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Global proteomic screening of random bead arrays using mass spectrometry imaging

  • US 9,513,285 B2
  • Filed: 12/17/2013
  • Issued: 12/06/2016
  • Est. Priority Date: 06/30/2010
  • Status: Active Grant
First Claim
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1. A method of detecting the interaction of prey molecules with bait molecules on an array of beads, comprising:

  • a. providing i) a mixture comprising first and second beads, said first beads comprising first bait molecules, said second beads comprising second bait molecules, wherein said first and second bait molecules are different; and

    ii) prey molecules, wherein said prey molecules comprise a detectable label;

    b. contacting said first and second beads with a solution comprising a population of said prey molecules under conditions wherein said prey molecules can bind to a bait molecule;

    c. making a random array with said beads by depositing a mixture of said first and second beads onto a solid surface;

    d. imaging said beads in said array for the presence of said detectable label to detect interaction of prey molecules with bait molecules; and

    e. subjecting said array directly to mass spectrometry measurements under conditions such that one or more individual beads detected by the imaging in step d) are also detected in the same location within the array by said mass spectrometry measurements, wherein said mass spectrometry measurements detect said bait molecules or fragments thereof from said individual beads, individually resolving said beads and allowing identification of said beads in said imaged array.

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