System and method for rapid analysis of polycyclic aromatic hydrocarbons
First Claim
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1. A method for detecting one or more polycyclic aromatic hydrocarbons (PAHs) in a sample by means of a CO2-based chromatography analysis comprising:
- providing a sample comprising one or more PAHs for analysis;
wherein the sample is prepared with, extracted or dissolved in a diluent comprising at least 60% organic solvent, with the proviso that the sample is analyzed without a solvent exchange step;
applying the sample to a chromatography column with a solid stationary phase comprising inorganic or hybrid particles having a mean particle size of about 0.5 to about 3.5 microns, wherein said particles have a non-polar or polar/non-polar surface functionality, and wherein the particles retain said one or more PAHs;
eluting the one or more PAHs from the chromatography column by a mobile phase comprising a mixture of liquid CO2 and a modifier to form one or more eluted PAHs;
wherein the mobile phase has a pre-column dwell volume of about 75 μ
L to about 500 μ
L; and
detecting said one or more eluted PAHs.
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Abstract
The subject technology is directed to a CO2-based system and method for rapid determination of the levels and/or the presence or absence of polycyclic aromatic hydrocarbons (PAHs).
29 Citations
29 Claims
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1. A method for detecting one or more polycyclic aromatic hydrocarbons (PAHs) in a sample by means of a CO2-based chromatography analysis comprising:
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providing a sample comprising one or more PAHs for analysis;
wherein the sample is prepared with, extracted or dissolved in a diluent comprising at least 60% organic solvent, with the proviso that the sample is analyzed without a solvent exchange step;applying the sample to a chromatography column with a solid stationary phase comprising inorganic or hybrid particles having a mean particle size of about 0.5 to about 3.5 microns, wherein said particles have a non-polar or polar/non-polar surface functionality, and wherein the particles retain said one or more PAHs; eluting the one or more PAHs from the chromatography column by a mobile phase comprising a mixture of liquid CO2 and a modifier to form one or more eluted PAHs;
wherein the mobile phase has a pre-column dwell volume of about 75 μ
L to about 500 μ
L; anddetecting said one or more eluted PAHs. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A chromatography method for detecting one or more polycyclic aromatic hydrocarbons (PAHs) in a sample comprising the steps of:
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providing a sample comprising one or more PAHs for analysis;
wherein the sample is prepared with, extracted or dissolved in a diluent comprising at least 60% organic solvent, with the proviso that the sample is analyzed without a solvent exchange step;applying the sample to a chromatography column with a solid stationary phase comprising inorganic or hybrid particles having a mean particle size of about 0.5 to about 3.5 microns, wherein said particles have a polar surface functionality, and wherein the particles retain said one or more PAHs; eluting the one or more PAHs from the chromatography column by a mobile phase comprising a liquid CO2 to form one or more eluted PAHs, wherein the mobile phase has a pre-column dwell volume of about 75 μ
L to about 500 μ
L; anddetecting said one or more eluted PAHs by a mass spectrometer.
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28. A method for detecting one or more PAHs comprising:
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(1) providing a sample comprising one or more PAHs for analysis;
wherein the sample is prepared with, extracted or dissolved in a diluent comprising at least 60% organic solvent, with the proviso that the sample is not subject to a solvent exchange step;(2) applying the sample to a chromatography system comprising; (a) a column with a solid stationary phase comprising an inorganic or hybrid particle having a mean particle size of about 0.5 to about 3.5 microns, wherein said particle has a non-polar or polar/non-polar surface functionality, wherein said column has a length of about 50 to 150 mm and an internal diameter about 2 to 4 mm, and wherein the solid stationary phase retains said one or more PAHs; (b) a pre-column mobile phase dwell volume of about 75 μ
L to about 500 μ
L;
wherein said pre-column dwell volume comprises a space within a fluidic connection between a junction at which the CO2 and the modifier are mixed to the head of the column; and(c) a post-column mobile phase dwell volume of about 10 μ
L to about 450 μ
L;
wherein said post-column dwell volume comprises a space within a fluidic connection between the end of the column and a detector;(3) eluting the one or more PAHs from the chromatography column by a mobile phase comprising a mixture of CO2 and a modifier to form one or more eluted PAHs, wherein the mobile phase has a flow rate of about 1 to 4 mL/min; and (4) detecting said one or more eluted PAHs.
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29. A kit for performing analysis or detecting one or more PAHs in a sample comprising:
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a sample preparation device for preparing the sample comprising one or more PAHs for analysis;
wherein the sample is prepared with, extracted or dissolved in a diluent comprising at least about 60% organic solvent, with the proviso that the sample is analyzed without a solvent exchange step;a chromatography column with a solid stationary phase comprising inorganic or hybrid particles having a mean particle size of 0.5 to 3.5 microns;
wherein said particles have a non-polar or polar/non-polar surface functionality and retain said one or more PAHs; andone or more standards for calibrating, and facilitating the analysis and detection of the one or more PAHs.
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Specification