Passive electric field focus system for in vivo and in vitro applications

• US 9,486,626 B2
• Filed: 04/07/2015
• Issued: 11/08/2016
• Est. Priority Date: 04/07/2006
• Status: Active Grant

• Current Assignee: University of South Florida (State University System of Florida)
• Original Assignee: University of South Florida (State University System of Florida)
• Inventors: Rey, Jose I., Gilbert, Richard, Jaroszeski, Mark J., Heller, Richard
• Primary Examiner(s): Flory, Christopher A
• Application Number:
  US14/680,739
• Publication Number:
  US 20150209582A1
• Time in Patent Office: 581 Days
• Field of Search: 607/2, 607/116, 604/20, 435/173.6, 435/285.2
• US Class Current: 1/1
• CPC Class Codes: A61N 1/325 for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body electrodes for external use A61N1/0428
• CPC Class Codes: A61N 1/327 for enhancing the absorption properties of tissue, e.g. by electroporation
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Abstract

The present invention provides a system and method of directing, focusing, or concentrating electrical charges within a defined electric field so that these charges can be used to exert forces on cells and tissues in vivo and/or cell cultures in vitro. The present invention reduces and/or eliminates the damage at a target site that would normally be caused by an electrode that acts as a current source or sink to accomplish the same task.

Claims

1. A system for focusing an electric field within a defined electric field to facilitate electroporation within a biological entity, the system comprising:

at least two active electrodes adapted to be positioned to establish an electric field within the biological entity, the electric field established between the at least two active electrodes and the established electric field having an electric field intensity concentration focus; and

at least one passive electrode adapted to be positioned within the established electric field between the at least two active electrodes, the at least one passive electrode to change the focus of the electric field intensity concentration to facilitate electroporation at a desired location within the biological entity, wherein the passive electrode is fabricated of a material selected from the group consisting of metals, semiconductors, nanoparticles, plastics, polymers, bio-polymers, fluidistic substances, non-conductive materials and high relative permittivity materials, wherein the passive electrode is not a current source or a current sink for the established electric field, wherein the passive electrode is never physically or electrically coupled to a source of electrical energy, wherein the passive electrode is never physically or electrically coupled to another passive electrode or to a conducting element, and wherein the passive electrode exerts electrical forces on the biological entity at the desired focus location.

2. The system of claim 1, further comprising a power source coupled to the at least two active electrodes.

3. The system of claim 1, further comprising software means to control the establishment of the electric field.

4. The system of claim 1, wherein the at least two ac electrodes further comprises a source electrode and a sink electrode.

5. The system of claim 1, wherein the at least two active electrodes and the at least one passive electrode are adapted to be positioned within the biological entity in vivo.

6. The system of claim 1, wherein the at least two active electrodes and the at least one passive electrode are adapted to be positioned within the biological entity in vitro.

7. The system of claim 1, wherein the biological entity is selected from the group consisting of a cell or cells, a cellular tissue and a cell culture.

8. The system of claim 1, wherein the passive electrode is shaped in a star geometry.

9. The system of claim 1, wherein the passive electrode is shaped in a four-corners geometry.

10. The system of claim 1, wherein the passive electrode further comprises a plurality of conductive needles.

11. The system of claim 1, wherein the passive electrode is a metal sphere adapted to be placed beneath a surface layer of the biological entity.

12. The system of claim 11, where the metal sphere further comprises shell particles positioned on the surface of the sphere.

13. The system of claim 1, wherein the passive electrode is a fluid.

14. The system of claim 13, wherein a concentration profile of the fluid passive electrode changes over time.

15. The system of claim 13, wherein a shape of the fluid passive electrode changes over time.

16. The system of claim 1, wherein the passive electrode is removable.

17. The system of claim 1, wherein the passive electrode is a container for an agent.

18. The system of claim 17, wherein the agent is a drug for delivery into the biological entity.

19. The system of claim 1, wherein the passive electrode is a scaffold for biological structures within the biological entity.

20. The system of claim 1, wherein the passive electrode is labeled.

21. The system of claim 1, wherein the passive electrode further comprises a plurality of electrical characteristics and said electrical characteristics vary dependent upon varying environmental conditions.

22. The system of claim 21, wherein one of the plurality of electrical characteristics is conductivity, and said conductivity of the passive electrode changes under varying temperature conditions.