Instrument and method for detecting analytes

• US 9,482,613 B2
• Filed: 05/16/2012
• Issued: 11/01/2016
• Est. Priority Date: 05/16/2011
• Status: Active Grant

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Roche Molecular Systems (Roche Holding AG)
• Inventors: Furlan, Alan
• Primary Examiner(s): Turk, Neil N
• Application Number:
  US13/473,010
• Publication Number:
  US 20120295268A1
• Time in Patent Office: 1,630 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: G01N 2021/3174 Filter wheel
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 2021/6471 Special filters, filter wheel
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 2201/0423 with rotating optics
• CPC Class Codes: G01N 2201/0668 Multiple paths; optimisable path length
• CPC Class Codes: G02B 7/006 Filter holders

Abstract

The present disclosure provides instruments and methods for detecting an analyte which are capable of exciting a plurality of luminescence labels and detecting light emitted therefrom. The instrument includes a filter carrier adapted for carrying a plurality of filter portion pairs, each pair related to a luminescence label and comprising a first filter portion for transmitting excitation light, and a second filter portion for transmitting emitted light. The first filter portion of a pair comprises a second filter portion of another pair. Also, the filter portions are arranged such that a pair can be brought into an operative condition whereby a first filter portion is in the excitation beam path and a second filter portion is in the emission beam path. The filter carrier and beam paths may be moved with respect to each other by a moving mechanism so as to bring a pair into operative condition.

Claims

1. An instrument for detecting an emission light emitted from one or more of a plurality of luminescence labels associated with an analyte in a sample, comprising:

a mount configured to support a vessel for housing one or more samples;

a light source capable of generating an excitation light for exciting said plurality of luminescence labels associated with said analyte in one or more samples housed in said vessel;

an excitation arrangement configured to propagate said excitation light along an excitation beam path positioned between said light source and said vessel, said excitation beam path comprising one or more excitation light fibers configured to transmit said excitation light to said vessel;

a detector capable of detecting said emission light emitted from one of said plurality of luminescence labels;

a detection arrangement configured to propagate said emission light along an emission beam path positioned between said vessel and said detector, said emission beam path comprising one or more emission light fibers configured to transmit said emission light to said detector;

a filter wheel rotatably mounted to a wheel hub, thereby defining a spin axis, said filter wheel comprising a plurality of filter portion pairs positioned around said wheel hub, each pair relating to one of said plurality of luminescence labels and comprising a first filter portion adapted to filter wavelengths of said excitation light and transmit said excitation light for exciting the related luminescence label, and a second filter portion adapted to filter wavelengths of said emission light and transmit said emission light emitted by said related luminescence label, said first filter portion of one of said plurality of pairs comprising said second filter portion of another of said plurality of pairs, said plurality of filter portion pairs being positioned around said wheel hub such that, upon rotation of the filter wheel about the spin axis, one of said plurality of pairs can be brought into an operative orientation relative to said excitation and emission beam paths such that said first filter portion is positioned in said excitation beam path and said second filter portion is positioned in said emission beam path.

2. The instrument according to claim 1, further comprising a carrier drive operatively connected to the filter wheel and wheel hub and adapted to rotate said filter wheel about said spin axis relative to said excitation and emission beam paths.

3. The instrument according to claim 2, wherein said filter portions are arranged in a manner that one of said plurality of pairs can be moved in said operative orientation by moving said filter wheel by equal rotation steps with respect to said excitation beam path and said emission beam path being stationary.

4. The instrument according to claim 1, wherein said filter wheel includes at least one opaque region adapted to inhibit the transmission of said emission light emitted by one of said plurality of luminescence labels, said opaque region arranged to be positioned in said emission beam path.

5. The instrument according to claim 1, comprising a one-piece filter for providing said first and second filter portions.

6. The instrument according to claim 5, wherein said one-piece filter has a discrete transmission spectrum.

8. The instrument of claim 1 wherein the excitation arrangement comprises one or more additional elements selected from the group consisting of lenses, planar mirrors, bent mirrors, transmission gratings, reflective gratings, prisms, and combinations thereof.

9. The instrument of claim 1 wherein the detection arrangement comprises one or more additional elements selected from the group consisting of lenses, planar mirrors, bent mirrors, transmission gratings, reflective gratings, prisms, and combinations thereof.

7. A system for detecting an emission light emitted from one or more of a plurality of luminescence labels associated with an analyte in a sample, comprising:

a mount configured to support a vessel for housing one or more samples and comprising a temperature-controlled block for heating said vessel comprising said one or more samples;

a light source capable of generating an excitation light for exciting at least one of said plurality of luminescence labels associated with said analyte in said one or more samples;

an excitation arrangement configured to propagate said excitation light along an excitation beam path positioned between said light source and an analyte region, said excitation beam path comprising one or more excitation light fibers configured to transmit said excitation light to said vessel;

a detector capable of detecting an emission light emitted from one of said plurality of luminescence labels;

a detection arrangement configured to propagate said emission light along an emission beam path positioned between said vessel and said detector, said emission beam path comprising one or more emission light fibers configured to transmit said emission light to said detector;

a filter wheel rotatably mounted to a wheel hub, thereby defining a spin axis, said filter wheel comprising two or more pairs of filter portions positioned around said wheel hub, each pair of filter portions being related to one luminescence label and comprising a first filter portion adapted to filter wavelengths of said excitation light and transmit said excitation light for exciting said related luminescence label, and a second filter portion adapted to filter wavelengths of said emission light and transmit said emission light emitted by said related luminescence label, wherein said first filter portion of one pair is said second filter portion of another pair, said filter wheel being movable such that, upon rotation of the filter wheel about the spin axis, one of said pairs are brought into an operative orientation relative to said excitation and emission beam paths such that said first filter portion is in said excitation beam path and said second filter portion is in said emission beam path;

a carrier drive operatively connected to the filter wheel and wheel hub and adapted to rotate said filter wheel about said spin axis relative to said excitation beam path and said emission beam path;

and wherein a controller is configured to control activity of said carrier drive in a manner to move one of said pairs of said first and second filter portions in to said operative orientation.

10. The system of claim 7 wherein the excitation arrangement comprises one or more additional elements selected from the group consisting of lenses, planar mirrors, bent mirrors, transmission gratings, reflective gratings, prisms, and combinations thereof.

11. The system of claim 7 wherein the detection arrangement comprises one or more additional elements selected from the group consisting of lenses, planar mirrors, bent mirrors, transmission gratings, reflective gratings, prisms, and combinations thereof.