Plastic particle detector for detection of biological aerosol and other fluorescent materials

• US 9,500,591 B1
• Filed: 04/03/2015
• Issued: 11/22/2016
• Est. Priority Date: 04/03/2015
• Status: Active Grant

• Current Assignee: Government of the United States of America
• Original Assignee: The United States Of America As Represented By The Secretary Of The Army
• Inventors: Goad, Aime P., Narayanan, Fiona E., Kilper, Gary K., Wong, Anna, Sickenberger, David W., Kreis, Richard J., Strauch, Lester D. III, Cabalo, Jerry B., Wylie, Harold S.
• Primary Examiner(s): Taningco, Marcus
• Application Number:
  US14/678,395
• Time in Patent Office: 599 Days
• US Class Current: 1/1
• CPC Class Codes: B29K 2077/00 Use of PA, i.e. polyamides, e.g. polyesteramides or derivatives thereof, as moulding material
• CPC Class Codes: B29K 2507/04 Carbon
• CPC Class Codes: B29K 2509/08 Glass
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

A plastic particle detector for detecting biological and other fluorescent materials is disclosed. The detector detects the fluorescence and scattering signals from these materials using deep UV excitation. The detector is fabricated using plastic materials and exploits the properties of lower manufacturing costs, lower materials costs, light weight, ruggedness and assembly ease offered by plastics, while eliminating stray fluorescence signals ordinarily generated by plastic materials.

Claims

1. A particle detector, comprising:

a excitation region, comprising a first plastic housing and an excitation source for generating light to excite particles passing though the particle detector;

a interrogation region for producing an emission beam from one or more particles excited by light from the excitation source, comprising a second plastic housing; and

a detection region for receiving the emission beam, comprising a third plastic housing and one or more optical detectors configured to determine light scattering and fluorescence properties of particles, and

where the plastic in said first, second, and third plastic housings is a polyamide 66 resin with glass and carbon black fill.

2. The particle detector of claim 1, wherein the first, second and third plastic housings are formed from an uncoated plastic that does not interact with light from the excitation source to produce fluorescent light beams or scattering light beams that will be detected by the one or more optical detectors.

3. The particle detector of claim 1, wherein the first, second and third plastic housings are injection-molded.

4. The particle detector of claim 1, wherein aid first, second and third plastic housings include embedded fasteners so that the housings can be assembled to form the particle detector without additional hardware, glue or welding.

5. The particle detector of claim 1, wherein said excitation region further comprises:

one or more lenses to collect light from the excitation source fitted into said first housing adjacent to the excitation source; and

one or more filters fitted into the opposite end of the first housing from the excitation source to remove stray light from the excitation source.

6. The particle detector of claim 1, wherein said interrogation region further comprises:

a cavity within said second housing, said first housing operatively coupled to one side of said second housing and said third housing operatively coupled to said second housing at a position perpendicular to said first housing; and

a beam dump operatively coupled to said second housing opposite said first housing.

7. The particle detector of claim 6, wherein said second housing further comprises:

embedded attachment points that mate with those on the first and third housing;

embedded attachment points that mate with those on inlet and exhaust pitot tubes on opposite sides of said cavity; and

embedded attachment points that mate with those on the beam dump.

8. The particle detector of claim 1, wherein the detection region further comprises:

a beam splitter for splitting the emission beam into two portions;

a first optical detector configured to determine radiation scattering properties of particles passing through the particle detector according to a first portion of the emission beam; and

a second optical detector configured to determine fluorescene properties of the particles passing through the particle detector according to a second portion of the emission beam.

9. The particle detector of claim 1, wherein the one or more optical detectors comprise photomultipliers.

10. The particle detector of claim 1, wherein the one or more optical detectors comprise avalanche photodiodes.

11. The particle detector of claim 1, wherein the excitation source generates light in the deep UV wavelength range.

12. A particle detector, comprising:

a excitation region, comprising;

a first plastic housing;

an excitation source in one end of said housing;

one or more lenses to collect light from the excitation source fitted into said first housing adjacent to the excitation source; and

one or more filters fitted into the opposite end of the first housing from the excitation source to remove stray light;

an interrogation region, comprising;

a second plastic housing operatively coupled to said first housing; and

a cavity in which one or more particles are excited by light from the excitation source; and

a detection region, comprising;

a third plastic housing operatively coupled to the second housing perpendicularly to said first housing;

a first optical detector configured to determine radiation scattering properties of particles passing through the particle detector; and

a second optical detector configured to determine fluorescence properties of the particles passing through the particle detector, and

wherein the plastic in said first, second, and third plastic housings is a polyamide 66 resin with glass and carbon black fill.

13. The particle detector of claim 12, further comprising a beam dump operatively coupled to said second housing opposite said first housing.

14. The particle detector of claim 12, wherein the first, second and third plastic housings are formed from uncoated plastic that does not interact with light from the excitation source to produce fluorescent light beams or scattering light beams that will be detected by the one or more optical detectors.

15. The particle detector of claim 12, wherein the fast, second and third plastic housings are injection-molded.

16. The particle detector of claim 12, wherein said first, second and third plastic housings contain embedded fasteners so that the housings can be assembled to form the particle detector without additional hardware, glue or welding.

17. The particle detector of claim 12, wherein the emission source generates light in the deep UV wavelength range.

18. A method of manufacturing a plastic particle detector, comprising the steps of:

forming a first housing from plastic, said first housing including molded structures to hold an excitation source and one or more optical devices for focusing light from the excitation source on a particle;

forming a second housing from plastic, said second housing including molded structures wherein said focused light from said excitation source intersects with said particle and generates an emission beam, said first housing operatively coupled to one side of said second housing; and

forming a third housing from plastic, said third housing operatively coupled to said second housing perpendicularly to said first housing, said third housing receiving the emission beam and including molded structures for attaching two or more optical detectors; and

wherein said first, second and third plastic housings comprise a polyamide 66 resin with glass and carbon black fill and contain embedded fasteners so that the housings can be assembled to form the particle detector without additional hardware, glue or welding.

19. The method of claim 18, further comprising the step of:

forming a beam dump from plastic, said beam dump operatively coupled to said cavity opposite said first housing, without requiring the use of additional fasteners.

20. The method of claim 18, wherein the first, second and third plastic housings are formed from uncoated plastic that does not interact with light from the excitation source to produce fluorescent light beams or scattering light beams that will be detected by the one or morn optical detectors.

21. The method of claim 18, wherein the first, second and third plastic housings we injection-molded.

22. The method of claim 18, where the excitation source generates light in the deep UV wavelength range.

23. The method of claim 18, wherein the one or more optical detectors comprise photomultipliers.

24. The method of claim 18, wherein the one or more optical detectors comprise avalanche photodiodes.