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Electrochemical methods and devices for amending urine samples for immunosensor detection

  • US 9,488,663 B2
  • Filed: 03/12/2014
  • Issued: 11/08/2016
  • Est. Priority Date: 03/14/2013
  • Status: Active Grant
First Claim
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1. A device configured to perform an immunoassay for a target analyte in a urine sample, the device comprising:

  • a first region comprising reagents configured to amend the urine sample to remove or reduce a urea concentration in the urine sample below a preselected urea threshold, wherein the reagents comprise urease and a buffer; and

    a second region comprising at least one electrode coated with a biolayer comprising an attached capture antibody configured to bind to the target analyte, which is different from that of the urea, wherein the second region is configured to determine a concentration of the target analyte in the amended urine sample,wherein the first region is configured to provide a dissolved urease enzymatic activity within the amended urine sample in a range of about 10 to 10,000 IU/mL.

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