Droplet actuators and techniques for droplet-based enzymatic assays
First Claim
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1. A method of conducting an assay, the method comprising:
- (a) incubating a droplet in oil, the droplet comprising an umbelliferone substrate, a sample potentially comprising an enzyme which cleaves the umbelliferone substrate, and a zwitterionic surfactant; and
(b) detecting a signal emitted from the droplet.
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Abstract
Droplet actuators and techniques for droplet-based assays are provided. A method of conducting an assay comprises: incubating a droplet in oil, the droplet comprising an umbelliferone substrate, a sample potentially comprising an enzyme which cleaves the umbelliferone substrate, and a zwitterionic surfactant; and detecting a signal emitted from the droplet.
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Citations
46 Claims
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1. A method of conducting an assay, the method comprising:
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(a) incubating a droplet in oil, the droplet comprising an umbelliferone substrate, a sample potentially comprising an enzyme which cleaves the umbelliferone substrate, and a zwitterionic surfactant; and (b) detecting a signal emitted from the droplet. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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9. A method of conducting a droplet-based enzyme assay, the method comprising:
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(a) providing an immiscible fluid comprising; (i) a sample droplet comprising an enzyme of interest; and (ii) one or more reagent droplets comprising; (1) a substrate which is potentially modified in the presence of the enzyme yielding one or more signal-producing products; (2) a zwitterionic surfactant; and (3) optionally, other reagents sufficient to produce activity of the target enzyme; (b) combining the sample droplet and the one or more reagent droplets in the immiscible fluid to yield a reaction droplet effecting an enzyme reaction in the immiscible fluid; and (c) measuring any signal produced by the one or more signal producing products. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
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Specification