×

Ultra-sensitive detection of molecules or particles using beads or other capture objects

  • US 9,482,662 B2
  • Filed: 06/22/2012
  • Issued: 11/01/2016
  • Est. Priority Date: 03/01/2010
  • Status: Active Grant
First Claim
Patent Images

1. A method for determining a measure of the concentration of a plurality of types of analyte molecules or particles, said plurality including at least a first type of analyte molecules or particles and a second type of analyte molecules or particles in a fluid sample, comprising:

  • providing a substrate comprising a plurality of locations, at least a portion of which locations contain a first type of capture object and at least a portion of which locations contain a second type of capture object, wherein with respect to the total number of capture objects present on the substrate, the ratio of capture objects comprising at least one analyte molecule or particle to capture objects comprising no analyte molecules or particles is between about 8;

    1 and about 1;

    10,000,000;

    addressing at least a portion of the plurality of locations, wherein during the addressing step at least two of the plurality of locations is addressed at least partially concurrently;

    detecting at each addressed location the presence or absence of a first type of capture object or the presence or absence of a second type of capture object and whether, if present, the first type of capture object and the second type of capture object is associated with any of the first type of analyte molecules or particles or any of the second type of analyte molecules or particles, respectively;

    determining a measure of the concentration of the first type of analyte molecules or particles in the fluid sample at least in part by determining the fraction of the first type of capture objects subjected to the addressing step determined to be associated with at least one analyte molecule or particle of the first type; and

    determining a measure of the concentration of the second type of analyte molecules or particles in the fluid sample at least in part by determining the fraction of the second type of capture objects subjected to the addressing step determined to be associated with at least one analyte molecule or particle of the second type.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×