FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
First Claim
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1. A method for integrating a nucleic acid sequence of interest into a specific site in the genome of a soybean cell, the method comprising:
- cleaving, in a site specific manner, a target site within any of SEQ ID NOs;
14 to 20 in a FAD2 gene in a soybean cell, to thereby generate a break in the FAD2 gene;
integrating into the break an exogenous nucleic acid sequence of interest.
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Abstract
Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.
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Citations
29 Claims
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1. A method for integrating a nucleic acid sequence of interest into a specific site in the genome of a soybean cell, the method comprising:
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cleaving, in a site specific manner, a target site within any of SEQ ID NOs;
14 to 20 in a FAD2 gene in a soybean cell, to thereby generate a break in the FAD2 gene;integrating into the break an exogenous nucleic acid sequence of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 11, 12, 13, 14, 15, 16)
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9. A soybean plant comprising a soybean cell modified by the following method:
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cleaving, in a site specific manner, a target site in a FAD2 gene in a soybean cell, to generate one double-stranded break within any of SEQ ID NOs;
14 to 20 in the FAD2 gene; andintegrating into the break an exogenous nucleic acid sequence of interest. - View Dependent Claims (10)
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17. A transgenic soybean cell comprising an exogenous nucleotide sequence of interest integrated within any of SEQ ID NOs:
- 14 to 20 of a FAD2 2.3 and/or FAD2 2.6 gene.
- View Dependent Claims (18, 19, 20, 21)
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22. A method of cleaving a FAD2 loci in a soybean cell, the method comprising:
introducing a fusion protein comprising a DNA-binding domain and a cleavage domain or cleavage half-domain into the cell, wherein the fusion protein binds with specificity to a target site within any of SEQ ID NOs;
14 to 20 in the FAD2 loci and cleaves to generate a break.- View Dependent Claims (23, 24, 25, 26, 27, 28, 29)
Specification