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Methods and compositions for efficient base calling in sequencing reactions

  • US 9,523,125 B2
  • Filed: 12/02/2013
  • Issued: 12/20/2016
  • Est. Priority Date: 01/28/2008
  • Status: Active Grant
First Claim
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1. A composition for determining the identity of a base in a target nucleic acid by sequencing-by-synthesis, the composition comprising a mixture of deoxyribonucleotide triphosphates (dNTPs) comprising:

  • (i) first dNTPs comprising a first label;

    (ii) second dNTPs comprising a second label;

    (iii) third dNTPs comprising both the first label and the second label; and

    (iv) fourth dNTPs comprising neither the first label nor the second label;

    wherein each of the first, second, third, and fourth dNTPs is selected from the group consisting of dATP, dTTP, dCTP, and dGTP, and are different from each other; and

    wherein the first label and the second label are different labels and are distinguishable.

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