Arrays and methods of use
First Claim
Patent Images
1. A method of producing a molecular array comprising:
- providing a solid support comprising a plurality of physically discrete elements, wherein one element of said plurality of physically discrete elements is separated from another element of said plurality of physically discrete elements by one or more raised regions or trenches and each of said plurality of physically discrete elements is arranged at predetermined locations on said solid support and is separated by a fixed distance; and
immobilizing a plurality of target oligonucleotide molecules onto said plurality of physically discrete elements, thereby producing immobilized target oligonucleotide molecules and a molecular array comprising said plurality of physically discrete elements having said immobilized target oligonucleotide molecules, wherein each of said plurality of physically discrete elements of the molecular array contains only one of said immobilized target oligonucleotide molecules.
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Abstract
Methods are provided for producing a molecular array comprising a plurality of molecules immoblized to a solid substrate at a density which allows individual immobilized molecules to be individually resolved, wherein each individual molecule in the array is spatially addressable and the identity of each molecule is known or determined prior to immobilization. The use of spatially addressable low density molecular arrays in single molecule detection and analysis techniques is also provided. Novel assays and methods are also provided.
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Citations
24 Claims
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1. A method of producing a molecular array comprising:
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providing a solid support comprising a plurality of physically discrete elements, wherein one element of said plurality of physically discrete elements is separated from another element of said plurality of physically discrete elements by one or more raised regions or trenches and each of said plurality of physically discrete elements is arranged at predetermined locations on said solid support and is separated by a fixed distance; and immobilizing a plurality of target oligonucleotide molecules onto said plurality of physically discrete elements, thereby producing immobilized target oligonucleotide molecules and a molecular array comprising said plurality of physically discrete elements having said immobilized target oligonucleotide molecules, wherein each of said plurality of physically discrete elements of the molecular array contains only one of said immobilized target oligonucleotide molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method of producing a molecular array comprising:
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providing a solid support comprising a plurality of physically discrete wells, wherein each of said plurality of physically discrete wells is arranged at predetermined locations of said solid support; and immobilizing a plurality of target oligonucleotide molecules into said physically discrete wells, thereby producing immobilized target oligonucleotide molecules and a molecular array comprising said plurality of physically discrete wells having said immobilized target oligonucleotide molecules, wherein each of said plurality of physically discrete wells of the molecular array contains only one of said immobilized target oligonucleotide molecules. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23)
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24. A method of producing a molecular array comprising:
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providing a solid support comprising a plurality of physically discrete wells, wherein each of said plurality of physically discrete wells is arranged at predetermined locations of said solid support; immobilizing a plurality of target oligonucleotide molecules into said physically discrete wells, thereby producing immobilized target oligonucleotide molecules and an array comprising said plurality of physically discrete wells having said immobilized target oligonucleotide molecules, wherein each of said plurality of physically discrete wells of the array contains only one of said immobilized target oligonucleotide molecules; amplifying said only one of said immobilized target oligonucleotide molecules in each of said plurality of physically discrete wells and creating multiple copies of said only one of said immobilized target oligonucleotide molecules in each of said plurality of physically discrete wells, said multiple copies of said only one of said immobilized target oligonucleotide molecules in each of said plurality of physically discrete wells are a single molecule species; and labeling at least a portion of said multiple copies of said only one of said immobilized target oligonucleotide molecules with one or more labels, thereby producing a molecular array comprising labeled said only one of said immobilized target oligonucleotide molecules in each of said plurality of physically discrete wells.
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Specification