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Device and method for amplifying target nucleic acid

  • US 9,506,105 B2
  • Filed: 08/11/2014
  • Issued: 11/29/2016
  • Est. Priority Date: 04/17/1997
  • Status: Expired due to Fees
First Claim
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1. A method for amplifying a target nucleic acid present in a sample, the method comprising:

  • introducing, via an entrance opening of a planar fluidic assembly, a sample containing one or more target nucleic acids into a flow channel of the planar fluidic assembly, the flow channel extending from the entrance opening to an exit vent, the flow channel having a substantially uniform cross-section from the entrance opening to the exit vent, wherein a plurality of nucleic acid primers that are complementary to a portion of the one or more target nucleic acids are preloaded and retained at locations within and along the flow channel from the entrance opening to the exit vent;

    subjecting the sample introduced into the flow channel to a primer-based amplification reaction using the nucleic acid primers, wherein the primer-based amplification reaction produces amplified product of the one or more target nucleic acids;

    retaining the amplified product at one or more of the locations within the flow channel during the primer-based amplification reaction; and

    detecting localized accumulation of the retained amplified product, the localized accumulation occurring at the one or more locations.

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