Sensor

• US 9,476,886 B2
• Filed: 01/26/2015
• Issued: 10/25/2016
• Est. Priority Date: 03/15/2012
• Status: Active Grant

• Current Assignee: Baxter Healthcare SA (Baxter International Inc.), Baxter International Inc.
• Original Assignee: Lightship Medical Limited
• Inventors: Paterson, William, Barwell, Nick, Culbert, Bruce, Crane, Barry Colin
• Primary Examiner(s): Wallenhorst, Maureen
• Application Number:
  US14/605,315
• Publication Number:
  US 20150219663A1
• Time in Patent Office: 638 Days
• Field of Search: 436/63, 436/95, 436/127, 436/135, 436/73, 436/80, 436/84, 436/164, 436/165, 436/166, 436/172, 436/174, 436/178, 436/501, 435/14, 435/7.1, 435/288.7, 422/82.05, 422/82.08, 422/82.11, 422/534, 422/535, 422/69
• US Class Current: 1/1
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: A61B 5/14735 comprising an immobilised reagent
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/206664 Ozone or peroxide
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A sensor for detecting and/or quantifying the amount of analyte in a sample, the sensor including:

• • a sensing region; and
  • a barrier layer including a reactive oxygen species (ROS)-quenching, analyte-permeable membrane having an ROS-quenching agent adsorbed thereto;
    wherein the sensor is adapted so that the sample enters the sensing region of the sensor through said barrier layer.

Claims

1. A sensor for detecting and/or quantifying the amount of analyte in a sample, the sensor comprising:

a sensing region; and

a barrier layer comprising a reactive oxygen species (ROS)-quenching, analyte-permeable membrane having an ROS-quenching agent within pores of the membrane, the pores each having a length, wherein the ROS-quenching agent is distributed along the length of the pores; and

wherein the sensor is configured so that the sample enters the sensing region of the sensor through said barrier layer.

2. A sensor according to claim 1, wherein the membrane selectively quenches ROS.

3. A sensor according to claim 2, wherein the membrane substantially does not cause depletion of analyte.

4. A sensor according to claim 1 which is an optical sensor comprising an optical waveguide for directing incident light onto the sensing region.

5. A sensor according to claim 4, which is an optical glucose sensor comprising a sensing region comprising a boronic acid receptor for binding to glucose and a fluorophore associated with said receptor.

6. A sensor according to claim 1, wherein the ROS-quenching agent is a transition metal, a transition metal compound, or an enzyme.

7. A sensor according to claim 6 wherein the ROS-quenching agent is a transition metal of Group 10 or 11 of the Periodic Table or an alloy containing a transition metal of Group 10 or 11 of the Periodic Table.

8. A sensor according to claim 7, wherein the ROS-quenching agent is platinum in the form of nanoparticles.

9. A sensor according to claim 7, wherein the ROS-quenching agent is platinum.

10. The sensor of claim 1, wherein the pores in the membrane have an aspect ratio of at least 100.

11. The sensor of claim 10, wherein the membrane is a hollow fiber membrane.

12. The sensor of claim 10, wherein the membrane has a thickness of between 20 and 50 microns.

13. The sensor of claim 10, wherein the pores in the membrane have a pore diameter of 100 nm or less.

14. The sensor of claim 13, wherein the ROS-quenching agent is in the form of particles having a nanoscale average particle size of between 1 nm and 100 nm.

15. A reactive oxygen species (ROS)-quenching, analyte-permeable membrane having pores, configured for use in a sensor for detecting and/or quantifying the amount of analyte in a sample, the membrane having an ROS-quenching agent within the pores of the membrane, wherein each pore of the membrane has a length and the ROS-quenching agent is distributed along the length of the pores.

16. A membrane according to claim 15 wherein the ROS-quenching agent is a transition metal, a transition metal compound, or an enzyme.

17. A membrane according to claim 15 which selectively quenches ROS.

18. A method of detecting and/or quantifying the amount of analyte in a sample, comprising inserting into the sample a sensor, providing incident light to a sensing region of the sensor and detecting an emission pattern of a fluorophore;

wherein the sensor is configured for detecting and/or quantifying the amount of analyte in a sample and comprises;

a sensing region; and

a barrier layer comprising a reactive oxygen species (ROS)-quenching, analyte-permeable membrane having an ROS-quenching agent within pores of the membrane, each pore of the membrane has a length and the ROS-quenching agent is distributed along the length of the pores;

wherein the sensor is configured so that the sample enters the sensing region of the sensor through said barrier layer.