Compositions and methods for in vivo evaluation of bioluminescence

• US 9,486,542 B2
• Filed: 01/27/2015
• Issued: 11/08/2016
• Est. Priority Date: 09/18/2009
• Status: Active Grant

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Inventors: Oldfield, Stephen, Ansaldi, Daniel, Singh, Rajendra, Zhang, Ning
• Primary Examiner(s): Noble, Marcia S
• Application Number:
  US14/606,817
• Publication Number:
  US 20150143554A1
• Time in Patent Office: 651 Days
• US Class Current: 1/1
• CPC Class Codes: A01K 2267/03 Animal model, e.g. for test or diseases
• CPC Class Codes: A01K 67/0275 Genetically modified vertebrates, e.g. transgenic
• CPC Class Codes: A61K 49/0013 Luminescence
• CPC Class Codes: A61K 49/0017 Fluorescence in vivo
• CPC Class Codes: A61K 49/0021 the fluorescent group being a small organic molecule
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/763 Bioluminescence
• CPC Class Codes: G01N 33/582 with fluorescent label

Abstract

Compositions and methods are described for normalizing a bioluminescent signal in a live animal.

Claims

1. A live non-human mammalian animal comprising:

(i) an exogenous transgene encoding a bioluminescent protein; and

(ii) a composition comprising a bioluminescent substrate and a fluorescent tracking dye, wherein the bioluminescent substrate and the fluorescent tracking dye emit light of different wavelengths and further wherein the bioluminescent substrate is transformed to an excited state in the presence of an oxygenase, oxygen, and ATP, and wherein the fluorescent tracking dye is not a protein.

2. The live animal of claim 1, wherein the bioluminescent substrate comprises luciferin.

3. The live animal of claim 1, wherein the composition further comprises a buffer.

4. The live animal of claim 1, wherein the tracking dye emits green light.

5. The live animal of claim 1, wherein the tracking dye emits red light.

6. The live animal of claim 1, wherein the tracking dye emits purple light.

7. The live animal of claim 1, wherein the tracking dye emits violet light.

8. The live animal of claim 1, wherein the tracking dye emits blue light.

9. The live animal of claim 1, wherein the tracking dye emits yellow light.

10. The live animal of claim 1, wherein the tracking dye emits orange light.

11. The live animal of claim 1, wherein the tracking dye emits near infra-red (IR) light.