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Methods of genome installation in a recipient host cell

  • US 9,481,880 B2
  • Filed: 05/01/2008
  • Issued: 11/01/2016
  • Est. Priority Date: 05/01/2007
  • Status: Active Grant
First Claim
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1. A method for transplantation of a Mycoplasma mycoides Large Colony donor cell genome in a recipient Mycoplasma capricolum, said method comprising:

  • (a) isolating a closed circular double stranded donor genome from a Mycoplasma mycoides Large Colony donor cell and suspending the isolated donor genome in agarose;

    (b) deproteinizing the donor genome and liberating the donor genome from the agarose;

    (c) preparing a Mycoplasma capricolum as a recipient cell for introduction of the donor genome by removing or inactivating the recipient cell genome, and simultaneously introducing the isolated donor genome from step (b) into the recipient cell in presence of polyethylene glycol, wherein the donor genome phenotypically transforms the recipient cell to that of the of Mycoplasma mycoides Large Colony.

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