilage tissue in a subject comprising the steps of

preparing an electroactive scaffold comprising

a three dimensional matrix of electrospun poly(vinylidene fluoride trifluoroethylene) (PVDF-TrFE) copolymer fibers formed by electrospinning the copolymer at an electric potential of at least between 15 to 30 kV, and

a growth factor capable of promoting the differentiation of the mesenchymal stem cell into a osteogenic or chondrogenic phenotype,

wherein the fiber matrix forms a scaffold for supporting cell growth and differentiation;

and wherein the scaffold conditions are sufficient to induce differentiation of a mesenchymal stem cell into either an osteogenic or chondrogenic phenotype; and

implanting the scaffold at the site of injury in the subject, wherein the new bone or cartilage progenitor is formed on the scaffold thereby repairing the injury.

2. The method of claim 1, wherein the method further comprises seeding the scaffold with an isolated differentiable bone or cartilage progenitor cell and allowing the bone or cartilage progenitor cell to grow thereon prior to implanting the scaffold.

3. The method of claim 1, wherein the growth factor is associated with the fiber matrix through at least one of a covalent interaction, a non-covalent interaction or a combination of both.

4. The method of claim 1, wherein the fiber matrix is a non-woven mesh of nanofibers, microfibers or a combination of both.

5. The method of claim 1, wherein the osteogenic or chondrogenic phenotype is demonstrated by at least one of increased collagen expression, growth or a combination thereof.

6. The method of claim 1, wherein the electric potential is 25 kV.

7. The method of claim 1, wherein the growth factor is TGFβ

3.

8. The method of claim 1, wherein the poly(vinylidene fluoride trifluoroethylene) (PVDF-TrFE) copolymer fibers are annealed.