Modified native beta-ketoacyl-ACP synthases and engineered microorganisms
First Claim
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1. A variant β
- -ketoacyl-ACP synthase (FabB) having at least 85% amino acid sequence identity to SEQ ID NO;
2, wherein said variant FabB comprises an amino acid substitution at I231, wherein the position is numbered with reference to SEQ ID NO;
2.
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Abstract
Genetically engineered cells and microorganisms are provided that produce fatty alcohols and fatty acids. In particular, engineered microbial cells comprise a modified native gene having β-ketoacyl-acp synthase activity.
41 Citations
31 Claims
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1. A variant β
- -ketoacyl-ACP synthase (FabB) having at least 85% amino acid sequence identity to SEQ ID NO;
2, wherein said variant FabB comprises an amino acid substitution at I231, wherein the position is numbered with reference to SEQ ID NO;
2. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 26, 27, 28, 29, 30, 31)
- -ketoacyl-ACP synthase (FabB) having at least 85% amino acid sequence identity to SEQ ID NO;
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22. A recombinant cell culture comprising engineered bacterial cells, said engineered bacterial cells comprising (a) a modified native gene encoding variant FabB having an amino acid sequence 85% identical to SEQ ID NO:
- 2 wherein the amino acid residue corresponding to I231 of SEQ ID BNO;
2 is substituted and (b) one or more heterologous polynucleotides encoding a fatty alcohol reductase and/or a thioesterase. - View Dependent Claims (23, 24, 25)
- 2 wherein the amino acid residue corresponding to I231 of SEQ ID BNO;
Specification