Compositions and methods for diagnosing and treating kidney disorders in a canine
First Claim
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1. A method of treating, ameliorating, or delaying the progression of a kidney disorder in a canine, comprising the steps of:
- (a) diagnosing the kidney disorder by measuring the level of expression of at least one biomarker in a biological sample from the canine, wherein the at least one biomarker is selected from secreted frizzle-related protein-2 (SFRP2);
matrilin-2 (Matn2);
lumican (LUM);
retinol binding protein 4 (rbp4);
transferrin (TF);
Apo-C-1 (ApoC1); and
inhibin beta A (INHBA), and wherein the test sample is a sample of solid kidney tissue or a bodily fluid;
(b) identifying the canine as having a kidney disorder wherein differences in expression of one or more biomarkers in the sample relative to a control value for expression in a sample from a normal animal indicates the existence of a kidney disorder, and wherein the differences in expression are determined with a custom array consisting of two or more probes specific to the biomarkers selected from matrilin-2 (Matn2);
lumican (LUM);
retinol binding protein 4 (rbp4);
transferrin (TF);
Apo-C-1 (ApoC1); and
inhibin beta A (INHBA); and
(c) treating, ameliorating, or delaying the progression of the kidney disorder in the canine identified in step (b);
wherein the kidney disorder is indicated by a significant difference in expression of one or more biomarkers relative to control expression values wherein a significant difference in the case of increased expression is an increase of at least twofold and in the case of decreased expression is a decrease of at least 50% and wherein one or more biomarkers is selected from;
matrilin-2 (Matn2);
lumican (LUM);
retinol binding protein 4 (rbp4);
transferrin (TF);
Apo-C-1 (ApoC1); and
inhibin beta A (INHBA), andwherein the canine has normal renal function, wherein normal renal function is determined by one or more of;
glomular filtration rate, urine protein level, blood creatinine level, urine creatinine level, creatinine clearance and blood urea nitrogen.
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Abstract
The present invention provides methods for: diagnosing of, devising and monitoring a treatment plan for, and monitoring the status of a kidney disorder characterized by an abnormal loss of renal function, renal failure, reduced glomerular filtration rate or glomerulonephritis, in a canine, wherein the kidney disorder is detectable by utilizing at least one relevant biomarker isolated and measured from a biological test sample taken from such canine. The invention additionally relates to compositions, reagents and kits for carrying out the specified methods.
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Citations
5 Claims
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1. A method of treating, ameliorating, or delaying the progression of a kidney disorder in a canine, comprising the steps of:
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(a) diagnosing the kidney disorder by measuring the level of expression of at least one biomarker in a biological sample from the canine, wherein the at least one biomarker is selected from secreted frizzle-related protein-2 (SFRP2);
matrilin-2 (Matn2);
lumican (LUM);
retinol binding protein 4 (rbp4);
transferrin (TF);
Apo-C-1 (ApoC1); and
inhibin beta A (INHBA), and wherein the test sample is a sample of solid kidney tissue or a bodily fluid;(b) identifying the canine as having a kidney disorder wherein differences in expression of one or more biomarkers in the sample relative to a control value for expression in a sample from a normal animal indicates the existence of a kidney disorder, and wherein the differences in expression are determined with a custom array consisting of two or more probes specific to the biomarkers selected from matrilin-2 (Matn2);
lumican (LUM);
retinol binding protein 4 (rbp4);
transferrin (TF);
Apo-C-1 (ApoC1); and
inhibin beta A (INHBA); and(c) treating, ameliorating, or delaying the progression of the kidney disorder in the canine identified in step (b); wherein the kidney disorder is indicated by a significant difference in expression of one or more biomarkers relative to control expression values wherein a significant difference in the case of increased expression is an increase of at least twofold and in the case of decreased expression is a decrease of at least 50% and wherein one or more biomarkers is selected from;
matrilin-2 (Matn2);
lumican (LUM);
retinol binding protein 4 (rbp4);
transferrin (TF);
Apo-C-1 (ApoC1); and
inhibin beta A (INHBA), andwherein the canine has normal renal function, wherein normal renal function is determined by one or more of;
glomular filtration rate, urine protein level, blood creatinine level, urine creatinine level, creatinine clearance and blood urea nitrogen. - View Dependent Claims (2, 3, 4, 5)
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Specification