Methods of using adipose-derived regenerative cells in the treatment of peripheral vascular disease and related disorders
First Claim
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1. A method for treating peripheral vascular disease (PVD) in a subject, comprising:
- identifying a subject with PVD;
obtaining a unit of adipose tissue from said subject;
processing said adipose tissue by separating adipose-derived cells comprising stem and progenitor cells from mature adipocytes and connective tissue, and washing, separating, and concentrating said adipose-derived cells from adipose tissue to obtain a concentrated population of cells comprising adipose-derived stem and endothelial progenitor cells, wherein at least 0.1% of the adipose-derived cells are adipose-derived stem cells; and
providing to the subject an amount of said concentrated population of adipose-derived cells that is sufficient to restore blood flow to an ischemic region, wherein said concentrated population of adipose-derived cells is not grown in culture.
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Abstract
Cells present in adipose tissue are used to treat patients, including patients with PVD and related diseases or disorders. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Accordingly, in a preferred method, cells present in adipose tissue are placed directly into a recipient along with such additives necessary to promote, engender or support a therapeutic benefit.
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Citations
21 Claims
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1. A method for treating peripheral vascular disease (PVD) in a subject, comprising:
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identifying a subject with PVD; obtaining a unit of adipose tissue from said subject; processing said adipose tissue by separating adipose-derived cells comprising stem and progenitor cells from mature adipocytes and connective tissue, and washing, separating, and concentrating said adipose-derived cells from adipose tissue to obtain a concentrated population of cells comprising adipose-derived stem and endothelial progenitor cells, wherein at least 0.1% of the adipose-derived cells are adipose-derived stem cells; and providing to the subject an amount of said concentrated population of adipose-derived cells that is sufficient to restore blood flow to an ischemic region, wherein said concentrated population of adipose-derived cells is not grown in culture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification