Method for controlling substance moving speed and apparatus for controlling the same

• US 9,506,894 B2
• Filed: 08/26/2013
• Issued: 11/29/2016
• Est. Priority Date: 12/27/2012
• Status: Active Grant

• Current Assignee: Osaka University
• Original Assignee: Quantum Biosystems Inc.
• Inventors: Kawai, Tomoji, Ryuzaki, Soh, Taniguchi, Masateru
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US13/975,610
• Publication Number:
  US 20140183040A1
• Time in Patent Office: 1,191 Days
• US Class Current: 1/1
• CPC Class Codes: G01N 27/44752 Controlling the zeta potential, e.g. by wall coatings
• CPC Class Codes: G01N 27/44756 Apparatus specially adapted therefor
• CPC Class Codes: G01N 33/48721 Investigating individual macromolecules, e.g. by translocation through nanopores Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68

Abstract

The present invention provides a method and apparatus for controlling the moving speed of a substance, both of which can adjust the moving speed of a substance to a desired speed. The control method and control apparatus cause a substance to pass through an internal space, in which an electro-osmotic flow is generated, of a surround electrode formed so as to surround part of the moving path of the substance, whereby the control method and control apparatus change the moving speed of the substance.

Claims

1. A method for regulating a flow rate of a biomolecule through a fluid flow path, comprising:

(a) subjecting a solution comprising said biomolecule to flow through said fluid flow path having a length using an electric field provided by a pair of electrodes at opposing ends of said fluid flow path, wherein said fluid flow path includes an internal space comprising different electrode sections that substantially oppose one another along said length, wherein each of said different electrode sections includes a non-conductive film adjacent to a conductive material, which non-conductive film is in contact with said solution;

(b) changing said flow rate of said biomolecule through said fluid flow path by applying a positive or negative voltage to said different electrode sections; and

(c) detecting said biomolecule or a portion thereof upon changing said flow rate of said biomolecule through said fluid flow path.

2. The method of claim 1, wherein said solution includes an electrolyte.

3. The method of claim 1, wherein said biomolecule is charged.

4. The method of claim 1, wherein (b) comprises supplying a gate voltage from about −

3 V to 3 V to said different electrode sections.

5. The method of claim 1, wherein said fluid flow path has a first width (Y) and said biomolecule in said fluid flow path has a second width (X), and wherein 0.5<

X/Y<

1.

6. The method of claim 5, wherein 0.7<

X/Y<

1.

7. The method of claim 6, wherein 0.8<

X/Y<

1.

8. The method of claim 1, wherein applying said positive or negative voltage to said different electrode sections generates electro-osmotic flow in said internal space.

9. The method of claim 1, wherein each of said different electrode sections is formed of a metallic material.

10. The method of claim 1, wherein said non-conductive film is a semiconductor oxide film.

11. The method of claim 1, wherein said fluid flow path includes a layer of charge adjacent to said different electrode sections having a Debye length.

12. The method of claim 1, wherein said biomolecule is a nucleic acid molecule.

13. The method of claim 1, wherein said biomolecule is a protein.

14. The method of claim 1, wherein said pair of electrodes includes a first electrode and a second electrode, and wherein (b) comprises applying said positive or negative voltage to said first electrode.

15. The method of claim 1, wherein said conductive material is covered by said non-conductive film.

16. A system for regulating a flow rate of a biomolecule through a fluid flow path, comprising:

(a) a pair of electrodes that provide an electric field to subject a solution comprising said biomolecule to flow;

(b) a fluid flow path having a length and with a cross-section that is sufficient to permit said solution having said biomolecule to flow therethrough, wherein said pair of electrodes is at opposing ends of said fluid flow path, and wherein said fluid flow path includes an internal space comprising different electrode sections that substantially oppose one another along said length, wherein each of said different electrode sections includes a non-conductive film adjacent to a conductive material, which non-conductive film is in contact with said solution;

(c) a voltage application member that applies a positive or negative voltage to said different electrode sections to change said flow rate of said biomolecule through said fluid flow path; and

(d) a detector that detects said biomolecule or a portion thereof upon changing said flow rate of said biomolecule through said fluid flow path.

17. The system of claim 16, wherein said solution includes an electrolyte.

18. The system of claim 16, wherein said biomolecule is charged.

19. The system of claim 16, wherein voltage application member supplies a gate voltage from about −

3 V to 3 V to said different electrode sections.

20. The system of claim 16, wherein said fluid flow path has a first width (Y) and said biomolecule in said fluid flow path has a second width (X), and wherein 0.5<

X/Y<

1.

21. The system of claim 20, wherein 0.7<

X/Y<

1.

22. The system of claim 21, wherein 0.8<

X/Y<

1.

23. The system of claim 16, wherein said non-conductive film is a semiconductor oxide film.

24. The system of claim 16, wherein said fluid flow path includes a layer of charge adjacent to said different electrode sections having a Debye length.

25. The system of claim 16, wherein said application of said positive or negative voltage to said different electrode sections generates electro-osmotic flow in said internal space.

26. The system of claim 16, wherein said biomolecule is a nucleic acid molecule.

27. The system of claim 16, wherein said biomolecule is a protein.

28. The system of claim 16, wherein said pair of electrodes includes a first electrode and a second electrode, and wherein said voltage application member applies said positive or negative voltage to said first electrode.

29. The system of claim 16, wherein said conductive material is covered by said non-conductive film.