Acousto-electromagnetic investigation of physical properties of an object

• US 9,488,573 B2
• Filed: 09/19/2012
• Issued: 11/08/2016
• Est. Priority Date: 09/23/2011
• Status: Active Grant

• Current Assignee: Oxford University Innovation Limited (University of Oxford)
• Original Assignee: Isis Innovation Limited
• Inventors: Edwards, David John, Vachirimon, Pithawat
• Primary Examiner(s): Saint Surin, J M
• Application Number:
  US14/346,462
• Publication Number:
  US 20140224021A1
• Time in Patent Office: 1,511 Days
• Field of Search: 736/01
• US Class Current: 1/1
• CPC Class Codes: A61B 5/0048 Detecting, measuring or recording by applying mechanical forces or stimuli A61B5/021, A61B5/4884 and A61B9/00 take precedence
• CPC Class Codes: A61B 5/0051 by applying vibrations
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/05 Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; Measuring using microwaves or radio waves impedance plethysmography A61B5/0295; measuring movement of the entire body or parts thereof A61B5/11; detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof A61B5/24
• CPC Class Codes: A61B 5/0507 using microwaves or terahertz waves
• CPC Class Codes: A61B 5/7228 Signal modulation applied to the input signal sent to patient or subject; demodulation to recover the physiological signal modulation H03C
• CPC Class Codes: G01N 21/1702 with opto-acoustic detection, e.g. for gases or analysing solids
• CPC Class Codes: G01N 21/1717 with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance
• CPC Class Codes: G01N 21/3581 using far infrared light; using Terahertz radiation
• CPC Class Codes: G01N 21/47 Scattering, i.e. diffuse reflection G01N21/25, G01N21/41 take precedence G01N21/55 takes precedence
• CPC Class Codes: G01N 2291/017 Doppler techniques
• CPC Class Codes: G01N 29/0654 Imaging
• CPC Class Codes: G01S 13/50 Systems of measurement based on relative movement of target
• CPC Class Codes: G01S 13/862 Combination of radar systems with sonar systems
• CPC Class Codes: G01S 13/89 for mapping or imaging
• CPC Class Codes: G01S 15/899 Combination of imaging systems with ancillary equipment

Abstract

An imaging system for an object such as human or animal tissue applies acoustic vibrations localized in two or three dimensions and simultaneously illuminates the object with an illuminating electromagnetic wave. The acoustic vibration comprises a carrier wave that is amplitude modulated by an AM waveform. The carrier wave is selected to provide the localization of the acoustic vibration, whereas the AM waveform includes a frequency component selected to provide a vibration of the object of greater magnitude than the carrier wave. To detect a Doppler component shifted by the frequency of said frequency component of the AM waveform, a signal processing apparatus comprises a phase locked-loop locked to the EM frequency, producing a frequency-demodulated signal comprising the set of the Doppler components, and a lock-in amplifier configured to extract a signal at a reference frequency equal to the frequency of a frequency component of the AM waveform.

Claims

1. A method of investigating physical properties of an object, comprising:

applying to the object acoustic vibration localised in two or three dimensions in a region in the object, the acoustic vibration comprising a carrier wave that is amplitude modulated by an AM waveform, the carrier wave being selected to provide the localization of the acoustic vibration and the AM waveform including a frequency component selected to provide a vibration of the object of greater magnitude than the carrier wave;

simultaneously illuminating the object with an illuminating electromagnetic wave that has an EM frequency in a range extending up to 30 THz, the vibration direction of the acoustic vibration having a component parallel to the propagation direction of the illuminating electromagnetic wave so that the acoustic vibration of the object in the region generates a scattered electromagnetic wave including a set of Doppler components shifted from the frequency of the illuminating electromagnetic wave by frequencies of the vibration of the object caused by the acoustic vibration and multiples thereof; and

receiving the scattered electromagnetic wave generated in the region,

detecting, from the received, scattered electromagnetic wave, a Doppler component shifted from the frequency of the illuminating electromagnetic wave by the frequency of said frequency component of the AM waveform; and

outputting a signal representing at least one characteristic of the detected Doppler component.

