Methods of using adipose derived regenerative cells to promote restoration of intevertebral disc

• US 9,504,716 B2
• Filed: 08/09/2010
• Issued: 11/29/2016
• Est. Priority Date: 12/07/2001
• Status: Expired due to Fees

• Current Assignee: Lorem Vascular Pte. Ltd.
• Original Assignee: Cytori Therapeutics Incorporated
• Inventors: Hedrick, Marc H., Fraser, John K., Riley, Susan Lynn, Schreiber, Ronda Elizabeth
• Primary Examiner(s): Lankford, Blaine
• Application Number:
  US12/853,110
• Publication Number:
  US 20100303774A1
• Time in Patent Office: 2,304 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61L 2300/40 characterised by a specific therapeutic activity or mode of action
• CPC Class Codes: A61L 2300/412 Tissue-regenerating or healing or proliferative agents
• CPC Class Codes: A61L 2300/64 Animal cells
• CPC Class Codes: A61L 2400/06 Flowable or injectable implant compositions
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 2430/40 Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/3608 Bone, e.g. demineralised bone matrix [DBM], bone powder
• CPC Class Codes: A61L 27/365 Bones
• CPC Class Codes: A61L 27/3654 Cartilage, e.g. meniscus
• CPC Class Codes: A61L 27/3691 characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61L 27/3847 Bones
• CPC Class Codes: A61L 27/3852 Cartilage, e.g. meniscus
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61L 27/52 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61P 19/02 : for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 19/04 : for non-specific disorders of the connective tissue
• CPC Class Codes: A61P 19/08 : for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: C12N 5/0667 : Adipose-derived stem cells [ADSC]; Adipose stromal stem cells

Abstract

Regenerative cells present in adipose tissue are used to treat patients, including patients with musculoskeletal diseases or disorders. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of regenerative cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Accordingly, in a preferred method, regenerative cells present in adipose tissue are placed directly into a recipient along with such additives necessary to promote, engender or support a therapeutic musculoskeletal benefit.

Claims

1. A method for promoting restoration of an intervertebral disc in a subject comprising:

identifying a subject in need of intervertebral disc restoration;

obtaining a unit of adipose tissue from said subject;

processing said unit of adipose tissue obtained from said subject by separating adipose-derived cells comprising stem and progenitor cells from mature adipocytes and connective tissue, and washing, separating and concentration said adipose-derived cells to obtain a concentrated population of cells that comprises stem cells and endothelial progenitor cells; and

administering said concentrated population of cells to the intervertebral disc of said subject without culturing the concentrated population of cells prior to administration to the subject.

2. The method of claim 1, wherein the concentrated population of cells further comprises a hydrogel liquid.

3. The method of claim 1, wherein said concentrated population of cells is administered by direct injection into the intervertebral disc.

4. The method of claim 2, wherein said concentrated population of cells is administered by injection into a gel that is placed within said subject.

5. The method of claim 1, wherein said subject is identified as being in need of an intervertebral body spinal fusion.

6. The method of claim 1, wherein said subject is identified as being in need of an intertransverse process spinal fusion.

7. The method of claim 1, wherein one or more additives are added to the concentrated population of cells.

8. The method of claim 7, wherein the one or more additives are growth factors, re-suspension fluids, cells, tissue, or tissue fragments or combinations thereof.

9. The method of claim 1, wherein the concentrated population of cells are placed in a gel.

10. The method of claim 1, wherein the administration comprises injecting the concentrated population of cells into the nucleus pulposis.

11. The method of claim 1, wherein the concentrated population of cells is combined with a biocompatible matrix.

12. The method of claim 11, wherein the biocompatible matrix is a resorbable scaffold.

13. The method of claim 1, wherein the subject has degenerative disc disease.

14. The method of claim 1, wherein the intervertebral disc is ruptured.

15. The method of claim 1, wherein the subject has chronic inflammation of the intervertebral disc.

16. The method of claim 1, wherein the intervertebral disc comprises a localized disc herniation with a contained or escaped extrusion.

17. The method of claim 1, wherein the concentrated population of adipose-derived cells is cryopreserved.