Process for realising a device for collecting and transferring samples for molecular biology
First Claim
1. A method for collecting, conserving and transferring samples of DNA or RNA for molecular biology, the method comprising:
- collecting a sample of DNA or RNA for molecular biology on at least a flocked collecting portion (3) of a collecting device (1) having a support body (2), the collecting portion (3) comprising a first portion (2a) of the support body (2) and a plurality of fibres (6) attached and arranged on the first portion (2a) of the support body (2) by flocking;
conserving the integrity of the DNA or RNA samples collected by the device sufficiently to enable subsequent use of said samples in laboratory examinations of analytic or diagnostic type, wherein the sample is conserved and preserved on the collecting portion for a predetermined period of time of at least twenty-four hours; and
releasing the sample of DNA or RNA for molecular biology from the collecting portion (3) in order to enable performance of operation of molecular biology on the sample; and
wherein conserving comprises pretreating the plurality of fibres (6) with a surfactant, wherein the surfactant is a mixture of chlorides of alkyl-benzyl-dimethyl ammonium, in which the alkyl group varies from octile (C8H17—
) to octadecyl (C18H37—
), wherein the pre-treated plurality of fibers (6) conserve the integrity of the DNA or RNA samples collected by the device on said collecting portion during said predetermined period of time.
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Accused Products
Abstract
A process for realizing a device (1) for collecting and transferring samples for molecular biology, the device including at least: a support body (2) having at least a first portion (2a), and a plurality of fibers (6) attached and arranged on the first portion (2a) of the support body (2) by flocking, such as to define a flocked collecting portion (3) destined to collect, on the said collecting portion, (3) a quantity of the sample for molecular biology, the process including at least a step of pre-treating the plurality of fibers (6) with a surfactant, the step of pre-treating the plurality of fibers (6) with a surfactant being performed after application of the fibers on the support body (2) by flocking and/or before application of the fibers (6) on the body (2) by flocking and/or wherein the surfactant is added to the fiber (6) during production of the fiber (6).
123 Citations
17 Claims
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1. A method for collecting, conserving and transferring samples of DNA or RNA for molecular biology, the method comprising:
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collecting a sample of DNA or RNA for molecular biology on at least a flocked collecting portion (3) of a collecting device (1) having a support body (2), the collecting portion (3) comprising a first portion (2a) of the support body (2) and a plurality of fibres (6) attached and arranged on the first portion (2a) of the support body (2) by flocking; conserving the integrity of the DNA or RNA samples collected by the device sufficiently to enable subsequent use of said samples in laboratory examinations of analytic or diagnostic type, wherein the sample is conserved and preserved on the collecting portion for a predetermined period of time of at least twenty-four hours; and releasing the sample of DNA or RNA for molecular biology from the collecting portion (3) in order to enable performance of operation of molecular biology on the sample; and wherein conserving comprises pretreating the plurality of fibres (6) with a surfactant, wherein the surfactant is a mixture of chlorides of alkyl-benzyl-dimethyl ammonium, in which the alkyl group varies from octile (C8H17—
) to octadecyl (C18H37—
), wherein the pre-treated plurality of fibers (6) conserve the integrity of the DNA or RNA samples collected by the device on said collecting portion during said predetermined period of time. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for collecting, conserving and transferring samples of DNA or RNA for molecular biology, the method comprising:
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collecting a sample of DNA or RNA for molecular biology on at least a flocked collecting portion (3) of a collecting device (1) having a support body (2), the collecting portion (3) comprising a first portion (2a) of the support body (2) and a plurality of fibres (6) attached and arranged on the first portion (2a) of the support body (2) by flocking, said collecting portion (3) exhibiting a surface density of the fibres (6) on the collecting portion (3) of between 50 and 500 fibres per mm2 or between 100 and 200 fibres per mm2; conserving the integrity of the DNA or RNA samples collected by the device sufficiently to enable subsequent use of said samples in laboratory examinations of analytic or diagnostic type, wherein the sample is conserved and preserved on the collecting portion for a predetermined period of time of at least twenty-four hours; and releasing the sample of DNA or RNA for molecular biology from the collecting portion (3) in order to enable performance of operation of molecular biology on the sample; and wherein conserving comprises pretreating the plurality of fibres (6) with a surfactant, wherein the pre-treated plurality of fibers (6) conserve the integrity of the DNA or RNA samples collected by the device on said collecting portion during said predetermined period of time.
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17. A method for collecting, conserving and transferring samples of DNA or RNA for molecular biology, the method comprising:
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collecting a sample of DNA or RNA for molecular biology on at least a flocked collecting portion (3) of a collecting device (1) having a support body (2), the collecting portion (3) comprising a first portion (2a) of the support body (2) and a plurality of fibres (6) attached and arranged on the first portion (2a) of the support body (2) by flocking; conserving the integrity of the DNA or RNA samples collected by the device sufficiently to enable subsequent use of said samples in laboratory examinations of analytic or diagnostic type, wherein the sample is conserved and preserved on the collecting portion for a predetermined period of time of at least twenty-four hours; and releasing the sample of DNA or RNA for molecular biology from the collecting portion (3) in order to enable performance of operation of molecular biology on the sample; and wherein conserving comprises pretreating the plurality of fibres (6) with a surfactant, wherein the pre-treated plurality of fibers (6) conserve the integrity of the DNA or RNA samples collected by the device on said collecting portion during said predetermined period of time; further comprising the step of drying at least the collecting portion (3) provided with the sample, wherein the step of drying is performed by drying in a forced-ventilation oven.
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Specification