Compositions and methods for in vivo evaluation of bioluminescence
First Claim
Patent Images
1. A live non-human mammalian animal comprising:
- (i) an exogenous transgene encoding a bioluminescent protein; and
(ii) a composition comprising a bioluminescent substrate and a fluorescent tracking dye, wherein the bioluminescent substrate and the fluorescent tracking dye emit light of different wavelengths and further wherein the bioluminescent substrate is transformed to an excited state in the presence of an oxygenase, oxygen, and ATP, and wherein the fluorescent tracking dye is not a protein.
0 Assignments
0 Petitions
Accused Products
Abstract
Compositions and methods are described for normalizing a bioluminescent signal in a live animal.
-
Citations
11 Claims
-
1. A live non-human mammalian animal comprising:
- (i) an exogenous transgene encoding a bioluminescent protein; and
(ii) a composition comprising a bioluminescent substrate and a fluorescent tracking dye, wherein the bioluminescent substrate and the fluorescent tracking dye emit light of different wavelengths and further wherein the bioluminescent substrate is transformed to an excited state in the presence of an oxygenase, oxygen, and ATP, and wherein the fluorescent tracking dye is not a protein. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
- (i) an exogenous transgene encoding a bioluminescent protein; and
Specification