DNA polymerases
First Claim
Patent Images
1. A DNA polymerase mutant comprising SEQ ID NO:
- 13 with a mutation at position E189 and optionally one or more of the following mutations;
E230K, E76G, E76K, E76A, E90K, D551N, K206R, Q680R, L552R, H28R, E315K, L30R, L30P, D452N, G504S, G504D, G38R, F73V, H75R, A348V, L351F, A439T, L552R, I553V, D578N, H676R, D732G, E734G, E734K, F749V, E507K, E507A;
wherein the DNA polymerase mutant exhibits relative to wild-type DNA polymerase of SEQ ID NO;
13, and under the same conditions, increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor.
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Abstract
The invention provides novel mutant thermostable DNA polymerases having desirable properties such as increased polymerase efficiency and speed, increased affinity to DNA substrates and/or increased resistance to DNA polymerase inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel DNA polymerases.
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Citations
23 Claims
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1. A DNA polymerase mutant comprising SEQ ID NO:
- 13 with a mutation at position E189 and optionally one or more of the following mutations;
E230K, E76G, E76K, E76A, E90K, D551N, K206R, Q680R, L552R, H28R, E315K, L30R, L30P, D452N, G504S, G504D, G38R, F73V, H75R, A348V, L351F, A439T, L552R, I553V, D578N, H676R, D732G, E734G, E734K, F749V, E507K, E507A;
wherein the DNA polymerase mutant exhibits relative to wild-type DNA polymerase of SEQ ID NO;
13, and under the same conditions, increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
- 13 with a mutation at position E189 and optionally one or more of the following mutations;
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22. A DNA polymerase mutant of SEQ ID NO:
- 13 comprising a mutation at position E189 and optionally one or more of the following mutations;
E230K, E76G, E76K, E76A, E90K, D551N, K206R, Q680R, L552R, H28R, E315K, L30R, L30P, D452N, G504S, G504D, G38R, F73V, H75R, A348V, L351F, A439T, L552R, I553V, D578N, H676R, D732G, E734G, E734K, F749V, E507K, E507A;
wherein the DNA polymerase mutant exhibits relative to wild-type DNA polymerase of SEQ ID NO;
13, and under the same conditions, increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor.
- 13 comprising a mutation at position E189 and optionally one or more of the following mutations;
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23. A DNA polymerase mutant comprising SEQ ID NO:
- 13 comprising a mutation at position E189 and optionally one or more of the following mutations;
E230K, E76G, E76K, E76A, E90K, D551N, K206R, Q680R, L552R, H28R, E315K, L30R, L30P, D452N, G504S, G504D, G38R, F73V, H75R, A348V, L351F, A439T, L552R, I553V, D578N, H676R, D732G, E734G, E734K, F749V, E507K, E507A;
wherein the DNA polymerase mutant exhibits relative to wild-type DNA polymerase of SEQ ID NO;
13, and under the same conditions, increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor, and wherein the DNA polymerase mutant has at least 2 and no more than 12 mutations of SEQ ID NO;
13.
- 13 comprising a mutation at position E189 and optionally one or more of the following mutations;
Specification