Stable VEGF antagonist formulations
First Claim
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1. A stable liquid formulation of a vascular endothelial growth factor (VEGF)-specific antagonist, comprising:
- 10-100 mg/ml of a fusion protein comprising a receptor component, which comprises an immunoglobulin-like (Ig) domain 2 of a first VEGF receptor and an Ig domain 3 of a second VEGF receptor, and a multimerizing component;
1-10 mM phosphate buffer;
1-10 mM citrate buffer;
25-150 mM NaCl; and
5-30% sucrose, at a pH of about 6-6.5.
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Abstract
Formulations of a vascular endothelial growth factor (VEGF)-specific fusion protein antagonist are provided including a pre-lyophilized formulation, a reconstituted lyophilized formulation, and a stable liquid formulation. Preferably, the fusion protein has the sequence of SEQ ID NO:4.
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8 Claims
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1. A stable liquid formulation of a vascular endothelial growth factor (VEGF)-specific antagonist, comprising:
- 10-100 mg/ml of a fusion protein comprising a receptor component, which comprises an immunoglobulin-like (Ig) domain 2 of a first VEGF receptor and an Ig domain 3 of a second VEGF receptor, and a multimerizing component;
1-10 mM phosphate buffer;
1-10 mM citrate buffer;
25-150 mM NaCl; and
5-30% sucrose, at a pH of about 6-6.5. - View Dependent Claims (2, 3, 4, 5, 6)
- 10-100 mg/ml of a fusion protein comprising a receptor component, which comprises an immunoglobulin-like (Ig) domain 2 of a first VEGF receptor and an Ig domain 3 of a second VEGF receptor, and a multimerizing component;
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7. A stable liquid formulation of a VEGF-specific antagonist, comprising:
- about 25 mg/mL of a homodimer consisting essentially of amino acids 27-457 of SEQ ID NO;
4;
about 5 mM phosphate buffer;
about 5 mM citrate buffer;
about 20% sucrose;
about 100 mM NaCl; and
about 0.1% polysorbate 20, at a pH of about 6.2. - View Dependent Claims (8)
- about 25 mg/mL of a homodimer consisting essentially of amino acids 27-457 of SEQ ID NO;
Specification