Medical device with coating for capturing genetically-altered cells and methods for using same
First Claim
1. A method of delivering a stent into a patient, comprising the steps of:
- (i) administering genetically-altered endothelial progenitor cells into the patient, said genetically-altered endothelial progenitor cells comprising exogenous nucleic acid encoding a cell membrane marker molecule not normally found in said genetically-altered endothelial progenitor cells, and at least one therapeutic gene product;
said genetically-altered endothelial progenitor cells being capable of expressing said cell membrane marker molecule and said at least one therapeutic gene product, wherein said cell membrane marker molecule is selected from the group consisting of an osteogenic protein, and a prostatic cell membrane protein; and
(ii) separately administering into said patient a cell-free stent comprising a coating on the lumen surface of the stent;
said coating comprising a matrix bearing a covalently attached ligand, wherein said ligand specifically recognizes and binds said cell membrane marker molecule expressed by said genetically-altered endothelial progenitor cells;
wherein the therapeutic gene product is selected from the group consisting of vascular endothelial growth factor, angiogenin, anti-angiogenic factor, and fibroblast growth factor,and wherein said genetically altered endothelial progenitor cells bind to said ligand on said stent in vivo.
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Accused Products
Abstract
Therapeutic and drug delivery systems are provided in the form of medical devices with coatings for capturing and immobilizing target cells such as circulating progenitor or genetically-altered mammalian cells in vivo. The genetically-altered cells are transfected with genetic material for expressing a marker gene and a therapeutic gene in a constitutively or controlled manner. The marker gene is a cell membrane antigen not found in circulating cells in the blood stream and therapeutic gene encodes a peptide for the treatment of disease, such as, vascular disease and cancer. The coating on the medical device may be a biocompatible matrix comprising at least one type of ligand, such as antibodies, antibody fragments, other peptides and small molecules, which recognize and bind the target cells. The therapeutic and/or drug delivery systems may be provided with a signal source such as activator molecules for stimulating the modified cells to express and secrete the desired marker and therapeutic gene products.
1045 Citations
16 Claims
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1. A method of delivering a stent into a patient, comprising the steps of:
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(i) administering genetically-altered endothelial progenitor cells into the patient, said genetically-altered endothelial progenitor cells comprising exogenous nucleic acid encoding a cell membrane marker molecule not normally found in said genetically-altered endothelial progenitor cells, and at least one therapeutic gene product;
said genetically-altered endothelial progenitor cells being capable of expressing said cell membrane marker molecule and said at least one therapeutic gene product, wherein said cell membrane marker molecule is selected from the group consisting of an osteogenic protein, and a prostatic cell membrane protein; and(ii) separately administering into said patient a cell-free stent comprising a coating on the lumen surface of the stent;
said coating comprising a matrix bearing a covalently attached ligand, wherein said ligand specifically recognizes and binds said cell membrane marker molecule expressed by said genetically-altered endothelial progenitor cells;wherein the therapeutic gene product is selected from the group consisting of vascular endothelial growth factor, angiogenin, anti-angiogenic factor, and fibroblast growth factor, and wherein said genetically altered endothelial progenitor cells bind to said ligand on said stent in vivo. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification