Analyte sensors comprising high-boiling point solvents

• US 9,492,112 B2
• Filed: 07/22/2015
• Issued: 11/15/2016
• Est. Priority Date: 11/24/2009
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Inventors: Bommakanti, Balasubrahmanya S., Sandhu, Gary, Hoss, Udo, McGarraugh, Geoffrey V.
• Primary Examiner(s): Kaur, Gurpreet
• Application Number:
  US14/806,424
• Publication Number:
  US 20150327801A1
• Time in Patent Office: 482 Days
• US Class Current: 1/1
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1473 invasive, e.g. introduced into the body by a catheter A61B5/1482 takes precedence
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
• CPC Class Codes: A61B 5/1495 Calibrating or testing of in-vivo probes
• CPC Class Codes: A61B 5/4839 combined with drug delivery
• CPC Class Codes: A61B 5/6847 mounted on an invasive device
• CPC Class Codes: A61B 5/742 using visual displays A61B5/339 takes precedence
• CPC Class Codes: A61B 5/746 Alarms related to a physiological condition, e.g. details of setting alarm thresholds or avoiding false alarms alarms per se G08B
• CPC Class Codes: A61K 38/28 Insulins
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

Generally, embodiments of the present disclosure relate to analyte determining methods and devices (e.g., electrochemical analyte monitoring systems) that have improved uniformity of distribution of the sensing layer by inclusion of a high-boiling point solvent, where the sensing layer is disposed proximate to a working electrode of in vivo and/or in vitro analyte sensors, e.g., continuous and/or automatic in vivo monitoring using analyte sensors and/or test strips. Also provided are systems and methods of using the, for example electrochemical, analyte sensors in analyte monitoring.

Claims

1. An electrode structure, comprising

a conductive material;

and

an enzyme formulation disposed on a surface of the conductive material, wherein the enzyme formulation comprises polymer, enzyme, and a high-boiling point solvent comprising ethylene glycol at a concentration of 0.5% to 5% (w/v) of the total formulation of the enzyme formulation.

2. The electrode structure of claim 1, wherein the enzyme formulation has a substantially arcuate profile as measured using a profilometer.

3. The electrode structure of claim 1, further comprising a membrane disposed over the enzyme formulation.

4. The electrode structure of claim 1, wherein the enzyme is a glucose-responsive enzyme.

5. The electrode structure of claim 1, wherein the enzyme formulation further comprises a redox mediator.

6. The electrode structure of claim 5, wherein the redox mediator comprises a ruthenium-containing complex or an osmium-containing complex.

7. The electrode structure of claim 5, wherein the redox mediator or the enzyme is covalently bonded to the polymer.

8. The electrode structure of claim 3, wherein the membrane is a polymeric membrane.

9. The electrode structure of claim 3, wherein the membrane limits the flux of analyte to the enzyme formulation.

10. The electrode structure of claim 3, wherein the membrane is covalently bonded to the enzyme formulation.

11. The electrode structure of claim 3, wherein the membrane is cross-linked to the enzyme formulation.