Automated reagent dispensing system and method of operation

• US 9,518,899 B2
• Filed: 02/07/2006
• Issued: 12/13/2016
• Est. Priority Date: 08/11/2003
• Status: Active Grant

• Current Assignee: Sakura Finetek USA Incorporated (Sakura Finetechnical Co., Ltd.)
• Original Assignee: Sakura Finetek USA Incorporated (Sakura Finetechnical Co., Ltd.)
• Inventors: Lefebvre, Gilles, Jennings, Patrick P.
• Primary Examiner(s): Gordon, Brian R
• Application Number:
  US11/349,663
• Publication Number:
  US 20060173575A1
• Time in Patent Office: 3,962 Days
• Field of Search: 422/100, 422/68.1, 422500-501, 422/509, 422/517, 422/521, 422/536, 436/43, 436/46, 436/174, 436/180, 700/236, 700/242, 700244-245, 700265-266, 700/285
• US Class Current: 1/1
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/00881 network configurations
• CPC Class Codes: G01N 2035/0093 random access not determined by physical position
• CPC Class Codes: G01N 35/00584 Control arrangements for automatic analysers
• CPC Class Codes: G01N 35/00871 Communications between instruments or with remote terminals
• CPC Class Codes: G01N 35/0092 Scheduling
• CPC Class Codes: G01N 35/1002 Reagent dispensers

Abstract

A system and method that enables automated reagent dispensing for tissue stainers. The stainers receive staining protocols from a central controller. The central controller may control a plurality of stainers simultaneously. The stainers obtain information provided on slide identifiers which is communicated to the central controller. The central controller determines a particular staining protocol to apply to a particular slide. The staining protocol is downloaded to the stainer which enables the stainer to operate without additional communication with the central controller. A user may manually initiate a staining protocol or the central controller may operate the stainers on a scheduled basis.

Claims

1. A method of dispensing reagent, comprising:

determining an inventory of an automated reagent dispensing system comprising a plurality of stainers, the determining an inventory comprising retrieving information from an identifier on at least one slide retaining tray for retaining a slide in the automated reagent dispensing system, the information identifying a reagent contained in the tray;

downloading, by the automated reagent dispensing system, a processing program from a central controller, the processing program comprising steps for performing an automated staining procedure;

determining, by the automated reagent dispensing system, a reagent staining protocol for reagents based on the identified reagent;

transmitting the determined reagent staining protocol to a manifold controller of the automated reagent dispensing system;

operating, by the manifold controller, the automated reagent dispensing system to perform the determined reagent staining protocol based on the processing program and independently of the central controller and independent of additional communication with the central controller during performance of the reagent staining protocol, wherein the reagent staining protocol comprises a staining process to dispense one or more reagents to a sample in the at least one slide retaining tray; and

dispensing at least one of the reagents from a reagent container into the at least one slide retaining tray according to the reagent staining protocol.

2. The method of claim 1 further wherein determining an inventory further comprises scanning an identifier included on at least one of a reagent container and a waste container that are used in the automated reagent dispensing system.

3. The method of claim 1 further comprising generating a status report corresponding to a status of the automated reagent dispensing system, wherein the automated reagent dispensing system generates the status report.

4. The method of claim 1, further comprising:

determining a reagent container fluid level; and

outputting a reagent container condition signal, wherein the reagent container condition signal indicates an amount of reagent in the reagent container.

5. The method of claim 1 further comprising:

receiving an interrupt signal;

determining whether a hazardous condition exists; and

stopping processing of the processing program upon detection of the hazardous condition.

6. The method of claim 1 further comprising:

activating at least one evacuation port of the slide retaining tray.

7. The method of claim 1 further comprising creating an audit log, wherein creating is performed by the automated reagent dispensing system, wherein the audit log stores information related to the slide retaining tray.

8. The method of claim 1, wherein the automated reagent dispensing system comprises tools for a user to create the processing program, the method further comprising providing the tools for a user, wherein the user creates the processing program with the tools.

9. The method of claim 1, wherein the automated reagent dispensing system comprises tools for a user to modify the processing program, the method further comprising providing the tools for a user, wherein the user modifies the processing program with the tools.

10. The method of claim 5, wherein the hazardous condition includes at least that a reagent having a poisonous gas associated therewith has been dispensed.

11. The method of claim 6, wherein waste evacuated through the activated at least one evacuation port is divided into hazardous and non-hazardous waste with each going into a respective waste container.

12. A method of dispensing reagent, comprising:

determining an inventory of an automated reagent dispensing system comprising a plurality of stainers, the determining an inventory comprising retrieving information from an identifier on at least one slide retaining tray for retaining a slide in the automated reagent dispensing system by the automated reagent dispensing system, the information identifying a reagent contained in the tray;

downloading, by the automated reagent dispensing system, a processing program from a central controller, the processing program comprising steps for performing an automated staining procedure;

determining, by the automated reagent dispensing system, a reagent staining protocol for reagents based on the identified reagent; and

transmitting the reagent staining protocol to a manifold controller of the automated reagent dispensing system;

operating, by the manifold controller, the automated reagent dispensing system based on the processing program to perform the reagent staining protocol and independently of the central controller and independent of additional communication with the central controller during performance of the reagent staining protocol, wherein the reagent staining protocol comprises a staining process to dispense one or more reagents to a sample in the at least one slide retaining tray, the operating comprising;

dispensing at least one of the reagents from a reagent container into the at least one slide retaining tray according to the reagent staining protocol.

13. The method of claim 12 further wherein determining an inventory further comprises scanning an identifier included on at least one of a reagent container and a waste container that are used in the automated reagent dispensing system.

14. The method of claim 12 further comprising generating a status report corresponding to a status of the automated reagent dispensing system, wherein the automated reagent dispensing system generates the status report.

15. The method of claim 12, further comprising:

determining a reagent container fluid level; and

outputting a reagent container condition signal, wherein the reagent container condition signal indicates an amount of reagent in the reagent container.

16. The method of claim 12 further comprising:

receiving an interrupt signal;

determining whether a hazardous condition exists; and

stopping processing of the processing program upon detection of the hazardous condition.

17. The method of claim 12 further comprising:

activating at least one evacuation port of the slide retaining tray.

18. The method of claim 12 further comprising creating an audit log, wherein creating is performed by the automated reagent dispensing system, wherein the audit log stores information related to the slide retaining tray.

19. The method of claim 12, wherein the automated reagent dispensing system comprises tools for a user to create the processing program, the method further comprising providing the tools for a user, wherein the user creates the processing program with the tools.

20. The method of claim 12, wherein the automated reagent dispensing system comprises tools for a user to modify the processing program, the method further comprising providing the tools for a user, wherein the user modifies the processing program with the tools.