2. The method according to claim 1, wherein said AM waveform has a fundamental frequency component, said frequency component of the AM waveform being the fundamental frequency component.

3. The method according to claim 2, wherein said AM waveform further includes other frequency components.

4. The method according to claim 3, wherein the AM waveform is a square wave.

5. The method according to claim 1, wherein said frequency component of the AM waveform has a period of the same order of magnitude as the acoustic relaxation time of the region of the object.

6. The method according to claim 1, wherein said frequency component of the AM waveform is selected to provide a resonant vibration of the object.

7. The method according to claim 1, wherein the carrier wave has a frequency in the range from 10 kHz to 1 GHz.

8. The method according to claim 1, wherein said frequency component of the AM waveform is in the range from 1 Hz to 100 MHz.

9. The method according to claim 8, wherein said frequency component of the AM waveform is in a range extending up from 10 kHz and/or extending up to 1 kHz.

10. The method according to claim 8, wherein the illuminating electromagnetic wave has a frequency in a range extending up to 2 GHz.

11. The method according to claim 1, wherein the illuminating electromagnetic wave has an EM frequency in a range extending up from a value of twice said frequency component of the AM waveform, preferably up from a value of ten times said frequency component of the AM waveform.

12. The method according to claim 1, wherein said object is a biological object.

13. The method according to claim 12, wherein said biological object is human or animal tissue.

14. The method according to claim 12, wherein the carrier wave has a frequency in a range extending up from 1 MHz and/or extending up to 50 MHz.

15. The method according to claim 1, wherein the step of detecting Doppler components comprises:

supplying the received, scattered electromagnetic wave to a phase-locked loop locked to the EM frequency to produce a frequency-demodulated signal comprising the set of the Doppler components frequency-demodulated from EM frequency; and

supplying the frequency-demodulated signal to a lock-in amplifier configured to extract a signal at a reference frequency equal to the frequency of said frequency component of the AM waveform.

16. The method according to claim 15, wherein the lock-in amplifier being configured to generate the amplitude and/or phase of the extracted signal, being said at least one characteristic of the detected Doppler component.

17. The method according to claim 1, further comprising:

applying, simultaneously with said first mentioned acoustic vibration, a second acoustic vibration localised in two or three dimensions in a further region in the object, the second acoustic vibration comprising a carrier wave that is amplitude modulated by a second AM waveform, the carrier wave being selected to provide the localization of the further acoustic vibration and the second AM waveform including a frequency component of a different frequency from the first mentioned waveform of the first acoustic vibration, selected to provide a vibration of the object of greater magnitude than the carrier wave;

detecting, from the received, scattered electromagnetic wave, a second Doppler component shifted from the frequency of the illuminating electromagnetic wave by the frequency of said frequency component of the second AM waveform; and

outputting a signal representing at least one characteristic of the detected second Doppler component.

18. The method according to claim 1 being a method of imaging an object, wherein:

the step of applying acoustic vibration comprises applying the acoustic vibration localised in a plurality of regions either sequentially or simultaneously but with AM waveforms including frequency components of different frequencies in each region;

the step of receiving the scattered electromagnetic wave comprises receiving the scattered electromagnetic wave generated in each of the plurality of regions;

the step of detecting a Doppler component comprises detecting a Doppler component in respect of each region;

the step of outputting a signal comprises outputting a signal representing at least one characteristic of the detected Doppler component in respect of each region; and

the method further comprises storing data representing the at least one characteristic of the detected Doppler component in respect of each region as image data.

