Systems and methods for controlling temperature of small volumes

• US 9,500,610 B2
• Filed: 11/06/2013
• Issued: 11/22/2016
• Est. Priority Date: 11/06/2012
• Status: Active Grant

• Current Assignee: The United States of America As Represented By The Secretary of Commerce
• Original Assignee: The United States of America As Represented By The Secretary of Commerce
• Inventors: Kasianowicz, John J., Reiner, Joseph E., Balijepalli, Arvind K., Robertson, Joseph W., Burden, Daniel L., Burden, Lisa
• Primary Examiner(s): Verbitsky, Gail Kaplan
• Application Number:
  US14/073,126
• Publication Number:
  US 20140064324A1
• Time in Patent Office: 1,112 Days
• Field of Search: 374/183, 374/44, 374/45, 374/100, 374/178, 374/121, 374/120, 977/700, 977/742, 977/788, 977/902, 438/478
• US Class Current: 1/1
• CPC Class Codes: G01K 2211/00 Thermometers based on nanotechnology
• CPC Class Codes: G01K 7/16 using resistive elements resistive elements per se H01C, H01L
• CPC Class Codes: G01N 25/00 Investigating or analyzing materials by the use of thermal means G01N3/00 - G01N23/00 take precedence

Abstract

Systems and methods for controlling the temperature of small volumes such as yoctoliter volumes, are described. The systems include one or more plasmonic nanostructures attached at or near a nanopore. Upon excitation of the plasmonic nanostructures, such as for example by exposure to laser light, the nanoparticles are rapidly heated thereby causing a change in the ionic conductance along the nanopore. The temperature change is determined from the ionic conductance. These temperature changes can be used to control rapid thermodynamic changes in molecular analytes as they interact with the nanopore.

Claims

1. A system for measuring temperature at a nanopore, the system comprising:

a substrate defining a surface and at least one nanopore;

a plasmonic structure disposed proximate the nanopore;

an ionic conducting solution which bathes the nanopore and the plasmonic structure;

a light source capable of emitting light of sufficient intensity and wavelength to excite the plasmonic structure;

an ionic current measuring assembly configured to measure changes in ionic conductance proximate to the nanopore;

whereby upon excitation of the plasmonic structure resulting from emission of light from the light source, changes in ionic conductance measured by the ionic current measuring assembly are used to determine temperature or temperature changes at the nanopore.

2. The system of claim 1 where the substrate includes a biological layer disposed on the surface of the substrate, the biological layer defining a second surface and at least one nanopore, and the plasmonic structure including one or more metallic nanoparticles tethered to the second surface of the biological layer.

3. The system of claim 2 wherein the nanoparticles have a size within a range of from about 10 nm to about 1,000 nm.

4. The system of claim 2 wherein the metallic nanoparticles are tethered to the biological layer by at least one oligomer.

5. The system of claim 4 wherein the oligomer is an oligonucleotide having from 10 to 500 repeating units.

6. The system of claim 1 wherein the light source is selected from the group consisting of a laser, an incandescent light source, a light emitting diode, and an arc lamp.

7. The system of claim 1 wherein the ionic conducting solution is an electrolyte solution.

8. The system of claim 1 wherein the ionic conducting solution is an ionic liquid.

9. A method for measuring temperature at a nanopore, the method comprising:

providing a plasmonic structure;

affixing the plasmonic structure proximate the nanopore;

emitting light of sufficient intensity and wavelength to excite the plasmonic structure and induce a change in temperature;

measuring changes in ionic conductance proximate the nanopore;

whereby the changes in ionic conductance are used to determine temperature or temperature changes at the nanopore.

10. The method of claim 9 wherein during emitting of the light, the light is absorbed at or near the surface plasmon resonance of the plasmonic structure and increases the temperature of the plasmonic structure and the heat is conducted to the ionic conducting solution.

11. The method of claim 9 wherein the plasmonic structures include metallic nanoparticles.

12. The method of claim 11 wherein the metallic nanoparticles have a size within a range of from about 10 nm to about 1,000 nm.

13. The method of claim 9 wherein the emitting light is performed using a light source selected from the group consisting of a laser, an incandescent light source, a light emitting diode, and an arc lamp.

14. The method of claim 9 wherein the plasmonic structure includes metallic nanoparticles and the nanopore is defined in a biological layer, wherein affixing is performed by attaching the metallic nanoparticles to the biological layer using at least one oligomer.

15. The method of claim 14 wherein the oligomer is an oligonucleotide having from 10 to 500 repeating units.