Particle analyzing systems and methods using acoustic radiation pressure

• US 9,476,855 B2
• Filed: 08/21/2014
• Issued: 10/25/2016
• Est. Priority Date: 04/02/2007
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Ward, Michael, Kaduchak, Gregory
• Primary Examiner(s): Xu, Robert
• Application Number:
  US14/464,763
• Publication Number:
  US 20150053561A1
• Time in Patent Office: 796 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A61M 1/3678 Separation of cells using wave pressure; Manipulation of individual corpuscles
• CPC Class Codes: G01N 15/12 by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/1409 Handling samples, e.g. injecting samples
• CPC Class Codes: G01N 15/147 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/1415 Control of particle position
• CPC Class Codes: G01N 2015/142 Acoustic or ultrasonic focussing
• CPC Class Codes: G01N 21/1702 with opto-acoustic detection, e.g. for gases or analysing solids
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 2291/02416 Solids in liquids
• CPC Class Codes: G01N 2291/02809 Concentration of a compound, e.g. measured by a surface mass change
• CPC Class Codes: G01N 2291/02836 Flow rate, liquid level
• CPC Class Codes: G01N 27/447 using electrophoresis
• CPC Class Codes: G01N 29/222 Constructional or flow details for analysing fluids optoacoustic fluid cells G01N29/2425
• CPC Class Codes: G01N 33/56966 Animal cells
• CPC Class Codes: Y10T 436/101666 Particle count or volume standard or control [e.g., platelet count standards, etc.]
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/25625 : Dilution
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

The present invention comprises methods and systems that use acoustic radiation pressure.

Claims

1. A method of analyzing particles with a particle analyzer comprising:

acoustically and hydrodynamically focusing particles to the approximate center of a channel having an electrolytic fluid therein;

flowing the particles through a pore of the channel separating two electrodes between through which an electric current flows; and

detecting the signal wherein the particles displace their own volume of electrolyte momentarily increasing the impedance of the pore to produce the signal.

2. The method of claim 1 wherein the electrolytic fluid is of an engineered conductivity.

3. A fluid for use in the method of claim 2 having an engineered conductivity.

4. The method of claim 1 wherein the particle is a blood cell.

5. The method of claim 1 wherein the particle analyzer is a flow cytometer or cell impedance analyzer.