19. A system for investigating physical properties of an object, the system comprising:

an acoustic transducer apparatus arranged to apply to the object acoustic vibration localised in two or three dimensions in a region in the object, the acoustic vibration comprising a carrier wave that is amplitude modulated by an AM waveform;

a transmitter arrangement arranged to illuminate the object with an illuminating electromagnetic wave having a frequency in a range extending down from 30 THz simultaneously with the application of acoustic vibration, the vibration direction of the acoustic vibration having a component parallel to the propagation direction of the illuminating electromagnetic wave so that so that the vibration of the object caused by the acoustic vibration in the region generates a scattered electromagnetic wave including a set of Doppler components shifted from the frequency of the illuminating electromagnetic wave by frequencies of the vibration of the object caused by the acoustic vibration and multiples thereof; and

a receiver arrangement arranged to receive the scattered electromagnetic wave generated in the region; and

a signal processing apparatus arranged to detect, from the received, scattered electromagnetic wave, a Doppler component shifted from the frequency of the illuminating electromagnetic wave by the frequency of a frequency component of the AM waveform, and to output a signal representing at least one characteristic of the detected Doppler component.

20. The system according to claim 19, wherein said AM waveform has a fundamental frequency component, said frequency component of the AM waveform being the fundamental frequency component.

21. The system according to claim 20, wherein said AM waveform further includes other frequency components.

22. The system according to claim 21, wherein the AM waveform is a square wave.

23. The system according to claim 19, wherein said frequency component of the AM waveform has a period of the same order of magnitude as the acoustic relaxation time of the region of the object.

24. The system according to claim 19, wherein said frequency component of the AM waveform is selected to provide a resonant vibration of the object.

25. The system according to claim 19, wherein the carrier wave has a frequency in the range from 10 kHz to 1 GHz.

26. The system according to claim 19, wherein said frequency component of the AM waveform is in the range from 1 Hz to 100 MHz.

27. The system according to claim 19, wherein the illuminating electromagnetic wave has an EM frequency in a range extending up from a value of twice said frequency component of the AM waveform, preferably ten times said frequency component of the AM waveform.

28. The system according to claim 19, wherein said object is a biological object.

29. The system according to claim 28, wherein said biological object is human or animal tissue.

30. The system according to claim 28, wherein the carrier wave has a frequency in a range extending up from 1 MHz and/or extending up to 50 MHz.

31. The system according to claim 28, wherein said frequency component of the AM waveform is in a range extending up from 10 kHz and/or extending up to 1 kHz.

32. The system according to claim 28, wherein the illuminating electromagnetic wave has a frequency in a range extending up to 2 GHz.

33. The system according to claim 19, wherein the signal processing apparatus comprises:

a phase locked-loop supplied with the received, scattered electromagnetic wave, the phase-locked loop being locked to the EM frequency and arranged to produce a frequency-demodulated signal comprising the set of the Doppler components frequency-demodulated from EM frequency; and

a lock-in amplifier supplied with the frequency-demodulated signal and configured to extract a signal at a reference frequency equal to the frequency of said frequency component of the AM waveform.

34. The system according to claim 33, wherein the lock-in amplifier is configured to generate the amplitude and/or phase of the extracted signal, being said at least one characteristic of the detected Doppler component.

35. The system according to claim 19, wherein:

the acoustic transducer apparatus is arranged to apply, simultaneously with said first mentioned acoustic vibration, a second acoustic vibration localised in two or three dimensions in a further region in the object, the second acoustic vibration comprising a carrier wave that is amplitude modulated by a second AM waveform;

the signal processing apparatus is also arranged to detect, from the received, scattered electromagnetic wave, a second Doppler component shifted from the frequency of the illuminating electromagnetic wave by the frequency of said frequency component of the second AM waveform, and to output a second signal representing at least one characteristic of the detected second Doppler component.

36. The system according to claim 19, being an imaging system, wherein:

the acoustic transducer apparatus is arranged to apply the acoustic vibration localised in a plurality of regions either sequentially or simultaneously but with AM waveforms including frequency components of different frequencies in each region;

the receiver apparatus is arranged to receive the scattered electromagnetic wave generated in each of the plurality of regions;

the signal processing apparatus is arranged to detect a Doppler component a Doppler component in respect of each region, to output a signal representing at least one characteristic of the detected Doppler component in respect of each region, and to store data representing the at least one characteristic of the detected Doppler component in respect of each region as image data.