Methods of inducing regulated pancreatic hormone production in non-pancreatic islet tissues
First Claim
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1. An in vitro method of increasing PDX-1 induction of a pancreatic islet cell phenotype or function in a population of human non-pancreatic primary cells, said method comprising the steps of:
- (1) contacting a population of human non-pancreatic primary cells with a composition comprising a nucleic acid encoding a PDX-1 polypeptide, wherein said PDX-1 is ectopically expressed within said cell population,(2) concurrently contacting said cell population with a composition comprising an effective amount of nicotinamide and epidermal growth factor; and
(3) measuring the expression levels of PDX-1 in the contacted cells of step (2),wherein said concurrent contacting of said population with a composition comprising an effective amount of nicotinamide and epidermal growth factor increases PDX-1 induction of a pancreatic islet cell phenotype or function.
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Abstract
Disclosed are methods and pharmaceutical compositions for inducing pancreatic hormone production.
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Citations
9 Claims
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1. An in vitro method of increasing PDX-1 induction of a pancreatic islet cell phenotype or function in a population of human non-pancreatic primary cells, said method comprising the steps of:
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(1) contacting a population of human non-pancreatic primary cells with a composition comprising a nucleic acid encoding a PDX-1 polypeptide, wherein said PDX-1 is ectopically expressed within said cell population, (2) concurrently contacting said cell population with a composition comprising an effective amount of nicotinamide and epidermal growth factor; and (3) measuring the expression levels of PDX-1 in the contacted cells of step (2), wherein said concurrent contacting of said population with a composition comprising an effective amount of nicotinamide and epidermal growth factor increases PDX-1 induction of a pancreatic islet cell phenotype or function. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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