Methods of using regenerative cells to treat a burn
First Claim
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1. A method of treating a burn in a subject in need thereof, comprising:
- identifying a subject with a burn; and
administering a therapeutically effective amount of a concentrated population of adipose-derived regenerative cells comprising adipose-derived stem cells and endothelial progenitor cells to said subject sufficient to accelerate the healing of said burn wherein said concentrated population of adipose-derived regenerative cells is obtained by acquiring an adipose tissue sample, separating away adipocytes and connective tissue, and washing and concentrating said adipose-derived regenerative cells.
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Abstract
Cells present in adipose tissue are used to promote wound healing in a patient. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of regenerative cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the regenerative cells are not exposed to an external environment prior to being administered to a patient. Accordingly, in a preferred method, cells present in adipose tissue are placed directly into a recipient along with such additives necessary to promote, engender or support a therapeutic benefit.
178 Citations
23 Claims
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1. A method of treating a burn in a subject in need thereof, comprising:
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identifying a subject with a burn; and administering a therapeutically effective amount of a concentrated population of adipose-derived regenerative cells comprising adipose-derived stem cells and endothelial progenitor cells to said subject sufficient to accelerate the healing of said burn wherein said concentrated population of adipose-derived regenerative cells is obtained by acquiring an adipose tissue sample, separating away adipocytes and connective tissue, and washing and concentrating said adipose-derived regenerative cells.
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2. The method of claim 1, wherein the concentrated population of adipose-derived regenerative cells has not been cultured.
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3. The method of claim 1, wherein the concentrated population of adipose-derived regenerative cells is obtained from adipose tissue of the subject to whom the cells are administered.
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4. The method of claim 1, wherein said administering step comprises applying the concentrated population of adipose-derived regenerative cells directly to the burn.
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5. The method of claim 1, wherein said administering step comprises subcutaneous delivery.
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6. The method of claim 1, wherein said administering step comprises dermal delivery.
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7. The method of claim 1, wherein said administering step comprises intravascular delivery.
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8. The method of claim 1, wherein said administration step comprises administering multiple doses of said concentrated population of adipose-derived regenerative cells.
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9. The method of claim 1, further comprising:
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providing adipose tissue to a self-contained adipose tissue processing unit comprising; a tissue collecting container configured to receive and fully or partially disaggregate adipose tissue, wherein said tissue collecting container is coupled to a cell collection container wherein said coupling defines a closed system in which said fully or partially disaggregated adipose tissue directed from tissue collecting container to cell collection container is not exposed to the external environment; and
wherein said cell collection container, further comprises a cell concentrator that facilitates separation of the cells in a suspension; andan outlet that is structured to permit said concentrated population of adipose-derived regenerative cells comprising stem and progenitor to be removed from said adipose tissue processing unit; and obtaining the concentrated population adipose-derived regenerative cells from said self-contained adipose tissue processing unit.
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10. The method of claim 1, wherein the therapeutically effective amount of the concentrated population of adipose-derived regenerative cells comprises a minimum concentration of between 1×
- 105 and 1×
107 adipose-derived stem cells.
- 105 and 1×
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11. The method of claim 1, wherein the concentrated population of adipose-derived regenerative cells further comprises an additive.
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12. The method of claim 10, wherein said additive is selected from the group consisting of a cell differentiation factor, a growth promoter, an immunosuppressive agent, a medical device, or a combination thereof.
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13. The method of claim 10, wherein said additive is selected from the group consisting of other cells, tissue, and tissue fragments.
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14. The method of claim 10, wherein said additive comprises an angiogenic factor, or an arteriogenic factor.
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15. The method of claim 1, wherein said concentrated population of adipose-derived regenerative cells further comprises endothelial precursor cells.
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16. The method of claim 1, wherein said concentrated population of adipose-derived regenerative cells is applied to a biocompatible matrix.
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17. The method of claim 1, wherein said concentrated population of adipose-derived regenerative cells applied to a bandage.
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18. The method of claim 1, further comprising:
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performing excisional lipectomy to obtain adipose tissue from said subject; and processing said adipose tissue to obtain said concentrated population of adipose-derived regenerative cells.
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19. The method of claim 1, further comprising:
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performing lipoaspiration to obtain adipose tissue from said subject; and processing said adipose tissue to obtain said concentrated population of adipose-derived regenerative cells.
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20. The method of claim 1, wherein said concentrated population of adipose-derived regenerative cells is cryopreserved.
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21. The method of claim 16, wherein the biocompatible matrix comprises a synthetic or biological scaffold.
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22. The method of claim 1, wherein said concentrated population of adipose-derived cells is administered via an artificial or natural tissue scaffold.
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23. The method of claim 1, wherein said concentrated population of adipose-derived regenerative cells in not exposed to the external environment prior to the administration step.
Specification