Droplet actuator with improved top substrate

• US 9,511,369 B2
• Filed: 04/09/2014
• Issued: 12/06/2016
• Est. Priority Date: 09/04/2007
• Status: Active Grant

• Current Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Original Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Inventors: Srinivasan, Vijay, Pollack, Michael G., Shenderov, Alexander, Hua, Zhishan, Sudarsan, Arjun
• Primary Examiner(s): Dieterle, Jennifer
• Application Number:
  US14/248,884
• Publication Number:
  US 20140216932A1
• Time in Patent Office: 972 Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension
• CPC Class Codes: Y10T 29/49826 Assembling or joining

Abstract

The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.

Claims

1. A droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein:

(a) the base substrate comprises electrodes configured for conducting droplet operations in the gap; and

(b) the top substrate comprises a glass substrate portion coupled to a non-glass portion, where the non-glass portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap.

2. The droplet actuator of claim 1 wherein the non-glass portion comprises a plastic or resin portion.

3. The droplet actuator of claim 1 wherein the non-glass portion comprises a portion into which the glass substrate portion is inserted.

4. The droplet actuator of claim 1 wherein the fluid path is arranged to flow fluid into an actual or virtual reservoir associated with one or more reservoir electrodes associated with the base substrate.

5. The droplet actuator of claim 1 wherein the fluid path is arranged to flow fluid into proximity with one or more of the electrodes.

6. The droplet actuator of claim 1 wherein the glass substrate portion does not include openings therein.

7. The droplet actuator of claim 1 wherein:

(a) the non-glass portion overlaps the glass substrate portion; and

(b) an aperture is provided in the non-glass portion for providing a sensing path from the gap, through the glass substrate portion, through the aperture to an exterior of the droplet actuator.

8. The droplet actuator of claim 7 further comprising a fitting provided in association with the aperture for fitting a sensor onto the droplet actuator.

9. The droplet actuator of claim 7 further comprising a handle extending from the glass substrate portion and arranged to facilitate user handling of the droplet actuator.

10. The droplet actuator of claim 1 wherein the non-glass portion further comprises a hinged cover arranged to seal the openings when the hinged cover is in a closed position.

11. The droplet actuator of claim 10 wherein the hinged cover comprises one or more dried reagents associated therewith, such that when fluid is present in one or more of the openings, and the cover is closed, the dried reagents contact the fluid and are combined therewith to form fluid reagents.

12. The droplet actuator of claim 1 wherein:

(a) the non-glass portion overlaps the glass substrate portion; and

(b) one or more of the openings extends through the non-glass portion, through the glass substrate portion, and into the gap.

13. The droplet actuator of claim 12 wherein the opening extending through the non-glass portion is configured as a fluid reservoir.

14. A method of loading a fluid onto a droplet actuator, the method comprising:

(a) providing a droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein;

(i) the base substrate comprises electrodes configured for conducting droplet operations in the gap; and

(ii) the top substrate comprises a glass substrate portion coupled to a non-glass portion, where the non-glass portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap; and

(b) loading a fluid through the opening and into the gap.

15. A method of assembling a droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein the base substrate comprises electrodes configured for conducting droplet operations in the gap, and the top substrate comprises a glass substrate portion coupled to a non-glass portion, where the non-glass portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap, the method comprising:

(a) coupling the glass substrate portion to the non-glass portion; and

(b) assembling the top substrate with the bottom substrate to form a gap therebetween suitable for conducting droplet operations.

16. A method of conducting a droplet operation, the method comprising:

(a) providing a droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein;

(i) the base substrate comprises electrodes configured for conducting droplet operations in the gap; and

(ii) the top substrate comprises a glass substrate portion coupled to a non-glass portion, where the non-glass portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap; and

(b) loading a liquid onto the droplet actuator into proximity with one or more electrodes; and

(c) using the one or more electrodes to conduct the droplet operation.